Study on the denitrifying characteristics of oligotrophicaerobic denitrifying bacteria flora from source water reservoir
JIANG Yun-xia1,2, HUANG Ting-lin1,2, YANG Shang-ye1,2, KOU Li-qing1,2
1. Shannxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China;
2. Key Laboratory of Northwest Water Resource, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China
In order to reduce the nitrogen concentration in micro-polluted reservoirs, a mixed high efficient aerobic denitrifying flora-A1, dominated by Pseudomonas, was directional enrichment and domestication from the sediments of Lijiahe Reservoir (Xi'an). Shaker experiments showed that the nitrate nitrogen removal ratio of lean nutrient aerobic denitrifying flora A1reached 93.39% at 15h, and the average nitrate removal rate was 0.2073mg/ (L·h). The total nitrogen removal ratio was 52.11% and the average removal rate of total nitrogen was 0.1153mg/(L·h). There was no nitrite accumulation detected. Nitrogen balance analysis showed that about 45% of the initial nitrogen was removed and converted into gas products. Response surface method (RSM) results showed when C/N ratio=9.96, temperature=22.67 ℃, pH=8.01, speed=91r/min, dissolved oxygen=8.55mg/L, the optimum condition of total nitrogen (TN) removal could be obtained.
蒋云霞, 黄廷林, 杨尚业, 寇莉青. 水源水库贫营养好氧反硝化菌群脱氮特性研究[J]. 中国环境科学, 2020, 40(6): 2493-2502.
JIANG Yun-xia, HUANG Ting-lin, YANG Shang-ye, KOU Li-qing. Study on the denitrifying characteristics of oligotrophicaerobic denitrifying bacteria flora from source water reservoir. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(6): 2493-2502.
Huang T L, Guo L, Zhang H H, et al.Nitrogen-removal efficiency of a novel aerobic denitrifying bacterium, Pseudomonas stutzeri strain ZF31, isolated from a drinking-water reservoir [J].Bioresource Technology, 2015,196:209-216.
[2]
Fenech C, Rock L, Nolan K, et al. The potential for a suite of isotope and chemical markers to differentiate sources of nitrate contamination: a review [J].Water Research, 2012,46(7):2013-2041.
[3]
Wang Z, He S B, Huang J C, et al.Comparison of heterotrophic and autotrophic denitrification processes for nitrate removal from phosphorus-limited surface water [J].Environmental Pollution, 2018, 238:562-572.
[4]
Takenaka S, Zhou Q, Kuntiya A, et al.Isolation and characterization of thermotolerant bacterium utilizing ammonium and nitrate ions under aerobic conditions [J].Biotechnology Letters, 2007,29(3):385-390.
[5]
Robertson L A, Kuenen J G.Thiosphaerapantotrophagen.nov.sp.nov., a facultatively anaerobic, facultatively autotrophic sulphur bacterium [J].Microbiology.1983,129(9):2847-2855.
[6]
成钰,李秋芬,费聿涛,等.海水异养硝化-好氧反硝化芽孢杆菌SLWX2的筛选及脱氮特性[J].环境科学, 2016,37(7):2681-2688. Cheng Y, Li Q F, Fei Y T, et al.Screening and nitrogen removing characteristics of heterotrophic nitrification-aerobic denitrification bacteria SLWX2from sea water [J].Environmental Science, 2016, 37(7):2681-2688.
[7]
牟东阳,靳鹏飞,彭永臻,等.1株异养硝化-好氧反硝化细菌DK1的分离鉴定及其脱氮特性[J].环境科学, 2017,38(11):4763-4773. Mu D Y, Jin P F, Peng Y Z, et al.Identification and nitrogen removal characteristics of the heterotrophic nitrification and aerobic denitrification bacterial strain DK1[J].Environmental Science, 2017, 38(11):4763-4773.
[8]
杨静丹,祝铭韩,刘琳,等.异养硝化-好氧反硝化菌HY3-2的分离及脱氮特性[J].中国环境科学, 2020,40(1):294-304. Yang J D, Zhu M H, Liu L, et al.Isolation and denitrification characteristics of heterotrophic nitrification-aerobic denitrification bacterium HY3-2[J].China Environmental Science, 2020,40(1):294-304.
[9]
魏巍,黄廷林,苏俊峰,等.1株贫营养好氧反硝化菌的分离鉴定及其脱氮特性[J].生态环境学报, 2010,19(9):2166-2171. Wei W, Huang T L, Su J F, et al.Isolation and identification of an oligotrophic and aerobic denitrification and its denitrification characteristics [J].Ecology and Environmental Sciences, 2010,19(9): 2166-2171.
