厌氧氨氧化颗粒污泥胞外聚合物金属元素特性

杨明明; 党超军; 张爱余; 王晗; 张夏武; 郭劲松; 方芳

PDF(885 KB)

中国环境科学 ›› 2020, Vol. 40 ›› Issue (11) : 4728-4734.

水污染与控制

厌氧氨氧化颗粒污泥胞外聚合物金属元素特性

杨明明^1,2, 党超军², 张爱余¹, 王晗³, 张夏武², 郭劲松¹, 方芳¹

作者信息 +

Characteristics of metal elements in anammox granular sludge extracellular polymeric substances

YANG Ming-ming^1,2, DANG Chao-jun², ZHANG Ai-yu¹, WANG Han³, ZHANG Xia-wu², GUO Jing-song¹, FANG Fang¹

Author information +

文章历史 +

摘要

为探究厌氧氨氧化颗粒污泥胞外金属元素特性，将厌氧氨氧化颗粒污泥根据粒径筛分为0.5~1.4mm、1.4~2.8mm、>2.8mm 3组，提取不同粒径厌氧氨氧化颗粒污泥胞外聚合物（EPS），研究EPS金属元素特性.结果表明，蛋白质（PN）是厌氧氨氧化颗粒污泥EPS的主要成分，占EPS含量的84.2%以上.随着粒径的增大，EPS中Na、K、Ca、Mg元素含量均增多，且与EPS中蛋白质含量变化一致.EPS中K、Ca、Mg元素的离子形式占比分别为68.6%、56.2%、94.7%.EPS经过阳离子交换树脂（CER）处理后，0.5~1.4mm、1.4~2.8mm、>2.8mm组EPS Zeta电位分别减小了4.7，7.2，9.1mV，EPS中的金属离子可通过压缩双电层作用促进颗粒污泥的聚集，金属离子对大粒径颗粒污泥EPS Zeta电位的影响幅度更大.

Abstract

In order to explore the impacts of extracellular metal elements on anammox granular sludge, anammox granular sludge was divided into three groups based on its sizes (0.5~1.4mm,1.4~2.8mm and>2.8mm). Then, the extracellular polymeric substrances (EPS) of the anxmmox granular sludge were extracted. Results show that proteins (PN) accounted for more than 84.2% of the EPS content, showing that PN were the main components of EPS. The anammox granular sludge EPS mainly contained four metal elements, K, Na, Ca, and Mg. In addition, above four metals were arranged in a descending order based on their concentration. With the increase in particle size, more metals and PN were found in EPS. The ionic forms of K, Ca, and Mg in the EPS accounted for 68.6%, 56.2%, and 94.7%, respectively. When the EPS were treated with cation exchange resin, the Zeta potential of the EPS of the 0.5~1.4mm, 1.4~2.8mm, >2.8mm groups decreased by 4.7mV, 7.2mV, and 9.1mV, respectively. Furthermore, the metal ions in EPS could promote the aggregation of granular sludge by compressing the electric double layer, and the effect of metal ions was more significant on larger-size granular sludge in Zeta potential.

导出引用

杨明明, 党超军, 张爱余, 王晗, 张夏武, 郭劲松, 方芳. 厌氧氨氧化颗粒污泥胞外聚合物金属元素特性[J]. 中国环境科学. 2020, 40(11): 4728-4734

YANG Ming-ming, DANG Chao-jun, ZHANG Ai-yu, WANG Han, ZHANG Xia-wu, GUO Jing-song, FANG Fang. Characteristics of metal elements in anammox granular sludge extracellular polymeric substances[J]. China Environmental Science. 2020, 40(11): 4728-4734

