不同饮食习惯下镉摄入对肠道菌群结构的影响

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (942 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract To investigate the effects of cadmium (Cd) exposure on gut microbiota structures under different dietary habits. The C57BL/6 mice were treated with Normal Diet (ND), Normal diet+10 mg/L CdCl₂(ND+Cd), High-Fat Diet (HFD), or High-Fat Diet+10 mg/L CdCl₂(HFD+Cd), respectively for 20 weeks. Subsequently, the changes of composition and structure of the gut microbiota were analyzed via 16S rRNA high-throughput sequencing technology. The results indicate that HFD and Cd caused intestinal disorders. Compared with the ND group, HFD intake decreased Bacteroidetes abundance by 2.28 times, and the Bifidobacterium number increased by 14.34 times. Cd intake increased the abundance of Verrucomicrobia, dominated by Akkermansia to about 4.67% of gut microbiota. Compared with the HFD group, the abundance of Actinobacteria in the HFD+Cd group decreased by 4.57 times, RuminococcaceaeUCG-014, Alistipes, Lachnospiraceae_NK4A136_gup, Blautia, and Desulfovibrio being the dominant flora. Studies have shown that HFD or/and Cd can disrupt the balance of gut microbiota, and the intake of Cd under different dietary habits shows a different flora structure, which is reflected in each classification level.

Key words： 16S rRNA high fat diet cadmium gut microbiota<

Received: 07 January 2021

PACS:

X503.1

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	QIN Yi-shu
	BAO Xin-chen
	WANG Jie
	YU Miao
	TIAN Wen
	ZHANG Meng-yan
	XIANG Ping

Cite this article:

QIN Yi-shu,BAO Xin-chen,WANG Jie等. Effect of cadmium intake on the structure of gut microbiota under different dietary habits[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(8): 3896-3905.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I8/3896

