海水养殖底泥中外源汞甲基化及生物响应研究

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (470 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要本文选择沙蚕作为底栖生物，通过模拟海水养殖环境，研究在外源汞（Hg （NO₃）₂）胁迫下，沙蚕对汞（Hg）的甲基化和甲基汞（MeHg）的富集，以及沙蚕的生物应激响应.结果表明，海水养殖区沙蚕一方面自身具有将汞转化为MeHg的能力，同时沙蚕扰动会促进沉积物中Hg的甲基化，沙蚕扰动沉积物中MeHg的含量为无扰动沉积物中的1.93倍.随着外源汞含量和暴露时间的增加，沙蚕对MeHg的富集量逐渐增加，而富集速率逐渐降低.沙蚕体内MeHg富集含量为0.007~0.079mg/kg，占沙蚕体内总汞（THg）含量的31.20%~86.90%.与无机Hg相比，MeHg会对沙蚕造成更大的氧化压力，具有更强的生物毒性.沙蚕的SOD，CAT活性和GSH，MDA含量与暴露时间及含量有显著相关性.当沉积物中外源汞输入含量超过0.5mg/kg，沙蚕抗氧化应激系统将超过防御极限.

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	王书平
	宋玉梅
	刘爽
	郭鹏然

关键词 ：外源汞, 甲基化, 沉积物, 沙蚕, 氧化应激

Abstract：By simulation of mariculture environment and Nereis diversicolor as benthic, the methylation of Mercury (Hg), accumulation of methylmercury (MeHg) and the oxidative stress of Nereis diversicolor were studied under the existence of Hg(NO₃)₂. The results showed that mercury could be transformed to methylmercury by both Nereis diversicolor and the environment, especially in that with Nereis diversicolor. The concentration of MeHg in the sediments with Nereis diversicolor was 1.93 times that without Nereis diversicolor. With increasing of the external input mercury concentration and exposure time, the concentration of MeHg increased and the accumulation rate decreases gradually in benthic body. The concentration of methylmercury in Nereis diversicolor body was 0.007~0.079mg/kg that was 31.20%~86.90% of Total mercury. The activity of SOD and CAT, the concentration of GSH and MDA in the Nereis diversicolor were significantly correlated with exposed time and Hg concentration. Compared with inorganic Hg, Methylmercury could cause more oxidative stress and had stronger biotoxicity. The defense limit of the oxidative stress system of the silkworm would be broken, when the concentration of external input mercury exceeds 0.5mg/kg in the sediment.

Key words： exogenous mercury methylation sediment nereis diversicolor oxidative stress

收稿日期: 2020-11-02

PACS:	X503.23
	X55

基金资助:国家自然科学基金资助项目（21777150）；国家重点研发计划资助项目（2018YFC1801701）

通讯作者: 郭鹏然,研究员,prguo@fenxi.com.cn E-mail: prguo@fenxi.com.cn

作者简介: 王书平(1996-),男,湖北恩施人,昆明理工大学硕士研究生,主要从事固体废物资源化利用研究.发表论文2篇.

引用本文:

王书平, 宋玉梅, 刘爽, 郭鹏然. 海水养殖底泥中外源汞甲基化及生物响应研究[J]. 中国环境科学, 2021, 41(6): 2871-2880. WANG Shu-ping, SONG Yu-mei, LIU Shuang, GUO Peng-ran. Research of the methylation and biological response of exogenous mercury in mariculture sediments. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(6): 2871-2880.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2021/V41/I6/2871