中图分类号： X703

参考文献

[1] Jetten M S M, Wagner M, Fuerst J, et al. Microbiology and application of the anaerobic ammonium oxidation ('anammox') process[J]. Current Opinion in Biotechnology, 2001,12(3):283-288.
[2] Tomaszewski M, Cema G, Ziembinska-Buczynska A. Influence of temperature and pH on the anammox process:A review and meta-analysis[J]. Chemosphere, 2017,182:203-214.
[3] Strous M, Heijnen J J, Kuenen J G, et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J]. Applied Microbiology and Biotechnology, 1998,50(5):589-596.
[4] Ma B, Wang S, Cao S, et al. Biological nitrogen removal from sewage via anammox:Recent advances[J]. Bioresource Technology, 2016,200:981-990.
[5] Fang F, Yang M M, Wang H, et al. Effect of high salinity in wastewater on surface properties of anammox granular sludge[J]. Chemosphere, 2018,210:366-375.
[6] 唐崇俭,熊蕾,王云燕,等.高效厌氧氨氧化颗粒污泥的动力学特性[J]. 环境科学, 2013,34(9):3544-3551. Tang C J, Xiong L, Wang Y Y, et al. Kinetic characteristics of high-rate ANAMMOX Granules[J]. Environmental Science, 2013,34(9):3544-3551.
[7] Sheng G P, Yu H Q, Li X Y. Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems:A review[J]. Biotechnology Advances, 2010,28(6):882-894.
[8] 宋成康,王亚宜,韩海成,等.温度降低对厌氧氨氧化脱氮效能及污泥胞外聚合物的影响[J]. 中国环境科学, 2016,36(7):2006-2013. Song C K, Wang Y Y, Han H C, et al. Effect of decreasing temperature on the performance and extracellular polymer substance of anaerobic ammonia oxidation sludge[J]. China Environmental Science, 2016, 36(7):2006-2013.
[9] Sarma S J, Tay J H, Chu A. Finding knowledge gaps aerobic granulation technology[J]. Trends in Biotechnology, 2017,35(1):66-78.
[10] 林治岐,张信,邵尉,等.废水生物处理过程中微生物胞外聚合物与污染物的分子间相互作用[J]. 中国科学:化学, 2018,48(9):1102-1108. Lin Z Q, Zhang X, Shao W, et al. Intermolecular interaction between microbial extracellular polymeric substances (EPS) and contaminants during biological wastewater treatment process[J]. Science Sinica Chimica, 2018,48(9):1102-1108.
[11] D'abzac P, Bordas F, Joussein E, et al. Characterization of the mineral fraction associated to extracellular polymeric substances (EPS) in anaerobic granular sludges[J]. Environmental Science & Technology, 2010,44(1):412-418.
[12] D'abzac P, Bordas F, Joussein E, et al. Metal binding properties of extracellular polymeric substances extracted from anaerobic granular sludges[J]. Environmental Science and Pollution Research, 2013, 20(7):4509-4519.
[13] 杨新萍,韩娇,周立祥. Ca²⁺在好氧颗粒污泥形成中的作用[J]. 环境科学, 2010,31(5):1269-1273. Yang X P, Han J, Zhou L X. Role of Ca²⁺ in the formation of glucose-fed aerobic granular sludge in sequencing batch reactor[J]. Environmental Science, 2010,31(5):1269-1273.
[14] Yan L L, Liu Y, Wen Y, et al. Role and significance of extracellular polymeric substances from granular sludge for simultaneous removal of organic matter and ammonia nitrogen[J]. Bioresource Technology, 2015,179:460-466.
[15] Wang H, Li H X, Fang F, et al. Underlying mechanisms of ANAMMOX bacteria adaptation to salinity stress. Journal of industrial microbiology & biotechnology[J]. 2019,46(5):573-585.
[16] Van de Graaf A A, de Bruijn P, Robertson L A, et al. Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor[J]. Microbiology, 1996,142(8):2187-2196.
[17] Lotti T, Kleerebezem R, Lubello C, et al. Physiological and kinetic characterization of a suspended cell anammox culture[J]. Water Research, 2014,60:1-14.
[18] 杨明明,刘子涵,周杨,等.厌氧氨氧化颗粒污泥EPS及其对污泥表面特性的影响[J]. 环境科学, 2019,40(5):2341-2348. Yang M M, Liu Z H, Zhou Y, et al. Extracellular polymeric substances of anammox granular sludge and its effect on sludge surface characteristics[J]. Environmental Science, 2019,40(5):2341-2348.