[1]	Zwolak A, Magdalena S, Szpyrka E, et al. Sources of Soil Pollution by Heavy Metals and Their Accumulation in Vegetables:a Review[J]. Water Air and Soil Pollution, 2019,230(7):164-172.
[2]	Qin G W, Niu Z D, Yu J D, et al. Soil heavy metal pollution and food safety in China:effects, sources and removing technology[J]. Chemosphere, 2020,267:129205.
[3]	李梦莹,王成尘,毕珏,等.食品中重金属的人体健康风险评估方法研究进展[J]. 福建农林大学学报(自然科学版), 2021,50(1):1-9. Li M Y, Wang C C, Bi J, et al. Human health risk assessment of heavy metals in food:a review[J]. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2021,50(1):1-9.
[4]	全国土壤污染状况调查公报[J]. 中国环保产业, 2014,(5):10-11. The report on the national general survey of soil contamination[J]. China Environmental Protection Industry, 2014, (5):10-11.
[5]	Cristina, Nerín, Margarita, et al. Food contamination during food process[J]. Trends in Food Science & Technology, 2016,48:63-68.
[6]	Sathyamoorthy K, Sivaruban T, Barathy S. Assessment of heavy metal pollution and contaminants in the cattle meat[J]. Jr. of Industrial Pollution Control, 2016,32(1):335-355.
[7]	Rodriguez-Estival J, Morales-Machuca C, Pareja-Carrera J, et al. Food safety risk assessment of metal pollution in crayfish from two historical mining areas:Accounting for bioavailability and cooking extractability[J]. Ecotoxicology and Environmental Safety, 2019, 185:109682.
[8]	Keshavarzi B, Hassanaghaei M, Moore F, et al. Heavy metal contamination and health risk assessment in three commercial fish species in the Persian Gulf[J]. Marine Pollution Bulletin, 2018, 129(1):245-252.
[9]	Cai L M, Wang Q S, Luo J, et al. Heavy metal contamination and health risk assessment for children near a large Cu-smelter in central China[J]. The Science of the total environment, 2019,650:725-733.
[10]	Sharma S, Nagpal A K, Kaur I. Heavy metal contamination in soil, food crops and associated health risks for residents of Ropar wetland, Punjab, India and its environs[J]. Food Chemistry, 2018,255:15-22.
[11]	Wang K, Ma J Y, Li M Y, et al. Mechanisms of Cd and Cu induced toxicity in human gastric epithelial cells:Oxidative stress, cell cycle arrest and apoptosis[J]. Science of The Total Environment, 2020, 143951.
[12]	Round J L, Mazmanian S K. Erratum:The gut microbiota shapes intestinal immune responses during health and disease[J]. Nature Reviews Immunology, 2009,9(8):600.
[13]	Liu Y H, Li Y H, Xia Y H, et al. The dysbiosis of gut microbiota caused by low-dose cadmium aggravate the injury of mice liver through increasing intestinal permeability[J]. Microorganisms, 2020, 8(2):211-224.
[14]	Ba Q, Li M, Chen P. Sex-dependent effects of cadmium exposure in early life on gut microbiota and fat accumulation in mice[J]. Environmental health perspectives, 2017,125(3):437-446.
[15]	Zhai Q X, Li T Q, Yu L L, et al. Effects of subchronic oral toxic metal exposure on the intestinal microbiota of mice[J]. Science Bulletin, 2017,62(12):831-840.
[16]	Kyung-Ah K, Wan G, In-Ah L, et al. High fat diet-induced mut microbiota exacerbates Inflammation and obesity in mice via the TLR4signaling pathway[J]. Plos One, 2012,7(10):e47713.
[17]	Cani P D, Neyrinck A M, Fava F, et al. Selective increases of bifidobacteria in gut microflora improve high-fat-diet-induced diabetes in mice through a mechanism associated with endotoxaemia[J]. Diabetologia, 2007,50(11):2374-2383.
[18]	Huang L N, Wang Z H, Wang H J, et al. Nutrition transition and related health challenges over decades in China[J]. European Journal of Clinical Nutrition, 2020,75(10B):1-6.
[19]	Park S S, Skaar D A, Jirtle R L, et al. Epigenetics, obesity and early-life cadmium or lead exposure[J]. Epigenomics, 2017,9(1):57-75.
[20]	Jonathan B. Availability of high-fat foods might drive the obesity epidemic[J]. Nature Reviews Endocrinology, 2018,14(10):574-575.
