基于碳源优化的反硝化除磷及微生物特性

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摘要接种厌氧/缺氧/好氧-生物接触氧化（AAO-BCO）系统的反硝化除磷污泥，采用厌氧/缺氧/好氧-序批式（AAO-SBR）系统，重点考察了乙酸盐和丙酸盐配比（1：0，2：1，1：1，1：2和0：1）对反硝化除磷效率的影响，同时通过高通量测序对比了不同配比下微生物菌群结构的变化.结果表明，5种工况下，AAO-SBR系统均具有较高的有机物去除和反硝化除磷能力.而当乙酸钠/丙酸钠=1：0时，厌氧阶段在高效利用COD（87.63%）的同时完成聚-β-羟基烷酸（PHAs）的合成（174mgCOD/gMLSS），释磷量高达31.22mg/L；缺氧阶段PO₄^3--P的去除（74%）伴随着NO₃^--N反硝化（90%），PHAs利用率为72.4%，实现了氮磷的高效去除.高通量测序结果表明：不同碳源配比影响了微生物菌群的丰富度和多样性，其中变形菌门（Proteobacteria，31%~76%）、绿弯菌门（Chloroflexi，1%~26%）、拟杆菌门（Bacteroidetes，2%~31%）等占据绝大比例，而乙酸钠、丙酸钠共存时，微生物的多样性较好.当乙酸钠为单一碳源时，系统中聚磷菌（PAOs，21.364%）在与聚糖菌（GAOs，2.317%）的竞争中占绝对优势.

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	潘婷
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	王一鑫
	於蒙

关键词 ： AAO-SBR反应器, 乙酸钠/丙酸钠, 反硝化除磷, 内碳源, 高通量测序

Abstract：The effect of sodium acetate/sodium propionate ratios (1:0, 2:1, 1:1, 1:2 and 0:1) on denitrifying phosphorus removal was investigated using anaerobic/anoxic/oxic-sequencing batch reactor (AAO-SBR) by seeding the activated sludge from anaerobic/anoxic/oxic-biological contact oxidation (AAO-BCO) system. The evolution of microbial community structure was also compared by high-throughput sequencing under different ratios. The results showed that the AAO-SBR system revealed high organic matter and denitrifying phosphorus removal abilities under five operating conditions. When the ratio of sodium acetate/sodium propionate was 1:0, COD utilization efficiency was 87.63% while poly β-hydroxyalkanoate (PHAs) of 174mgCOD/gMLSS was synthesized simultaneously in the anaerobic stage, and phosphorus release was up to 31.22mg/L. In the anoxic stage, PO₄^3--P (74%) was removed along with NO₃^--N denitrification (90%), and the utilization rate of PHAs was 72.4%, achieving efficient removals of nitrogen and phosphorus. High-throughput sequencing results showed that the abundance and diversity of microbial community were influenced by different carbon source ratios, among which Proteobacteria (31%~76%), Chloroflexi (1%~26%) and Bacteroidetes (2%~31%) occupied a large proportion. But the microbial diversity was higher when sodium acetate and sodium propionate were co-existed. Phosphate accumulating organisms (PAOs, 21.364%) were dominant with the competition of glycogen accumulating organisms (GAOs, 2.317%) using sodium acetate as the single carbon source.

Key words： AAO-SBR reactor sodium acetate/sodium propionate ratios denitrifying phosphorus removal internal carbon source high-throughput sequencing

收稿日期: 2019-11-14

PACS:

X703

基金资助:国家自然科学基金资助项目（51808482，51478410）；江苏省自然科学基金资助项目（BK20170506）；江苏省高等学校大学生创新训练计划项目（201811117079X）；国家博士后科学基金资助面上项目（2018M632392）

通讯作者: 张淼,讲师,006363@yzu.edu.cn E-mail: 006363@yzu.edu.cn

作者简介: 潘婷(1997-),女,山西阳泉人,扬州大学本科生.发表论文2篇.

引用本文:

潘婷, 张淼, 范亚骏, 刘义忠, 庞晶津, 王一鑫, 於蒙. 基于碳源优化的反硝化除磷及微生物特性[J]. 中国环境科学, 2020, 40(7): 2901-2908. PAN Ting, ZHANG Miao, FAN Ya-jun, LIU Yi-zhong, PANG Jing-jin, WANG Yi-xin, YU Meng. Denitrifying phosphorus removal and microbial characteristics based on the optimization of carbon sources. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(7): 2901-2908.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2020/V40/I7/2901

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