Effect of drainage rate on the enhancement of the complete autotrophic nitrogen removal over nitrite process in a tidal flow constructed wetland
JIN Hui-zheng1, WANG Zhen2, DING Ya-nan2
1. Henan Technical College of Construction, Zhengzhou 450007, China; 2. Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
Abstract：This study attempted to achieve a high-rate nitrogen removal via the complete autotrophic nitrogen removal over nitrite (CANON) process in a tidal flow constructed wetland (TFCW), thus nitrogen transformation mechanisms and the related microbiological characteristics in the TFCWs treating domestic wastewater were explored under drainage rate (vd) constraints. The results showed that, vd significantly affected quantities and activities of the functional microbes in the TFCWs. Correspondingly, nitrogen transformation rates in the systems fluctuated at the different five levels of vd. As the vd decreased from 1.00 to 0.50L/min, the oxygen-limiting microenvironment gradually formed in the TFCW, which was conductive to the stabilization of nitritation and the enrichment of anammox. Subsequently, enhancement of the CANON process was achieved in the TFCW as a result of appropriate vd. However, as the vd was lower than 0.50L/min, the activity of aerobic ammonia-oxidizing bacteria (AOB) was inhibited and its quantity was also insufficient because of the insufficient of dissolved oxygen (DO) in the bed, leading to an unsatisfactory effect for nitrogen removal of the TFCW. When the vd was 0.50L/min, the CANON process could be enhanced most effectively in the system, and the mean TN and NH4+-N removal rates reached up to (116.79±13.16) and (102.75±4.35)mg/(L·d), respectively. Overall, autotrophic nitrogen removal via CANON process developed in the TFCW as a result of appropriate vd, facilitating establishment of the TFCW with CANON process.
靳慧征, 王振, 丁亚男. 排水速率对潮汐流人工湿地中CANON作用的强化[J]. 中国环境科学, 2018, 38(6): 2182-2192.
JIN Hui-zheng, WANG Zhen, DING Ya-nan. Effect of drainage rate on the enhancement of the complete autotrophic nitrogen removal over nitrite process in a tidal flow constructed wetland. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(6): 2182-2192.
Wu S, Kuschk P, Brix H, et al. Development of constructed wetlands in performance intensifications for wastewater treatment:A nitrogen and organic matter targeted review[J]. Water Research, 2014,57:40-55.
[2]
Vymazal J. Constructed wetlands for wastewater treatment:Five decades of experience[J]. Environmental Science and Technology, 2011,45:61-69.
[3]
Ilyas H, Masih I. The performance of the intensified constructed wetlands for organic matter and nitrogen removal:A review[J]. Journal of Environmental Management, 2017,198:372-383.
[4]
Vymazal J. The use of hybrid constructed wetlands for wastewater treatment with special attention to nitrogen removal:A review of a recent development[J]. Water Research, 2013,47(14):4795-4811.
[5]
Zhang L, Zhang S, Peng Y, et al. Nitrogen removal performance and microbial distribution in pilot-and full-scale integrated fixed-biofilm activated sludge reactors based on nitritation-anammox process[J]. Bioresource Technology, 2015,196:448-453.
[6]
Khin T, Annachhatre A P. Novel microbial nitrogen removal processes[J]. Biotechnology Advances, 2004,22(7):519-532.
[7]
Huang M, Wang Z, Qi R. Enhancement of the complete autotrophic nitrogen removal over nitrite process in a modified single-stage subsurface vertical flow constructed wetland:Effect of saturated zone depth[J]. Bioresource Technology, 2017,233:191-199.
[8]
Wang Z, Huang M, Qi R, et al. Enhancing nitrogen removal via the complete autotrophic nitrogen removal over nitrite process in a modified single-stage tidal flow constructed wetland[J]. Ecological Engineering, 2017,103:170-179.
[9]
Wang Z, Huang M, Qi R, et al. Enhanced nitrogen removal and associated microbial characteristics in a modified single-stage tidal flow constructed wetland with step-feeding[J]. Chemical Engineering Journal, 2017,314:291-300.
