Integrating the red line of ecological conservation, the bottom line of environmental quality security, the upper line of natural resource utilization, and the blacklist of activities for environmental scrutiny (requirement of three lines and one list, TLOL) is a potentially effective way to the implementation of SEA. However, how to incorporate the requirement of TLOL into SEA is still lack of experience. This study explored the evaluation content and the functional localization of the TLOL in the main assessment of urban development SEA, an evaluation technical system of urban development SEA has constructed based on TLOL from both aspects of key tasks and technical process, the green development SEA of Ordos has been taken as an example. The case study showed that for cities such as Ordos with a large space scale and a high heterogeneity of natural environment and industrial development, this evaluation system could effectively prevent and solve the environmental pollution, ecological degradation and inefficient resource allocation which caused by the urban sprawl and the imbalance industrial structure during the implementation of the strategy. Moreover, it could improve the management and control of differentiated environment in urban classification and zoning.