[10]
Huang T L, Zhou S L, Zhang H H, et al.Nitrogen removal from micro-polluted reservoir water by indigenous aerobic denitrifiers [J].International Journal of Molecular Sciences, 2015,16(2015):8008-8026.
[11]
Whiteley M, Diggle S P, Greenberg E P.Progress in and promise of bacterial quorum sensing research [J].Nature, 2017,551(7680):313-320.
[12]
Leinweber A, Fredrik I R, Kümmerli R.Cheating fosters species co-existence in well-mixed bacterial communities [J].Isme Journal, 2017,11(5):1179-1188.
[13]
Mujtaba G, Lee K.Treatment of real wastewater using co-culture of immobilized Chlorella vulgaris and suspended activated sludge [J].Water Research.2017,120:174-184.
[14]
雍佳君,成小英.蠡河底泥中反硝化复合菌群富集及菌群结构研究[J].环境科学, 2015,36(6):2232-2238. Yong J J, Cheng X Y.Enrichment and characterization of a denitrifying bacteria consortium from Lihe river’s sediment [J].Environmental Science, 2015,36(6):2232-2238.
[15]
康鹏亮,张海涵,黄廷林,等.湖库沉积物好氧反硝化菌群脱氮特性及种群结构[J].环境科学, 2018,39(5):2431-2437. Kang P L, Zhang H H, Huang T L, et al.Denitrification characteristics and community structure of aerobic denitrifiers from lake and reservoir sediments [J].Environmental Science, 2018,39(5):2431-2437.
[16]
Kuznetsov S, Dubinina G, Lapteva N.Biology of oligotrophic bacteria [J].Annual Reviews in Microbiology, 1979,33(1):377-387.
[17]
Wainwright M, Barakah F, Al-Turk I, et al.Oligotrophic micro-organisms in industry, medicine andthe environment [J].Science Progress, 1991,75(3/4(298)):313-322.
[18]
Button D, Schut F, Quang P, et al.Viability and isolation of marine bacteria by dilution culture: theory,procedures, and initial results [J].Applied and Environmental Microbiology, 1993,59(3):881-891.
[19]
Huang X F, Li W G, Zhang D Y, et al.Ammonium removal by a novel oligotrophic Acinetobacter sp.Y16capable of heterotrophic nitrification-aerobic denitrification at low temperature [J].Bioresource Technology, 2013,146:44-50.
[20]
周石磊,黄廷林,白士远,等.贫营养好氧反硝化菌的分离鉴定及其脱氮特性[J].中国环境科学, 2016,36(1):238-248. Zhou S L, Huang T L, Bai S Y, et al.Isolation, identification, and nitrogen removal characteristics of oligotrophic aerobic denitrifiers [J].China Environmental Science, 2016,36(1):238-248.
[21]
黄廷林,周娜,张海涵,等.3株贫营养好氧反硝化细菌的分离鉴定及反硝化特性[J].环境工程学报, 2014,8(12):5507-5513. Huang T L, Zhou N, Zhang H H, et al.Isolation and identification of three oligotrophic aerobic denitrifying bacteria and denitrification characteristics [J].Chinese Journal of Environmental Engineering, 2014,8(12):5507-5513.
[22]
张婷月,丁钰,黄民生.异养硝化-好氧反硝化细菌的筛选及其脱氮性能研究[J].华东师范大学学报(自然科学版), 2018,(6):22-31+87. Zhang T Y, Ding Y, Huang M S.Screening of heterotrophic nitrification-aerobic denitrifying bacteria and its nitrogen removal characteristics [J].Journal of East China Normal University (Natural Science), 2018,(6):22-31+87.
[23]
康鹏亮,陈胜男,黄廷林,等.好/厌氧条件下反硝化细菌脱氮特性与功能基因[J].环境科学, 2018,39(8):3789-3796. Kang P L, Chen S N, Huang T L, et al.Denitrification characteristics and functional genes of denitrifying bacteria under aerobic or anaerobic conditions [J].Environmental Science, 2018,39(8):3789-3796.
[24]
Throbäck I N, Enwall K, Jarvis A, et al.Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE [J].FEMS Microbiology Ecology, 2004,49(3):401-407.
[25]
Huang H K, Tseng S K.Nitrate reduction by Citrobacterdiversus under aerobic environment [J].Applied Microbiology & Biotechnology, 2001,55(1):90-94.
[26]
Tong J, Chen Y G.Recovery of nitrogen and phosphorus from alkaline fermentation liquid of waste activated sludge and application of the fermentation liquid to promote biological municipal wastewater treatment [J].Water Research, 2009,43(12):2969-2976.
[27]
国家环境保护总局.水和废水监测分析方法[M].第四版.北京:中国环境科学出版社, 2002:200-415. The State Environmental Protection Administration The Water and Wastewater Monitoring Analysis Method Editorial Board.Water and wastewater monitoring analysis method [M].4th Edition.Beijing: China Environmental Science Press, 2002:200-415.
