Variations of Antibiotic resistance genes during lincomycin mycelia dreg composting
REN Sheng-tao1, GUO Xia-li1, LU A-qian1, ZHANG Qian-qian1, GUO Xiao-ying1, WANG Yan1, WANG Lian-zhong2, ZHANG Bao-bao2
1. College of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou 450001, China;
2. Henan Xinxiang Hua Xing Pharmaceutical Factory, Xinxiang 453731, China
In order to investigate the variation of antibiotic resistance genes during the antibiotic mycelia dregs composting process, five antibiotic resistance genes (lnuA-01, sul1, ermA, ermB, ermC) and one horizontal gene transfer (intI1) were detected from the compost by quantitative PCR technique. The results showed that 99% lincomycin was degraded after composting. For both the lincomycin mycelia dreg compost and sewage sludge compost, the absolute total of ARGs was all increased. By contrast, the relative total of ARGs were all decreased by 5% and 22% respectively. IntI1 was enriched in lincomycin mycelia dreg compost indicating the potential ecological risk. The redundancy analysis revealed that the antibiotic resistance genes were greatly influenced by the environmental factors and the order was pH > licomycin residue > temperature > C/N.
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