[21]	Li X J, Brejnrod A D, Ernst M, et al. Heavy metal exposure causes changes in the metabolic health-associated gut microbiome and metabolites[J]. Environment International, 2019,126:454-467.
[22]	He X W, Qi Z D, Hou H, et al. Structural and functional alterations of gut microbiome in mice induced by chronic cadmium exposure[J]. Chemosphere, 2020,246:125747.
[23]	Nguyen N P, Warnow T, Pop M, et al. A perspective on 16S rRNA operational taxonomic unit clustering using sequence similarity[J]. NPJ Biofilms and Microbiomes, 2016,2:16004.
[24]	Lu Y Y, Chen J, Zheng J Y, et al. Mucosal adherent bacterial dysbiosis in patients with colorectal adenomas[J]. Scientific Reports, 2016, 6:26337.
[25]	Cani P D, Bibiloni R, Knauf C, et al. Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice[J]. Diabetes, 2008,57(6):1470-1481.
[26]	Serre C B D L, Ellis C L, Lee J, et al. Propensity to high-fat diet-induced obesity in rats is associated with changes in the gut microbiota and gut inflammation[J]. American Journal of Physiology-Gastrointestinal and Liver Physiology, 2010,299(2):G440-G448.
[27]	Zhang C, Zhang M, Pang X, et al. Structural resilience of the gut microbiota in adult mice under high-fat dietary perturbations[J]. The Isme Journal, 2012,6(10):1848-1857.
[28]	Konkel, Lindsey. The environment within:exploring the role of the gut microbiome in health and disease[J]. Environmental Health Perspectives, 2013,121(9):A276-A281.
[29]	Carding S, Verbeke K, Vipond D T, et al. Dysbiosis of the gut microbiota in disease[J]. Microbial Ecology in Health & Disease, 2015,26(1):26191.
[30]	Degruttola A K, Daren L, Atsushi M, et al. Current understanding of dysbiosis in disease in human and animal models[J]. Inflammatory Bowel Diseases, 16,22(5):1137-1150.
[31]	Ley R E, Backhed F, Turnbaugh P, et al. Obesity alters gut microbial ecology[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005,102(31):11070-11075.
[32]	Vicente R L, Cook R L, Sobel J D. Emerging role of lactobacilli in the control and maintenance of the vaginal bacterial microflora[J]. Reviews of Infectious Diseases, 1990,(5):856-872.
[33]	Hildebrandt M A, Hoffmann C, Sherrill-Mix S A, et al. High-fat diet determines the composition of the murine gut microbiome independently of obesity[J]. Gastroenterology, 2009,137(5):1716-1724.
[34]	Daniel H, Gholami A M, Berry D, et al. High-fat diet alters gut microbiota physiology in mice[J]. The ISME Journal, 2014,8(2):295-308.
[35]	Rohr M W, Narasimhulu C A, Rudeski-Rohr T A, et al. Negative effects of a high-fat diet on intestinal permeability:a review[J]. Advances in Nutrition, 2019,11(1):1-15.
[36]	Arumugam M, Raes J, Pelletier E, et al. Enterotypes of the human gut microbiome[J]. Nature, 2011,473(7346):174-180.
[37]	Huttenhower C, Gevers D, Knight R, et al. Structure, function and diversity of the healthy human microbiome[J]. Nature, 2012, 486(7421):207-214.
[38]	Nguyen T L A, Vieira-Silva S, Liston A, et al. How informative is the mouse for human gut microbiota research?[J]. Disease Models and Mechanisms, 2015,8(1):1-16.
[39]	John G K, Mullin G E. The gut microbiome and obesity[J]. Current Oncology Reports, 2016,18(7):45-51.
[40]	Zarrinpar A, Chaix A, Yooseph S, et al. Diet and Feeding Pattern Affect the Diurnal Dynamics of the Gut Microbiome[J]. Cell Metabolism, 2014,20(6):1006-1017.
[41]	Ley R E, Turnbaugh P J, Klein S, et al. Human gut microbes associated with obesity[J]. Nature, 2006,444(7122):1022-1023.
[42]	Turnbaugh P J, Hamady M, Yatsunenko T, et al. A core gut microbiome in obese and lean twins[J]. Nature, 2009,457(7228):480-484.
[43]	Turnbaugh P J, Ley R E, Mahowald M A, et al. An obesity-associated gut microbiome with increased capacity for energy harvest[J]. Nature, 2006,444(7122):1027-1031.