[28]
杨霄.分层型水库水体细菌群落演变机制与扬水曝气强化作用研究[D].西安:西安建筑科技大学, 2016:49-64. Yang X.Study on the evolvement mechanism of aquatic bacteria community and its enhanced effect by water-liftling aerator in stratified reservoirs [D].Xi'an: Xi'an University of Architecture and Technology, 2016:49-64.
[29]
Ma F, Sun Y L, Li A, et al.Activation of accumulated nitrite reduction by immobilized Pseudomonas stutzeri T13 during aerobic denitrification [J].Bioresource Technology, 2015,187:30-36.
[30]
Zhang H H, Zhao Z F, Kang P L, et al.Biological nitrogen removal and metabolic characteristics of a novel aerobic denitrifying fungus Hanseniasporauvarumstrain KPL108[J].Bioresource Technology, 2018,267:569-577.
[31]
许涛,王国英,岳秀萍.Diaphorobacter sp.PDB3菌好氧反硝化脱氮特性[J].中国环境科学, 2018,38(6):2321-2328. Xu T, Wang G Y, Yue X P.The nitrogen removal characteristics of aerobic denitrification by Diaphorobactersp.PDB3[J].China Environmental Science, 2018,38(6):2321-2328.
[32]
Bai H, Liao S A, Wang A L, et al.High-efficiency inorganic nitrogen removal by newly isolated Pannonibacterphragmitetus B1[J].Bioresource Technology, 2018,271:91-99.
[33]
Ren Y X, Yang L, Liang X.The characteristics of a novel heterotrophic nitrifying and aerobic denitrifying bacterium, Acinetobacter juniiYB [J].Bioresource Technology, 2014,171:1-9.
[34]
Yao S, Ni J R, Ma T, et al.Heterotrophic nitrification and aerobic denitrification at low temperature by a newly isolated bacterium, Acinetobacter sp.HA2[J].Bioresource Technology, 2013,139:80-86.
[35]
颜薇芝,郝健,孙俊松,等.拉乌尔菌sari01的分离及其异养硝化好氧反硝化特性[J].环境科学, 2016,37(7):2673-2680. Yan W Z, Hao J, Sun J S, et al.Isolation of Raoultella sp.sari01 and its heterotrophic nitrification-aerobic denitrification characteristics [J].Huanjingkexue=Huanjingkexue, 2016,37(7):2673-2680.
[36]
王秀杰,王维奇,张阳,等.不同环境条件下Pseudomonas sp.脱氮特性及功能基因表达差异研究[J].中国环境科学, 2019,39(10): 4377-4386. Wang X J, Wang W Q, Zhang Y, et al.Effect of environmental factors on nitrogen removal and functional genes expression of strain Pseudomonassp [J].China Environmental Science, 2019,39(10): 4377-4386.
[37]
Marchant H K, Ahmerkamp S, Lavik G, et al.Denitrifying community in coastal sediments performs aerobic and anaerobic respiration simultaneously [J].The ISME journal, 2017,11(8):1799-1812.
[38]
Tong J, Chen Y G.Recovery of nitrogen and phosphorus from alkaline fermentation liquid of waste activated sludge and application of the fermentation liquid to promote biological municipal wastewater treatment [J].Water Research, 2009,43(12):2969-2976.
[39]
Mirazimi S M J, Rashchi F, Saba M.Vanadium removal from roasted LD converter slag: Optimization of parameters by response surface methodology (RSM) [J].Separation & Purification Technology, 2013, 116(37):175-183.
[40]
崔庆安.基于支持向量回归机的复杂过程响应曲面法研究[D].天津:天津大学, 2007:93-106. Cui Q A.Research on complex process response surface method based on support vector regression [D].Tianjing: Tianjin University, 2007: 93-106.
[41]
Chen Q, Ni J R.Ammonium removal by Agrobacterium sp. LAD9capable of heterotrophic nitrification―aerobic denitrification [J].Journal of Bioscience & Bioengineering, 2012,113(5):619-623.
[42]
Guo L J, Zhao B, An Q, et al.Characteristics of a novel aerobic denitrifying bacterium, Enterobacter cloacaeStrain HNR [J].Applied Biochemistry and Biotechnology, 2016,178(5):947-959.
[43]
郭琳.水源水库中好氧反硝化菌的筛选及脱氮性能研究[D].西安:西安建筑科技大学, 2015:66-67. Guo L.Study on the screening of aerobic denitrifiers from source water reservoir and the corresponding denitrification performance [D].Xi'an: Xi'an University of Architecture and Technology, 2015:66-67.