垃圾渗滤液与再生水生物毒性的体外检测方法应用

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摘要本文总结了近年来在垃圾渗滤液和再生水生物毒性检测方面的主要体外试验模型和检测方法，并整理了这些模型和方法在生物毒性评价中的应用.目前常用的体外试验模型包括人源细胞系、其他哺乳动物细胞以及微生物细胞，相比较而言，人源细胞系在检测结果外推至人体时具有更强的说服力，因而其应用最为广泛.体外检测方法可概括为细胞毒性、遗传毒性和内分泌干扰效应检测三个方面，其中内分泌干扰效应的研究较多集中于雌激素效应.最后，本文提出开发三维体外细胞模型、体外试验与化学分析相结合、全面分析内分泌干扰效应和建立成组体外试验体系是渗滤液和再生水生物毒性检测方法的发展方向.

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	徐倩茹
	赵飞
	夏娜娜
	李欣慧
	高绪超
	杨永传
	毕学军

关键词 ：垃圾渗滤液, 再生水, 生物毒性, 体外试验模型, 体外检测方法

Abstract：Main in vitro models and in vitro assays for biotoxicity detection of landfill leachate and reclaimed water, and the application of these models and assays in the evaluation of biotoxicity were summarized. The commonly used in vitro models included human-derived cells, other mammalian cells, and microbial cells. In comparison, results obtained from human-derived cells were more persuasive when extrapolated to humans, which led to more widely application of human-derived cell models. One the other hand, in vitro assays included three aspects: detections of cell toxicity, genotoxicity, and endocrine disturbing effects. Among them, studies on endocrine disturbing effects focused more on estrogenic effects nowadays. Moreover, this paper proposed that the development of three-dimensional in vitro model, combination of in vitro assays with chemical analysis, more comprehensive analyses of endocrine disrupting effects, and establishment of group test system of in vitro assays could be the potential future directions for the development of biotoxicity assays of landfill leachate and reclaimed water.

Key words： landfill leachate reclaimed water biotoxicity in vitro models in vitro assays

收稿日期: 2020-02-09

PACS:

X171.5

基金资助:国家自然科学基金青年基金资助项目（21906089）

通讯作者: 毕学军,教授,xuejunb@126.com E-mail: xuejunb@126.com

作者简介: 徐倩茹(1996-),女,青岛理工大学硕士研究生,主要研究方向为水污染控制和水质生物毒性检测.

引用本文:

徐倩茹, 赵飞, 夏娜娜, 李欣慧, 高绪超, 杨永传, 毕学军. 垃圾渗滤液与再生水生物毒性的体外检测方法应用[J]. 中国环境科学, 2020, 40(9): 3887-3893. XU Qian-ru, ZHAO Fei, XIA Na-na, LI Xin-hui, GAO Xu-chao, YANG Yong-chuan, BI Xue-jun. Application of in vitro assays in the biotoxicity detection of landfill leachate and reclaimed water. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(9): 3887-3893.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2020/V40/I9/3887

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