[1]	黄洪辉,林钦,林燕棠,等.大亚湾网箱养殖海域大型底栖动物的时空变化[J]. 中国环境科学, 2005,25(4):412-416. Huang H H, Ling Q, Lin Y T, et al. Spatial-temporal variation of large macrobenthic animals in cage culture sea area in Daya Bay[J]. China Environmental Science, 2005,25(4):412-416.
[2]	Wang Y, Wei Y N, Guo P R, et al. Distribution variation of heavy metals in maricultural sediments and their enrichment, ecological risk and possible source-A case study from Zhelin bay in Southern China[J]. Marine Pollution Bulletin, 2016,113(1/2):240-246.
[3]	Wu Q H, Zhou H C, Tam N F Y, et al. Contamination, toxicity and speciation of heavy metals in an industrialized urban river:Implications for the dispersal of heavy metals[J]. Marine Pollution Bulletin, 2016,104(1/2):153-161.
[4]	Xu Z, Fan W, Shi Z, et al. Mercury and methylmercury bioaccumulation in a contaminated bay[J]. Marine Pollution Bulletin, 2019,143:134-139.
[5]	GB 18668-2002海洋沉积物质量[S].
[6]	GB 18668-2002 Marine sediment quality[S].
[7]	Wang R, Feng X B, Wang W X, In vivo mercury methylation and demethylation in freshwater tilapia quantified by mercury stable isotopes[J]. Environmental Science & Technology, 2013,47(14):7949-7957.
[8]	Helieen H K, Katarzyk K H, Zhang T, et al. Mechanisms regulating mercury bioavailability for methylating microorganisms in the aquatic environment:a critical review[J]. Environmental Science & Technology, 2013,47(6):2441-2456.
[9]	Celo V, Lean D R, Scott S L, Abiotic methylation of mercury in the aquatic environment, Science of the Total Environment[J], 2006, 368(1):126-137.
[10]	任家盈,姜霞,陈春霄,等.太湖营养状态对沉积物中总汞和甲基汞分布特征的影响[J]. 中国环境科学, 2013,33(7):1290-1297. Ren J Y, Jiang X, Cheng C X, et al. The Effect of nutrition status on sediments distribution characteristics of total mercury and methylmercury in Lake Taihu[J]. China Environmental Science, 2013, 33(7):1290-1297.
[11]	Ullrich S M, Tanton T W, Abdrashitova S A, Mercury in the Aquatic Environment:A Review of Factors Affecting Methylation[J]. Critical Reviews in Environmental Science and Technology, 2001,31(3):241-293.
[12]	Xu Z, Wu S, Christie P, et al. Impacts of estuarine dissolved organic matter and suspended particles from fish farming on the biogeochemical cycling of mercury in Zhoushan island, eastern China Sea[J]. Science of the Total Environment, 2020,705:135921.
[13]	Gothberg A, Greger M, Formation of methyl mercury in an aquatic macrophyte[J]. Chemosphere, 2006,65(11):2096-2105.
[14]	Rieder S R, Brunner I, Daniel O, et al. Methylation of mercury in earthworms and the effect of mercury on the associated bacterial communities[J]. PLOS One, 2013,8(4):e61215.
[15]	Patrick S, A review of the biology, ecology and potential use of the common ragworm Hediste diversicolor (O.F. Müller) (Annelida Polychaeta)[J]. Hydrobiologia, 2002,470(1-3):203-218.
[16]	Wang W X, Stupakoff I, Gagnon C, et al. Bioavailability of inorganic and methylmercury to a marine deposit-feeding polychaete[J]. Environmental Science & Technology, 1998,32(17):2564-2571.
[17]	Ernst G, Zimmermann S, Christie P, et al. Mercury, cadmium and lead concentrations in different ecophysiological groups of earthworms in forest soils[J]. Environmental Pollution, 2008,156(3):1304-1313.
[18]	Suthar S, Singh S, Dhawan S, Earthworms as bioindicator of metals (Zn, Fe, Mn, Cu, Pb and Cd) in soils:Is metal bioaccumulation affected by their ecological category?[J]. Ecological Engineering, 2008,32(2):99-107.
[19]	Francesco R, Winston W G, Quantification of Total Oxidant Scavenging Capacity of Antioxidants for Peroxynitrite, Peroxyl Radicals, and Hydroxyl Radicals[J]. Toxicology and Applied Pharmacology, 1999,156(2):96-105.
[20]	Jaramillo D M, Rocha A M, Chiang G, et al. Biochemical and behavioral responses in the estuarine polychaete Perinereis gualpensis (Nereididae) after in situ exposure to polluted sediments[J]. Ecotoxicology and Environmental Safety, 2013,89:182-188.
[21]	刘爽,马旭,刘宁,等.高效液相色谱-电感耦合等离子体质谱法测定海水养殖底泥中甲基汞和乙基汞[J]. 分析实验室, 2019, 38(9):1043-1047. Liu S, Ma X, Liu N, et al. Detection of methylmercury and ethylmercury in marine culture sediment by high performance liquid chromatography-inductively coupled plasma mass spectrometry[J]. Chinese Journal of Analysis Laboratory, 2019,38(9):1043-1047.
[22]	Wang M, Feng W, Shi J, et al. Development of a mild mercaptoethanol extraction method for determination of mercury species in biological samples by HPLC-ICP-MS[J]. Talanta, 2007,71(5):2034-2039.
