|
|
|
| Lead-induced alterations in hepatocyte biomolecules based on Raman spectroscopy |
| XING Yu1,2, PANG Wei-yi2, XU Li1 |
1. Institute of Quality Standard and Testing Technology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
2. School of Public Health, Guilin Medical University, Guilin 541199, China |
|
|
|
|
Abstract In this study, we employed Raman spectroscopy to explore the bio-molecular alterations in HepG2cells following exposure to lead with concentrations of 0.05, 0.1, 0.5, 1, 5mg/L, which took different time periods (0.5, 1.5, 3, 6, 12, 24h) respectively. Raman spectra correlated to cellular biological information was collected via Raman microscopy to further investigate the bio-molecular alterations of liver cells community following lead exposure. It showed that although the spectra in each treatment group were similar, certain diversities of the peaks positions and the absorption intensities were observed. Additionally, multivariate analysis was applied to the spectral dataset to determine the biological changes underlying the exposure, and it showed that the spectral data of cellular groups following exposures at different concentrations with the same exposure time showed a discrete trend on LD1, and it was significantly observed in 24-h exposure. The results, as well, indicated that lead exposure can damage the structure of liver cell proteins, lipids, nucleic acids, carotenoids, carbohydrates and affect their normal functions. These cellular responses are dose-related and increases with exposure time. In conclusion, our study suggests that Raman spectroscopy is proper tool with ability to detect the biological molecular changes in liver cells following lead exposure. It can not only provide a new assay in toxicology research, but also provides a theoretical basis for rapid safety evaluation of environmental pollutants.
|
|
Received: 14 October 2021
|
|
|
|
|
|
| [1] |
Hou S N, Hua X Y, Ji X F, et al. Pollution characteristics, sources, and health risk assessment of human exposure to Cu, Zn, Cd and Pb pollution in urban street dust across China between 2009 and 2018 [J]. Environment International, 2019,128:430-437.
|
| [2] |
Dong Z M, Wu S M, Hu J Y,et al. Health risk assessment for children due to lead exposure in some region of China [J]. China Environmental Science, 2011,31(11):1910-1916.
|
| [3] |
Zou T S, Zhang J L, Chen Y, et al. Contents and distribution characteristics of atmospheric lead in urban China [J]. China Environmental Science, 2015,35(1):23-32.
|
| [4] |
Kasperczyk S, Blaszczyk I, Dobrakowski M, et al. Exposure to lead affects male biothiols metabolism [J]. Annals of Agricultural and Environmental Medicine, 2013,20(4):721-725.
|
| [5] |
Labudda M. Lead Hepatotoxicity: selected aspects of pathobiochemistry [J]. Medycyna Pracy, 2013,64(4):565-568.
|
| [6] |
Xu L, Wei Q, Li J Y, et al. Research progress on toxic effects of pollutants based on spectrum technology [J]. Asian Journal of Ecotoxico1ogy, 2018,13(6):50-60.
|
| [7] |
Liu Y J, Kyne M, Wang C, et al. Data mining in Raman imaging in a cellular biological system [J]. Computational and Structural Biotechnology Journal, 2020,18:2920-2930.
|
| [8] |
Trevisan J, Angelov P P, Scott A D, et al. IRootLab: a free and open-source MATLAB toolbox for vibrational biospectroscopy data analysis [J]. Bioinformatics, 2013,29(8):1095-1097.
|
| [9] |
Kumamoto Y, Harada Y, Takamatsu T, et al. Label-free Molecular Imaging and Analysis by Raman Spectroscopy [J]. Acta Histochemica Et Cytochemica, 2018,51(3):101-110.
|
| [10] |
Tian D Y, Lu G D, Zhai Z G, et al. Rapid thyroid dysfunction screening based on serum surface-enhanced Raman scattering and multivariate statistical analysis [J]. Laser Physics Letters, 2018,15(1):5.
|
| [11] |
Li C, Shi L, Peng C, et al. Lead-induced cardiomyocytes apoptosis by inhibiting gap junction intercellular communication via autophagy activation [J]. Chemico-Biological Interactions, 2021,337:8.
|
| [12] |
Hosseinirad H, Shahrestanaki J K, Moghaddam M M, et al. Protective Effect of Vitamin D3Against Pb-Induced Neurotoxicity by Regulating the Nrf2 and NF-kappa B Pathways [J]. Neurotoxicity Research, 2021,39(3):687-696.
|
| [13] |
Metryka E, Kupnicka P, Kapczuk P, et al. Lead (Pb) Accumulation in Human THP-1Monocytes/Macrophages In Vitro and the Influence on Cell Apoptosis [J]. Biological Trace Element Research, 2021,199(3): 955-967.
|
| [14] |
Belatik A, Hotchandani S, Carpentier R, et al. Locating the Binding Sites of Pb(II) Ion with Human and Bovine Serum Albumins [J]. Plos One, 2012,7(5):9.
|
| [15] |
Cole T R, Erickson S G, Morales K A, et al. Cd(II)- and Pb(II)-Induced Self-Assembly of Peripheral Membrane Domains from Protein Kinase C [J]. Biochemistry, 2019,58(6):509-513.
|
| [16] |
Dowd T L, Li L, Gundberg C M. The H-1NMR structure of bovine Pb2+-osteocalcin and implications for lead toxicity [J]. Biochimica Et Biophysica Acta-Proteins and Proteomics, 2008,1784(11):1534-1545.
|
| [17] |
Darwish W S, Ikenaka Y, Nakayama S M M, et al. Constitutive Effects of Lead on Aryl Hydrocarbon Receptor Gene Battery and Protection by beta-carotene and Ascorbic Acid in Human HepG2Cells [J]. Journal of Food Science, 2016,81(1):275-281.
|
| [18] |
Zhang Y F, Deng B L, Li Z T. Inhibition of NADPH oxidase increases defense enzyme activities and improves maize seed germination under Pb stress [J]. Ecotoxicology and Environmental Safety, 2018,158:187- 192.
|
| [19] |
Sanchez-Valle V, Valverde M, Carrizales L, et al. A metal mixture induces transformation upon antioxidant depletion in a hepatic cell line [J]. Annals of Hepatology, 2013,12(2):315-324.
|
| [20] |
Zhang H, Wei K, Zhang M Y, et al. Assessing the mechanism of DNA damage induced by lead through direct and indirect interactions [J]. Journal of Photochemistry and Photobiology B-Biology, 2014,136: 46-53.
|
| [21] |
Shilpa O, Anupama K P, Antony A, et al. Lead (Pb) induced Oxidative Stress as a Mechanism to Cause Neurotoxicity in Drosophila melanogaster [J]. Toxicology, 2021,462:152959.
|
|
|
|
