qPCR reveals the succession of syntrophic propionate-degrading consortia with OLR increase
ZHANG Li-guo1, LI Yan-lin1, BAN Qiao-ying1, LI Jian-zheng2
1. College of Environment and Resource, Shanxi University, Taiyuan 030006, China;
2. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China
To reveal the effects of organic loading rate (OLR) on the performance of upflow anaerobic sludge bed (UASB) reactor and syntrophic propionate-degrading consortia, this study investigated the effects of OLR increase on the treatment efficiency of UASB containing sugar refinery wastewater as substrate. The succession of syntrophic propionate-degrading consortia as OLR increasing was analyzed by quantitative real-time fluorescence polymerase chain reaction (qPCR). The results showed that the COD removal reached above 92.0% in UASB at OLR of 6.0~54.0kgCOD/(m3·d) conditions. qPCR explored that at least three identified species of propionate-oxidizing bacteria (Pelotomaculum schinkii, P. propionicum, and Syntrophobacter sulfatireducens) existed in the UASB system. S. sulfatireducens was dominated in UASB and its quantity was 126~1.2×103 16S rDNA copies per ng DNA, accounting for 47.9%~58.6% of the total detectable propionate-oxidizing bacteria. OLR increase from 6.0 to 54.0kgCOD/(m3·d) resulted in all detected propionate-oxidizing bacteria significantly decreased. In addition, Methanospirillum hungatei and Methanosaeta concilii were the dominant hydrogenotrophic methanogens and acetotrophic methanogens. The number of methanogens was significantly increased with OLR increase and achieved a maximum at OLR of 54.0kgCOD/(m3·d) condition.
张立国, 李彦霖, 班巧英, 李建政. qPCR揭示丙酸降解菌群随OLR提高的演替规律[J]. 中国环境科学, 2018, 38(8): 2997-3002.
ZHANG Li-guo, LI Yan-lin, BAN Qiao-ying, LI Jian-zheng. qPCR reveals the succession of syntrophic propionate-degrading consortia with OLR increase. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(8): 2997-3002.
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