PDF(619 KB)
PDF(619 KB)
PDF(619 KB)
UASB启动运行特征及互营丙酸氧化菌定量分析
The performance of UASB at startup and the quantitative analysis of syntrophic propionate-oxidizing bacteria
为揭示升流式厌氧污泥床（UASB）反应器启动运行效能与互营丙酸降解菌群数量之间的关系，以稀释的玉米淀粉生产废水为底物，考察了UASB启动期的运行特征，并采用实时荧光定量PCR技术（qPCR）分析了互营丙酸降解菌群（丙酸氧化菌和产甲烷菌）的演替规律.结果表明，在进水COD 2000mg/L和水力停留时间（HRT）24h条件下，经过38d的连续运行，COD去除率达到了91.9%.当HRT分阶段缩短至8h时，比产甲烷速率达到了315LCH4/（kg COD·d），且形成了沉降性能良好的颗粒污泥.qPCR检测结果表明，至少有5种已鉴定的丙酸氧化菌存在于UASB反应器中.Pelotomaculum propionicum为接种污泥中的主要丙酸氧化菌，约占检测到丙酸氧化菌总数的45.7%.它的数量随着HRT缩短不断减少.而Syntrophobacter sulfatireducens和S.wolinii的数量随着HRT缩短不断增加，并在启动完成时达到最大值，分别为1.3×103，5.5×103个16S rRNA基因拷贝数/ng DNA，演替成为成熟颗粒污泥中的优势丙酸氧化菌群.Methanobacterium和Methanosarcina为接种污泥中的主要氢营养型产甲烷菌和乙酸营养型产甲烷菌，其数量随着HRT的缩短而逐渐减少，而Methanospirillum和Methanosaeta的数量随HRT的缩短逐渐增加，成为成熟颗粒污泥中的优势产甲烷菌群.
To reveal the relationship between the performance of upflow anaerobic sludge bed (UASB) reactor and syntrophic propionate-degrading consortia at startup, the operational characteristics during corn starch processing wastewater treatment in a UASB were investigated. The succession of syntrophic propionate-degrading consortia (propionate-oxidizing bacteria and methanogens) at startup was analyzed by quantitative real-time fluorescence polymerase chain reaction (qPCR). COD removal reached 91.9% after 38d operation under influent COD of 2000mg/L and hydraulic retention time (HRT) of 24h conditions. When HRT was shorten to 8h by stepwise, the specific methanogenic rate achieved 315LCH4/(kg COD·d) and the granular sludge with good settling performance was formed. qPCR analysis showed that five identified species of propionate propionate-oxidizing bacteria existed in the UASB reactor. Pelotomaculum propionicum was the major propionate-oxidizing bacteria in inoculation sludge, accounting for 45.7% in detectable propionate-oxidizing bacteria. Its number was reduced as HRT shorting. However, the quantity of Syntrophobacter sulfatireducens and S. wolinii were increased as HRT decrease. The maximum with 1.3×103 and 5.5×103 16S rDNA copies/ng DNA was reached in the end of startup, becoming the dominant propionate-oxidizing bacteria in the granular sludge. Methanobacterium and Methanosarcina were the dominant hydrogenotrophic and acetotrophic methanogens in the inoculation sludge. Their numbers were gradually reduced as HRT decrease. The quantity of Methanospirillum and Methanosaeta were increased, being the predominant methanogens in the granular sludge.
丙酸氧化菌 / 产甲烷菌 / 升流式厌氧污泥床 / 荧光定量PCR / 玉米淀粉生产废水
corn starch processing wastewater / methnaogens / propionate-oxidizing bacteria / qPCR / upflow anaerobic sludge bed
[1] 樊雅欣,刘红燕,潘凌峰,等.活化方式对过硫酸盐强化剩余污泥发酵的影响[J]. 中国环境科学, 2019,39(6):2460-2466. Fan Y X, Liu H Y, Pan L F, et al. Enhancement of waste activated sludge acidification by persulfate:Role of activation methods[J]. China Environmental Sciencece, 2019,39(6):2460-2466.
[2] 杨敏,陈德珍,戴晓虎.污泥热解液与牛粪混合厌氧消化特性研究[J]. 中国环境科学, 2018,38(2):634-642. Yang M, Chen D Z, Dai X H. Anaerobic co-digestion of the sewage sludge pyrolysis liquids and cow dung under thermophilic condition[J]. China Environmental Science, 2018,38(2):634-642.
[3] Antwi P, Li J, Boadi P O, et al. Functional bacterial and archaeal diversity revealed by 16S rRNA gene pyrosequencing during potato starch processing wastewater treatment in an UASB[J]. Bioresource Technology, 2017,235:348-357.
[4] Narihiro T, Terada T, Ohashi A, et al. Quantitative detection of previously characterized syntrophic bacteria in anaerobic wastewater treatment systems by sequence-specific rRNA cleavage method[J]. Water Research, 2012,46:2167-2175.
[5] 张立国,李彦霖,班巧英,等.qPCR揭示丙酸降解菌群随OLR提高的演替规律[J]. 中国环境科学, 2018,38(8):2997-3002. Zhang L G, Li Y L, Ban Q Y, et al. qPCR reveals the succession of syntrophic propionate-degrading consortia with OLR increase[J]. China Environmental Science, 2018,38(8):2997-3002.
[6] Li J, Ban Q, Zhang L, et al. Syntrophic propionate degradation in anaerobic digestion:A review[J]. International Journal of Agriculture and Biology, 2012,5:843-850.
[7] Lueders T, Pommerenke B, Friedrich M W. Stable-isotope probing of microorganisms thriving at thermodynamic limits:syntrophic propionate oxidation in flooded soil[J]. Applied and Environmental Microbiology, 2004,70:5778-5786.