[19] Jia F X, Yang Q, Liu X H, et al. Stratification of extracellular polymeric substances (EPS) for aggregated anammox microorganisms[J]. Environmental Science & Technology, 2017,51(6):3260-3268.
[20] Egli K, Fanger U, Alvarez P J J, et al. Enrichment and characterization of an anammox bacterium from a rotating biological contactor treating ammonium-rich leachate[J]. Archives of Microbiology, 2001,175(3):198-207.
[21] Hou X L, Liu S T, Zhang Z T. Role of extracellular polymeric substance in determining the high aggregation ability of anammox sludge[J]. Water Research, 2015,75:51-62.
[22] 张亚超,张晶,侯爱月,等.胞外聚合物和信号分子对厌氧氨氧化污泥活性的影响[J]. 中国环境科学, 2019,39(10):4133-4140. Zhang Y C, Zhang J, Hou A Y, et al. Effect of extracellular polymers and signal molecules on the activity of ANAMMOX sludge[J]. China Environmental Science, 2019,39(10):4133-4140.
[23] Wang B, Wu D, Ekama G A, et al. Characterization of a new continuous gas-mixing sulfidogenic anaerobic bioreactor:Hydrodynamics and sludge granulation[J]. Water Res., 2018,135:251-261.
[24] Li X M, Liu Q Q, Yang Q, et al. Enhanced aerobic sludge granulation in sequencing batch reactor by Mg²⁺ augmentation[J]. Bioresource Technology, 2009,100(1):64-67.
[25] Jiang H L, Tay J H, Liu Y, et al. Ca²⁺ augmentation for enhancement of aerobically grown microbial granules in sludge blanket reactors[J]. Biotechnology Letters, 2003,25(2):95-99.
[26] Pevere A, Guibaud G, Van Hullebusch E D, et al. Effect of Na⁺ and Ca²⁺ on the aggregation properties of sieved anaerobic granular sludge[J]. Colloids and Surfaces a-Physicochemical and Engineering Aspects, 2007,306(1-3):142-149.
[27] Li W W, Yu H Q. Insight into the roles of microbial extracellular polymer substances in metal biosorption[J]. Bioresource Technology, 2014,160:15-23.
[28] 许冬冬,康达,郭磊艳,等.厌氧氨氧化颗粒污泥研究进展[J]. 微生物学通报, 2019,46(8):1653-1665. Xu D D, Kang D, Guo L Y, et al. Research progress on Anammox granular sludge[J]. Microbiology China, 2019,46(8):1653-1665.
[29] Sheng G P, Xu J, Li W H, et al. Quantification of the interactions between Ca²⁺, Hg²⁺ and extracellular polymeric substances (EPS) of sludge[J]. Chemosphere, 2013,93(7):1436-1441.
[30] Bourven I, Joussein E, Guibaud G. Characterisation of the mineral fraction in extracellular polymeric substances (EPS) from activated sludges extracted by eight different methods[J]. Bioresource Technology, 2011,102(14):7124-7130.
[31] Liu Y Q, Liu Y, Tay J H. The effects of extracellular polymeric substances on the formation and stability of biogranules[J]. Applied Microbiology and Biotechnology, 2004,65(2):143-148.
[32] Yu G H, He P J, Shao L M. Characteristics of extracellular polymeric substances (EPS) fractions from excess sludges and their effects on bioflocculability[J]. Bioresource Technology, 2009,100(13):3193-3198.
[33] Chen Z, Meng Y, Sheng B, et al. Linking exoproteome function and structure to anammox biofilm development[J]. Environmental Science & Technology, 2019,53(3):1490-1500.
[34] 魏亮亮,王胜,薛茂,等.城镇污泥胞外聚合物对重金属吸附特征及机制[J]. 哈尔滨工业大学学报, 2018,50(8):188-198. Wei L L, Wang S, Xue M, et al. Study on adsorption of heavy metals onto sludge extracellular polymers substances (EPS):A review[J]. Journal of Harbin Institute of Technology, 2018,50(8):188-198.
[35] Liu L, Gao D W, Zhang M, et al. Comparison of Ca²⁺ and Mg²⁺ enhancing aerobic granulation in SBR[J]. Journal of Hazardous Materials, 2010,181(1-3):382-387.
[36] Ren T T, Liu L, Sheng G P, et al. Calcium spatial distribution in aerobic granules and its effects on granule structure, strength and bioactivity[J]. Water Research, 2008,42(13):3343-3352.