[23]	Jia X Y, Gong D R, Han Y, et al. Fast speciation of mercury in seawater by short-column high-performance liquid chromatography hyphenated to inductively coupled plasma spectrometry after on-line cation exchange column preconcentration[J]. Talanta, 2012,88:724-729.
[24]	Cardoso P G, Lillebo A I, Pereira E, et al. Different mercury bioaccumulation kinetics by two macrobenthic species:the bivalve Scrobicularia plana and the polychaete Hediste diversicolor[J]. Marine Environmental Research, 2009,68(1):12-18.
[25]	Taylor V F, Bugge D, Jackson B P, et al. Pathways of CH3Hg and Hg ingestion in benthic organisms:an enriched isotope approach[J]. Environmental Science & Technology, 2014,48(9):5058-5065.
[26]	Canario J, Miguel C, Evidence for elevated production of methylmercury in salt marshes[J]. Environmental Science & Technology, 2007,41(21):7376-7382.
[27]	Kaschak E, Knopf B, Petersen J H, et al. Biotic methylation of mercury by intestinal and sulfate-reducing bacteria and their potential role in mercury accumulation in the tissue of the soil-living Eisenia foetida[J]. Soil Biology and Biochemistry, 2014,69:202-211.
[28]	Dang F, Zhao J, Greenfield B K, et al. Soil geochemistry and digestive solubilization control mercury bioaccumulation in the earthworm Pheretima guillemi[J]. Journal of Hazardous Materials, 2015,292:44-51.
[29]	Li M, Yang H, Gu J D, Phylogenetic diversity and axial distribution of microbes in the intestinal tract of the polychaete Neanthes glandicincta[J]. Microbial Ecology, 2009,58(4):892-902.
[30]	Sizmur T, Canario J, Edmonds S, et al. The polychaete worm Nereis diversicolor increases mercury lability and methylation in intertidal mudflats[J]. Environmental Toxicology and Chemistry, 2013,32(8):1888-1895.
[31]	Jonsson S, Skyllberg U, Nilsson M B, et al. Mercury methylation rates for geochemically relevant Hg(II) species in sediments[J]. Environmental Science & Technology, 2012,46(21):11653-11659.
[32]	Zhao L, Wang R, Zhang C, et al. Geochemical controls on the distribution of mercury and methylmercury in sediments of the coastal East China Sea[J]. Science of the Total Environment, 2019,667:133-141.
[33]	Ding L, Zhao K, Zhang L, et al. Distribution and speciation of mercury affected by humic acid in mariculture sites at the Pearl River estuary[J]. Environmental Pollution, 2018,240:623-629.
[34]	Hammerschmidt C R, Fitzgerald W F., Geochemical controls on the production and distribution of methylmercury in near-shore marine sediments[J]. Environmental Science & Technology, 2004,38(5):1487-1495.
[35]	Maity S, Banerjee R, Goswami P, et al. Oxidative stress responses of two different ecophysiological species of earthworms (Eutyphoeus waltoni and Eisenia fetida) exposed to Cd-contaminated soil[J]. Chemosphere, 2018,203:307-317.
[36]	Liu T, Wang X, Xu J, et al. Biochemical and genetic toxicity of dinotefuran on earthworms (Eisenia fetida)[J]. Chemosphere, 2017, 176:156-164.
[37]	Jonas N, Elias S J A, Reactive oxygen species, antioxidants, and the mammalian thioredoxin system[J]. Free Radical Biology and Medicine, 2001,31(11):1287-1312.
[38]	Regoli F, Giuliani M E, Oxidative pathways of chemical toxicity and oxidative stress biomarkers in marine organisms[J]. Marine Environmental Research, 2014,93(SI):106-117.
[39]	Beyersmann D, Hechtenberg S, Cadmium, Gene Regulation, and Cellular Signalling in Mammalian Cells[J]. Toxicology and Applied Pharmacology, 1997,144(2):247-261.
[40]	Rubino F M, Toxicity of Glutathione-Binding Metals:A Review of Targets and Mechanisms[J]. Toxics, 2015,3(1):20-62.
[41]	Moltedo G, Martuccio G, Catalano B, et al. Biological responses of the polychaete Hediste diversicolor (O.F.Muller, 1776) to inorganic mercury exposure:A multimarker approach[J]. Chemosphere, 2019, 219:989-996.
[42]	Sun F H, Zhou Q X, Oxidative stress biomarkers of the polychaete Nereis diversicolor exposed to cadmium and petroleum hydrocarbons[J]. Ecotoxicology and Environmental Safety, 2008,70(1):106-114.
[43]	Geracitano L A, Bocchetti R, Monserrat J M, et al. Oxidative stress responses in two populations of Laeonereis acuta (Polychaeta, Nereididae) after acute and chronic exposure to copper[J]. Marine Environmental Research, 2004,58(1):1-17.