[8] 班巧英,张瑞,张立国,等.荧光定量PCR解析酸性条件下丙酸氧化菌的演替[J]. 中国环境科学, 2017,37(10):3861-3866. Ban Q Y, Zhang R, Zhang L G, et al. RT-PCR analyze the succ-ession of propionate-oxidizing bacteria at acidic conditions in an UASB[J]. China Environmental Science, 2017,37(10):3861-3866.
[9] Ban Q, Li J, Zhang L, et al. Quantitative analysis of previously identified propionate-oxidizing bacteria and methanogens at different temperatures in an UASB reactor containing propionate as a sole carbon source[J]. Applied Biochemistry and Biotechnology, 2013, 171:2129-2141.
[10] Ariesyady H D, Ito T, Okabe S. Functional bacterial and archaeal community structures of major trophic groups in a full-scale anaerobic sludge[J]. Water Research, 2007,41:1554-1568.
[11] Ahlert S, Zimmermann R, Ebling J, et al. Analysis of propionate-degrading consortia from agricultural biogas plants[J]. Microbiology Open, 2016,5:1027-1037.
[12] Ban Q, Li J, Zhang L, et al. Syntrophic propionate degradation response to temperature decrease and microbial community shift in an UASB Reactor[J]. Journal of Microbiology and Biotechnology, 2013, 23:382-389.
[13] 国家环保局.水和废水监测分析方法[M]. 北京:中国环境科学出版社, 2002. State Environmental Protection Administration. Water and wastewater monitoring and analysis methods[M]. Beijing:China Environmental Science Press, 2002.
[14] Zhang Y, Wang X, Hu M, et al. Effect of hydraulic retention time (HRT) on the biodegradation of trichloroethylene wastewater and anaerobic bacterial community in the UASB reactor[J]. Applied Microbiology and Biotechnology, 2015,99:1977-1987.
[15] 李亚峰,陈涛,闫旭.UASB反应器处理淀粉废水的效果及影响因素[J]. 工业安全与环保, 2010,365(5):1-2. Li Y F, Chen T, Yan X. The effect and influential factors of the UASB reactor treating starch wastewater[J]. Industrial Safety and Environmental Protection, 2010,365(5):1-2.
[16] 石宪奎. UASB反应器处理玉米淀粉废水稳定性研究[J]. 工业水处理, 2007,27(5):31-33. Shi X K. Study on operating stability of UASB reactor for treating corn starch process wastewater[J]. Industrial Water Treatment, 2007, 27(5):31-33.
[17] 田文超,付秋爽,姬志辉,等.UASB-好氧工艺处理玉米淀粉废水研究[J]. 上海环境科学, 2017,36(3):120-123. Tian WC, Fu QS, Ji ZH, et al. A study on the treatment of cornstarch wastewater by UASB-aerobic process[J]. Shanghai Environmental Sciences, 2017,36(3):120-123.
[18] Imachi H S, Sakai Ohashi A, Harada H, et al. Pelotomaculum propionicicum sp. nov., an, mesophilic, obligately syntrophic, propionate-oxidizing bacterium[J]. International Journal of Systematic and Evolutionary Microbiology, 2007,57:1487-1492.
[19] de Bok F A M, Harmsen H J M, Plugge C M, et al. The first true obligately syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp. nov., co-cultured with Methanospirillum hungatei, and emended description of the genus Pelotomaculum[J]. International Journal of Systematic and Evolutionary Microbiology, 2005,55:1697-1703.
[20] Ariesyady H D, Ito T, Okabe S. Functional bacterial and archaeal community structures of major trophic groups in a full-scale anaerobic sludge[J]. Water Research, 2007,41:1554-1568.
[21] Harmsen H J M, Kengen H M P, Akkermans A D L, et al. Detection and localization of syntrophic propionate-oxidizing bacteria in granular sludge by in situ hybridization using 16S rRNA-based oligonucleotide probes[J]. Applied and Environmental Microbiology, 1996,62:1656-1663.
[22] Zhang L, Ban Q, Li J. Microbial community dynamics at high organic loading rates revealed by pyrosequencing during sugar refinery wastewater treatment in a UASB reactor[J]. Frontiers of Environmental Science and Engineering, 2018,12(4):1-12.
[23] Worm P, Fermoso F G, Lens P N L, et al. Decreased activity of a propionate degrading community in a UASB reactor fed with synthetic medium without molybdenum, tungsten and selenium[J]. Enzyme and Microbial Technology, 2009,45:139-145.
[24] Shigematsu T, Era S, Mizuno Y, et al. Microbial community of a mesophilic propionate-degrading methanogenic consortium in chemostat cultivation analyzed based on 16S rRNA and acetate kinase genes[J]. Applied and Environmental Microbiology, 2006,72:401-415.
[25] Ban Q, Zhang L, Li J. Functional bacterial and archaeal dynamics at slightly acidic conditions revealed by pyrosequencing in a propionate-fed UASB reactor[J]. Desalination and Water Treatment, 2019,160:125-134.
[26] Liu Y, Whitman W B. Metabolic, phylogenetic, and ecological diversity of the methanogenic archaea[J]. Annals of New York of Academy Science, 2008,1125:171-189.
[27] Ban Q, Xing Y, Li J. et al. Stable performance and microbial community dynamics under low HRT conditions in a propionate-fed UASB reactor[J]. Desalination and Water Treatment, 2018,108:136-143.
国家自然科学基金资助项目（51708341）；山西省“青年三晋学者”支持计划；山西省青年科技研究基金资助项目（201701D221215）
京公网安备 11010802030352号 /
| 〈 |
|
〉 |
