Effects of humic acids on the adsorption, chemical speciation, and bioaccessibility of soil lead and cadmium
LUO Mei, BAI Hong-cheng, CHEN Ting-yue, WEI Shi-qiang
State Cultivation Base of Eco-agriculture for Southwest Mountainous Land, Chongqing Key Laboratory of Agricultural Resources and Environment, College of Resources and Environment, Southwest University, Chongqing 400716, China
Effects of humic acids (HAs) on the environmental behaviors of heavy metals in soil and their bioavailability are closely related to the molecular weight of HAs components. Three in vitro methods, i.e., PBET, SBRC and IVG, were adopted to explore the effects of different molecular weight components of HAs (F1:<5kDa, F2:5~10kDa, F3:10~30kDa, F4:>30kDa) on the bioaccessibility of soil cadmium (Cd) and lead (Pb), and the bioaccessibility of soil Cd and Pb in relation to their adsorption, transformation in soils was analyzed in combination with batch adsorption experiment and fractionation by continuous extraction. The results showed that:Soil adsorption capacity of Cd and Pb was affected not only by the amount of HAs added, but also by the molecular weight of HAs. F1fraction (HAs component with molecular weight < 5kDa) promoted the adsorption of Cd and Pb in soil, and the promoting effect increased with the increase of HAs added at the dosage of 0.2% C~1% C, while other fractions with molecular weight > 10kDa decreased the adsorption the capacity of Cd and Pb in soil. All fractions promoted the transformation of soil Cd and Pb from acid-extractable form to residue form regardless of the amounts of HAs added, and the promotion potential of low molecular weight components was stronger. Soil Pb2+ mainly existed as reducible form, accounting for 57%~89%. The proportion of acid-exchangeable Pb in soil gradually decreased with the increase of molecular weight of the HAs components added. The addition of HAs facilitated the transformation of soils Pb from active form to inactive form, thus reducing the bioactivity of Pb2+. The bioaccessibility of soil Cd and Pb in gastrointestinal stage was enhanced by the addition of fractions with molecular weight < 5k and 5~10k, whereas decreased by other fractions. The bioaccessibility of Cd and Pb in soil as influenced by different HAs fractions depended on their final existing forms when transformation reached to equilibrium:higher proportion of acid-exchangeable form meant a higher bioavailability, while the soil adsorption ability of heavy metals was less relevant to their bioavailability.
罗梅, 柏宏成, 陈亭悦, 魏世强. 腐殖酸对土壤铅镉吸附、赋存形态及生物可给性的影响[J]. 中国环境科学, 2020, 40(3): 1191-1202.
LUO Mei, BAI Hong-cheng, CHEN Ting-yue, WEI Shi-qiang. Effects of humic acids on the adsorption, chemical speciation, and bioaccessibility of soil lead and cadmium. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(3): 1191-1202.
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