Influence of RSI-MO process on biogas desulfurization and analysis of microbial communities
RUAN Ren-jun1,2, LI Jia-le1, OU Kun-xuan1, ZHAO Chang-shuang1,2, SUN Jun-wei1, CAO Jia-shun2
1. School of Architecture and Civil Engineering, Anhui Polytechnic University, Wuhu 241000, China; 2. College of Environment, Hohai University, Nanjing 210098, China
Abstract:To achieve in-situ deep desulfurization of biogas derived from waste-activated sludge (WAS) anaerobic digestion, an integrated approach of optimal rusty scrap iron addition (20g/L) with micro-oxygen injection (RSI-MO) was constructed to disclose their combined influences on the performance of in-situ desulfurization. The operation of semi-continuous anaerobic/microaerobic reactor included seven stages (P1~P7). The operation of first stage (P1) was set as the control. The addition of RSI was started at the second stage (P2). The oxygen was introduced in P3 stage, and the concentrations were gradually increased from P3 to P7 stages. The results showed that MO induced the microbial sulfide oxidation by stimulating sulfur-oxidizing bacteria (SOB), and simultaneously promoted the chemical corrosion on iron to generate iron-sulfide precipitation. From P1 to P7, the synergistic effects of RSI and MO contributed to the hydrolytic-acidogenic efficiency. However, the overdose of oxygen inhibited the activities of methanogenic microorganisms, which caused the accumulation of VFAs. To demonstrated the combined effects of RSI and MO on the microbial diversity and abundance, the microbial communities in sludge samples of P1, P2 and P6 were analyzed via the high-throughput sequencing technology. The microbial analysis suggested that the RSI addition stimulated the activity of hydrogen-producing microorganisms (Syntrophobacter fumaroxidans), acetate-producing microorganisms (Roseomonas lacus, Sporomusa silvacetica) and methanogenic microorganisms (Methanoculleus palmolei, Methanolinea tarda, Methanosarcina mazei, Methanosaeta concilii, Methanococcus aeolicus) while the MO stimulated the activity of SOB for biological desulphurization enhancement.
阮仁俊, 李家乐, 欧坤轩, 赵昌爽, 孙俊伟, 操家顺. RSI-MO工艺对沼气脱硫的影响及微生物种群分析[J]. 中国环境科学, 2021, 41(4): 1909-1916.
RUAN Ren-jun, LI Jia-le, OU Kun-xuan, ZHAO Chang-shuang, SUN Jun-wei, CAO Jia-shun. Influence of RSI-MO process on biogas desulfurization and analysis of microbial communities. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(4): 1909-1916.
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