[44]	Hekmatdoost A, Feizabadi M M, Djazayery A, et al. The effect of dietary oils on cecal microflora in experimental colitis in mice[J]. Indian Journal of Gastroenterology Official Journal of the Indian Society of Gastroenterology, 2008,27(5):156-189.
[45]	赵立平,张晨虹,杨鑫.微生物组研究热潮中的冷思考[J]. 生命科学, 2017,29(7):619-623. Zhao L P, Zhang C H, Yang X. The cold thinking in the upsurge of microbiome research[J]. Chinese Bulletin of Life Sciences, 2017, 29(7):619-623.
[46]	徐仁应,万燕萍,方启宇,等.高脂饮食对大鼠肠道益生菌和肝脂肪含量的影响[J]. 营养学报, 2011,33(4):367-369,375. Xu R Y, Wan Y P, Fang Q Y, et al. Effects of high fat diet on gut probiotics and hepatic fat in rat[J]. ACTA Nutrimenta SINICA, 2011,33(4):367-369,375.
[47]	白爱平,欧阳钦,胡仁伟.双歧杆菌抑制小鼠实验性结肠炎肠道炎症反应的研究[J]. 中华消化杂志, 2005,25(6):344-347. Bai A P, Ou Y Q, Hu R W. The research on bifidobacterium inhibits inflammatory response in mutine experimental colitis[J]. Chinese Journal of Digestion, 2005,25(6):344-347.
[48]	Diaz-Bone R A, Wiele T V D. Biotransformation of metal(loid)s by intestinal microorganisms[J]. Pure & Applied Chemistry, 2010, 82(2):409-427.
[49]	Duan H, Yu L L, Tian F W, et al. Gut microbiota:A target for heavy metal toxicity and a probiotic protective strategy[J]. Science of The Total Environment, 2020,742:140429.
[50]	Assefa S, Köhler G. Intestinal microbiome and metal toxicity[J]. Current Opinion in Toxicology, 2020,19:21-27.
[51]	Zhang S B, Jin Y X, Zeng Z Y, et al. Subchronic exposure of mice to cadmium perturbs their hepatic energy metabolism and gut microbiome[J]. Chemical Research in Toxicology, 2015,28(10):2000-2009.
[52]	Liu Y H, Li Y H, Liu K Y, et al. Exposing to cadmium stress cause profound toxic effect on microbiota of the mice intestinal tract[J]. Plos One, 2014,9(2):e85323.
[53]	Fazeli M, Hassanzadeh P, Alaei S. Cadmium chloride exhibits a profound toxic effect on bacterial microflora of the mice gastrointestinal tract[J]. Human & Experimental Toxicology, 2011, 30(2):152-159.
[54]	Breton J, Massart S, Vandamme P, et al. Ecotoxicology inside the gut:impact of heavy metals on the mouse microbiome[J]. BMC Pharmacology & Toxicology, 2013,14(1):62-72.
[55]	董新燕,刘雪,刘曼妮,等.嗜粘蛋白阿克曼菌在糖尿病和肥胖中的作用[J]. 微生物学报, 2020,60(5):19-26. Dong X Y, Liu X, Liu M N, et al. Role of Akkermansia muciniphila in diabetes and obesity[J]. Acta microbiologica Sinica, 2020,60(5):19-26.
[56]	Depommier C, Everard A, Druart C, et al. Supplementation with Akkermansia muciniphila in overweight and obese human volunteers:a proof-of-concept exploratory study[J]. Nature Medicine, 2019,25(7):1096.
[57]	Zhao S Q, Liu W, Wang J Q, et al. Akkermansia muciniphila improves metabolic profiles by reducing inflammation in chow diet-fed mice[J]. Journal of Molecular Endocrinology, 2016,58(1):JME-16-0054.
[58]	Everard A, Belzer C, Geurts L, et al. Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity[J]. Proceedings of the National Academy of Sciences of the United States of America, 2013,110(22):9066-9071.
[59]	Qin J J, Li Y R,Cai Z M, et al. A metagenome-wide association study of gut microbiota in type 2diabetes[J]. Nature, 2012,490(7418):55-60.
[60]	Chassaing B, Koren O, Goodrich J K, et al. Dietary emulsifiers impact the mouse gut microbiota promoting colitis and metabolic syndrome[J]. Nature, 2015,000:1-18.
[61]	Liu T, Liang X, Lei C, et al. High-fat diet affects heavy metal accumulation and toxicity to mice liver and kidney probably via gut microbiota[J]. Frontiers in Microbiology, 2020,11:1604.
[62]	Saulnier D M, Riehle K, Mistretta T A. Gastrointestinal microbiome signatures of pediatric patients with irritable bowel syndrome[J]. Gastroenterology, 2011,141(5):1782-1791.
[63]	Yang J, Zheng P, Li Y F, et al. Landscapes of bacterial and metabolic signatures and their interaction in major depressive disorders[J]. Science Advances, 2020,6(49):eaba8555.