武汉东湖沉积物好氧氨氧化微生物时空分布

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摘要采用实时荧光定量PCR （qPCR）技术，测定了武汉东湖沉积物中氨氧化古菌（AOA）和氨氧化细菌（AOB）氨单加氧酶基因（amoA）的丰度，并结合沉积物水体环境中各形态氮素的含量，分析氮素含量对AOA和AOB的时空分布的影响.结果显示，AOA amoA基因丰度大于AOB amoA基因丰度，表明AOA对氨氧化过程的贡献较大.同时，AOA和AOB amoA基因丰度都随深度增加而降低.此外，间隙水的总氮、氨氮、硝酸盐氮以及亚硝酸盐氮浓度分别为6.28~33.56、2.71~22.7、0.12~0.98、0.01~0.13mg/L；上覆水的总氮、氨氮、硝酸盐氮以及亚硝酸盐氮平均浓度分别为1.68，0.79，0.16，0.04mg/L；表层水的总氮、氨氮、硝酸盐氮以及亚硝酸盐氮平均浓度分别为1.34，0.62，0.11，0.03mg/L，表明东湖东湖沉积物相对于水体呈营养盐可释放状态.相关性分析表明：AOA amoA基因丰度与间隙水氨氮和亚硝酸盐氮浓度呈显著正相关（P<0.05），AOB amoA基因丰度与间隙水亚硝酸盐氮（NO₂^--N）浓度呈显著正相关（P<0.05）.

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关键词 ：实时荧光定量PCR, 沉积物, 氨氧化古菌, 氨氧化细菌, 丰度

Abstract：The amoA gene abundances of ammonia-oxidizing archaea (AOA-amoA) and bacteria (AOB-amoA) in the sediments of Lake Donghu, Wuhan, was determined by the real-time quantitative polymerase chain reaction (qPCR). Moreover, the influence of nitrogen content on the temporal and spatial distribution of AOA and AOB was analyzed. The results showed that the abundance of AOA-amoA was higher than that of AOB-amoA, indicating greater contribution of AOA-amoA to the ammonia oxidation process. Meanwhile, the abundances of AOA-amoA and AOB-amoA decreased with elevated sediment depth. In addition, the concentrations of total nitrogen (TN), ammonia nitrogen (NH₄⁺-N), nitrate nitrogen (NO₃^--N) and nitrite nitrogen (NO₂^--N) in interstitial water were 6.28~33.56, 2.71~22.7, 0.12~0.98 and 0.01~0.13mg/L, respectively. The average concentrations of TN, NH₄⁺-N, NO₃^--N and NO₂^--N in overlying water were 1.68, 0.79, 0.16 and 0.04mg/L, respectively, while in surface water were 1.34, 0.62, 0.11 and 0.03mg/L, respectively. These results suggested that the sediments of Lake Donghu were important sources of nutrient. In addition, the abundance of AOA-amoA was positively correlated with the concentrations of NH₄⁺-N and NO₂^--N in interstitial water (P<0.05), while the abundance of AOB-amoA was positively correlated with the concentration of NO₂^--N in interstitial water (P<0.05).

Key words： qPCR sediment ammoxidation archaea ammonia oxidizing bacteria abundance

收稿日期: 2020-08-24

PACS:

X524

基金资助:国家自然科学基金资助项目（51579092，51879099）；国家重点基础研究项目（2016YFC0401702）；广东省水利科技创新项目（2017—14）

通讯作者: 李祝,副教授,iamlizhu@163.com E-mail: iamlizhu@163.com

作者简介: 张志忠(1996-),男,湖北仙桃人,湖北工业大学硕士研究生,主要研究方向环境微生物生态.发表论文1篇.

引用本文:

张志忠, 程德玺, 廖明军, 汪淑廉, 李祝. 武汉东湖沉积物好氧氨氧化微生物时空分布[J]. 中国环境科学, 2021, 41(4): 1917-1924. ZHANG Zhi-zhong, CHENG De-xi, LIAO Ming-jun, WANG Shu-lian, LI Zhu. Spatiotemporal distribution of aerobic ammonia-oxidizing microorganisms in sediments of Lake Donghu, Wuhan. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(4): 1917-1924.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2021/V41/I4/1917

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