Mechanism of long-term chromium stress on Shewanella oneidensis MR-1using whole genome resequencing technique
XIAO Chang-ye1,2, XIAO Yong1, ZHAO Feng1
1. Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Acaderny of Sciences, Xiamen 361021, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China
Abstract：The 120-day long-term stress of constant concentration of Cr(VI) and Cr(Ⅲ) on Shewanella oneidensis MR-1 was investigated in the present study. The reduction ability of Cr(VI) and growth were explored after the 120-day cultivation. The electrochemical properties were characterized, and the genetic variation of strains at different stages was analysed using whole genome resequencing technology. The results showed that the long-term stress of Cr(VI) significantly improved the tolerance and reducing ability of Cr(VI). The initial Cr(VI) concentration of 55mg/L was completely reduced within 38h after the 120-day cultivation. There was a significant difference in the binding of cytochrome c to riboflavin near the -0.2V reduction peak between the strains cultured in media with Cr(VI) and Cr(Ⅲ). The mutation gene numbers of strains under long-term Cr(VI) stress were significantly higher than that in Cr(Ⅲ) environment. Non-synonymous mutated genes in strains under Cr(Ⅲ) stress mainly involved in glucose transformation and synthesis, genes encoding respiratory chain enzymes and synthetic ATPase, genes encoding signal transduction and transmembrane transporters. After the 120-day of Cr (VI) stress, mutated genes in strains mainly involved with cell membrane component genes, transporters, signal transduction genes, DNA synthesis repair genes and redox activity.
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