典型污染物包覆层中氯仿的沿程生物转化机制

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Abstract

It is important to deeply understanding the degradation mechanism of chloroform (CF) along the depth of a soil layer. In this study, the simulated landfill cover system (SLCS) was set up and biotransformation mechanism of CF was firstly investigated by the method of diversity sequencing. The results showed that the landfill cover was divided into three zones along the depth, aerobic zone (0~20cm), anoxic zone (20~40cm) and anaerobic zone (>40cm), based on the oxygen content. The analysis of diversity sequencing revealed that methanotrophs were dominant bacteria in aerobic zone, including type I methanotrophs Methylobacter and type Ⅱ methanotrophs Methylosinus. While Anaeromyxobacter was the dominant bacteria for CF biodegradation by reductive dechlorination in anoxic and anaerobic zone. In addition, the relative abundance of methanotrophs in the anoxic zone was about 13%. These results suggested that CF was firstly degraded effectively in aerobic, anoxic and anoxic zone. Previous studies reported that CF can be degraded into dichloromethane reductive dechlorination in the anoxic and anaerobic zone, and part of dichloromethane would be transformed into acetate, H₂ and CO₂ due to the activity of Dehalobacter, which agreed with this study. The metabolic product dichloromethane was then completely degraded by through co-metabolism by methanotrophs in aerobic and anoxic zone. Moreover, the relationship between biodegradation capacity and gas flux was also studied. With the change of inlet flux, there was a negative correlation between methane removal efficiency and flux (R²=0.80), while the positive correlation was found between methane biodegradation rate and flux (R²=0.86). Similarly, CF biodegradation efficiency decreased with increase of inlet gas flux (R²=0.86), while biodegradation rate increased with increase of inlet gas flux (R²=0.89). Therefore, aerobic co-metabolism contributed more in in removal of CF than reductive dichlorination. These results provided theoretical basis for in situ bioremediation of chlorinated aliphatic hydrocarbons pollutants.

Key words： landfill cover chloroform reductive dechlorination aerobic cometabolism

Received: 10 May 2018

PACS:

X701

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	Articles by authors
	LIU Shuai
	XING Zhi-lin
	LI Chen
	ZHANG Hao
	HU Wen-qing
	ZHANG Li-jie
	ZHANG Yun-ru
	ZHAO Tian-tao

Cite this article:

LIU Shuai,XING Zhi-lin,LI Chen等. The biotransformation mechanism of chloroform in landfill cover[J]. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(12): 4581-4590.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2018/V38/I12/4581

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