[10]
Sun G, Austin D. Completely autotrophic nitrogen-removal over nitrite in lab-scale constructed wetlands:Evidence from a mass balance study[J]. Chemosphere, 2007,68(6):1120-1128.
[11]
Hu Y, Zhao X, Zhao Y. Achieving high-rate autotrophic nitrogen removal via Canon process in a modified single bed tidal flow constructed wetland[J]. Chemical Engineering Journal, 2014, 237:329-335.
[12]
Wen J, Tao W, Wang Z, et al. Enhancing simultaneous nitritation and anammox in recirculating biofilters:Effects of unsaturated zone depth and alkalinity dissolution of packing materials[J]. Journal of Hazardous Materials, 2013,244:671-680.
[13]
Wu S, Zhang D, Austin D, et al. Evaluation of a lab-scale tidal flow constructed wetland performance:Oxygen transfer capacity, organic matter and ammonium removal[J]. Ecological Engineering, 2011,37(11):1789-1795.
Wang Z, Liu C, Liao J, et al. Nitrogen removal and N2O emission in subsurface vertical flow constructed wetland treating swine wastewater:Effect of shunt ratio[J]. Ecological Engineering, 2014,73:446-453.
Ji G, Zhi W, Tan Y. Association of nitrogen micro-cycle functional genes in subsurface wastewater infiltration systems[J]. Ecological Engineering, 2012,44:269-277.
[21]
Zhi W, Ji G. Quantitative response relationships between nitrogen transformation rates and nitrogen functional genes in a tidal flow constructed wetland under C/N ratio constraints[J]. Water Research, 2014,64:32-41.
[22]
Wang Z, Dong J, Liu L, et al. Screening of phosphate-removing substrates for use in constructed wetlands treating swine wastewater[J]. Ecological Engineering, 2013,54:57-65.
Nielsen M, Bollmann A, Sliekers O, et al. Kinetics, diffusional limitation and microscale distribution of chemistry and organisms in a CANON reactor[J]. FEMS Microbiology Ecology, 2005, 51(2):247-256.
[26]
Pynaert K., Smets B F, Wyffels S, et al. Characterization of an autotrophic nitrogen-removing biofilm from a highly loaded lab-scale rotating biological contactor. Applied & Environmental Microbiology, 2003,69(6):3626-3635.
Poly F, Wertz S, Brothier E, et al. First exploration of Nitrobacter diversity in soils by a PCR cloning-sequencing approach targeting functional gene nxrA[J]. FEMS Microbiology Ecology, 2007,63(1):132-140.
[30]
Zhu X, Chen Y. Reduction of N2O and NO generation in anaerobic-aerobic (low dissolved oxygen) biological wastewater treatment process by using sludge alkaline fermentation liquid[J]. Environmental Science & Technology, 2011,45(6):2137-2143.
[31]
Bru D, Sarr A, Philippot L. Relative abundances of proteobacterial membrane-bound and periplasmic nitrate reductases in selected environments[J]. Applied and Environmental Microbiology, 2007,73(18):5971-5974.
[32]
Yan T, Fields M W, Wu L, et al. Molecular diversity and characterization of nitrite reductase gene fragments (nirK and nirS) from nitrate-and uranium-contaminated groundwater[J]. Environmental Microbiology, 2003,5(1):13-24.
[33]
Kandeler E, Deiglmayr K, Tscherko D, et al. Abundance of narG, nirS, nirK, and nosZ genes of denitrifying bacteria during primary successions of a glacier foreland[J]. Applied & Environmental Microbiology, 2006,72(9):5957-5962.
[34]
Braker G, Tiedje J M. Nitric oxide reductase (norB) genes from pure cultures and environmental samples[J]. Applied and Environmental Microbiology, 2003,69(6):3476-3483.
[35]
Stres B, Mahne I, Avguštin G, et al. Nitrous oxide reductase (nosZ) gene fragments differ between native and cultivated Michigan soils[J]. Applied and Environmental Microbiology, 2004,70(1):301-309.
[36]
Wang S, Zhu G, Peng Y, et al. Anammox bacterial abundance, activity, and contribution in riparian sediments of the Pearl River estuary[J]. Environmental Science & Technology, 2012,46(16):8834-8842.
