垃圾渗滤液与再生水生物毒性的体外检测方法应用

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Abstract Main in vitro models and in vitro assays for biotoxicity detection of landfill leachate and reclaimed water, and the application of these models and assays in the evaluation of biotoxicity were summarized. The commonly used in vitro models included human-derived cells, other mammalian cells, and microbial cells. In comparison, results obtained from human-derived cells were more persuasive when extrapolated to humans, which led to more widely application of human-derived cell models. One the other hand, in vitro assays included three aspects: detections of cell toxicity, genotoxicity, and endocrine disturbing effects. Among them, studies on endocrine disturbing effects focused more on estrogenic effects nowadays. Moreover, this paper proposed that the development of three-dimensional in vitro model, combination of in vitro assays with chemical analysis, more comprehensive analyses of endocrine disrupting effects, and establishment of group test system of in vitro assays could be the potential future directions for the development of biotoxicity assays of landfill leachate and reclaimed water.

Key words： landfill leachate reclaimed water biotoxicity in vitro models in vitro assays

Received: 09 February 2020

PACS:

X171.5

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	Articles by authors
	XU Qian-ru
	ZHAO Fei
	XIA Na-na
	LI Xin-hui
	GAO Xu-chao
	YANG Yong-chuan
	BI Xue-jun

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XU Qian-ru,ZHAO Fei,XIA Na-na等. Application of in vitro assays in the biotoxicity detection of landfill leachate and reclaimed water[J]. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(9): 3887-3893.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2020/V40/I9/3887

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