The spectral characteristics and biotoxicity of fractions obtained from two different DOM molecular weight fractionation methods
MA Xiao-yan1, CHEN Wen-feng1, CHENG Fang-de1, TANG Lei1, WANG Xiao-chang1, LIN Yu1, LIU Xiao-kun2
1. Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; 2. XI'AN Municipal Engineering Design & Research Institute CO. LTD., Xi'an 710068, China
Abstract：Dissolved organic matter (DOM) fractions with different molecular weights were prepared by ultrafiltration centrifuge tube and ultrafiltration cup, respectively. Under the two different DOM molecular weight fractionation methods, the characteristics of obtained fractions and their biotoxicity were explored, and their joint action mode were identified. DOM were obtained from municipal wastewater treatment plant effluent (defined as EfOM) and commercial natural humic acid (defined as SWR-NOM). The results showed that DOM fractions with different molecular weight obtained from the two fractionation methods had the same fluorescence peak. Humic-like substances were the main fluorescent dissolved organic matter (FDOM) in EfOM and SWR-NOM, accounting for 56% ~ 91% of FDOM. For the same DOM, the relative content of FDOM composition in different molecular weight components is basically consistent. The variation of SUVA254 and E2/E3 from different fractions obtained by ultrafiltration centrifuge tube was larger than that by ultrafiltration cup. For the luminescent bacteria acute toxicity, in the same molecular weight range, the luminescent bacteria toxicity value of DOM fractions obtained by ultrafiltration cup fractionation method was higher than that detected by ultrafiltration centrifuge tube fractionation method, but the acute toxicity of <1KDa fraction obtained from ultrafiltration cup fractionation method was lower than the limit of detection . Using the concentration addition model (CA) to judge the mode of joint action for each fraction in DOM, it was found that the joint toxicity of DOM mixture conforms to the CA model, and the predicted joint toxicity values account for 67% ~ 104% of detected toxicity value.
马晓妍, 陈文凤, 成芳德, 唐磊, 王晓昌, 林雨, 刘孝坤. 两种分子量分级下DOM的组分特征及其生物毒性[J]. 中国环境科学, 2021, 41(12): 5885-5895.
MA Xiao-yan, CHEN Wen-feng, CHENG Fang-de, TANG Lei, WANG Xiao-chang, LIN Yu, LIU Xiao-kun. The spectral characteristics and biotoxicity of fractions obtained from two different DOM molecular weight fractionation methods. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(12): 5885-5895.
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