Abstract：Two electron transport inhibitors (rotenone and anti-mycin A) were introduced to phosphine bio-purification systems, then comparative experiments were conducted to investigate the variations of the microbial growth and metabolisms, phosphorus transport and transformation, the production of reactive oxygen species (ROS), and oxidase activities. When rotenone and anti-mycin A were respectively added to R1 and R2 bioreactors, the average O2-· content in these two systems increased to 0.68 and 0.96 mmol/g during the operation time of 14~17 days, which was significantly higher than control group (i.e. R0 reactor). Besides O2-·, another kind of reactive oxygen species, i.e. HO·, was also detected in bio-purification systems. In addition, due to the ROS accumulation, the malondialdehyde (MDA) content in microorganisms kept at a high level, caused the purification efficiency of phosphine less than 75%. The average phosphine removal efficiency in the R1 and R2 bioreactors decreased to 65.1% and 59.5% respectively, after 15~17 days operation. The factors, e.g., pH, ROS, and oxidase activity, had significant effects on phosphine biological purification. As metal cations such as Mg2+ and Cu2+ migrated into the microbial cells, the enzyme activities of superoxide dismutase and catalase were dramatically improved, which led to the alleviation of the oxidative stress derived from the reactive species such as O2-· and HO·. As the consequence, the purification efficacy of phosphine was improved moderately.
Fan Y, Lv M, Niu X J, et al. The key step of gaseous phosphorus release in anaerobic digestion[J]. Process Safety and Environmental Protection, 2020,137:238-245.
[2]
王强,耿金菊,金红梅,等.太湖沉积物中微生物和磷化氢的时空分布及关系[J]. 中国环境科学, 2006,(3):350-354. Wang Q, Geng J J, Jin H M, et al. Temporal and spatial distributions of microbes and phosphine in Lake Taihu sediments[J]. China Environmental Science, 2006,(3):350-354.
[3]
邓菁.微生物法去除一氧化碳气中磷化氢的研究[D]. 昆明:昆明理工大学, 2013. Deng Q. The study biological removal the PH3 in the carbon monoxide[D]. Kunming:Kunming University of Science and Technology, 2013.
[4]
肖瑢,刘树根,杨希,等.活性污泥体系中磷化氢生物降解特性[J]. 环境工程学报, 2018,12(3):855-862. Xiao R, Liu S G, Yang X, et al. Biodegradation characteristics of phosphine in activated sludge systerm[J]. Chinese Journal of Environmental Engineering, 2018,12(3):855-862.
[5]
刘树根,苏福家,李婷,等.生物滴滤法净化低浓度磷化氢及其微生物群落分析[J]. 环境工程学报, 2018,12(12):124-132. Liu S G, Su F J, Li T, et al. Purification of low concentration phosphine by bio-trickling filter system and analysis of microbial community[J]. Chinese Journal of Environmental Engineering, 2018,12(12):124-132.
[6]
倪建国,吴成强,朱润晔,等.生物滴滤塔反硝化净化NO废气的启动[J]. 中国环境科学, 2008,28(5):444-448. Ni J G, Wu C Q, Zhu R Y, et al. Study on start-up of biotrickling filter for nitric oxide denitrification[J]. China Environmental Science, 2008,28(5):444-448.
[7]
付梅.磷化氢对海洋微藻的影响及作用机制研究[D]. 北京:中国科学院, 2013. Fu M. The effect and mechanism of phosphine on marine algae[D]. Chinese Academy of Science, 2013.
[8]
汪丽军,刘涛,董书军,等.磷化氢熏蒸对桔小实蝇氧化代谢体系的影响研究[J]. 中国农学通报, 2013,29(33):351-357. Wang L J, Liu T, Dong S J, et al. The effect of phosphine fumigation on oxidative metabolism of bactrocera dorsalis hendel[J]. Chinese Agricultural Science Bulletin, 2013,29(33):351-357.
[9]
李丽,牛晓君,陆美青,等.环境中磷化氢对水稻根际环境与土壤有效磷的影响研究[J]. 环境科学学报, 2015,35(6):1851-1857. Li L, Miu X J, Lu M Q, et al. Effect of phosphine in the environment on rice rhizosphere and available phosphorus in soil[J]. Acta Scientiae Circumstantiae, 2015,35(6):1851-1857.
[10]
国家环境保护总局.水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社, 2002. SEPA(State Environmental Protection Administration). Methods for monitoring and analysis of water and wastewater (4th Edition)[M]. Beijing:China Environmental Press, 2002.
[11]
GB/T 23195-2008蜂花粉中过氧化氢酶的测定方法紫外分光光度法[S]. GB/T 23195-2008 Method for the determination of catalase in bee pollen-Ultaraviolet spectrophotometry[S].
[12]
Liu S G, Yang X, Yao X F. Impacts of ammonia nitrogen on autothermal thermophilic micro-aerobic digestion for sewage sludge treatment[J]. Chemosphere, 2018,213:268-275.
[13]
刘曼,李一兵,王彦斌,等.掺铜介孔碳活化过硫酸氢盐高效降解双酚A[J]. 中国环境科学, 2017,37(11):4151-4158. Liu M, Li Y B, Wang Y B, et al. Activation of peroxymonosulfate by copper doped ordered mesoporous carbon for efficient destruction of bisphenol A[J]. China Environmental Science, 2017,37(11):4151-4158.
[14]
张秋萍,吴霞红,郑剑恒,等.生物样本中丙二醛测定方法的研究进展[J]. 理化检验:化学分册, 2016,52(8):979-985. Zhang Q P, Wu X H, Zheng J H, et al. Progress of researches on methods for determination of malondialdehyde in biological samples[J]. Physical Testing and Chemical Analysis (Part B:Chemical Analysis), 2016,52(8):979-985.
[15]
邱小忠,欧阳钧,余磊,等.抗霉素A抑制PC12细胞线粒体COⅡ基因表[J]. 神经解剖学杂志, 2004,20(2):167-170. Qiu X Z, Ou Y J, Yu L, et al. Antimycin A inhibit the expressiion of mitochondrial COⅡ Gene in PC12 Cells[J]. Chinese Journal of Neuroanatomy, 2004,20(2):167-170.
[16]
陈莎,张翔,张舒越,等.抗霉素A直接刺激线粒体所引起的超氧阴离子含量和膜电位变化的单线粒体水平研究[J]. 厦门大学学报(自然科学版), 2013,52(4):525-530. Chen S, Zhang X, Zhang S Y, et al. Study the effect of antimycin A on superoxide anion production and mitochondrial membrane potential of isolated mitochondria at the single-mitochondrion level[J]. Journal of Xiamen University(Natural Science), 2013,52(4):525-530.
[17]
竺飞燕,张雄,王百辰,等.鱼藤酮诱导PC12细胞凋亡及线粒体膜电位变化[J]. 中国药理学通报, 2014,30(2):266-269. Zhu F Y, Zhang X, Wang B C, et al. Rotenone induces apoptosis of PC12 cells and alteration in mitochondrial membrane potential[J]. Chinese Pharmacological Bulletin, 2014,30(2):266-269.
[18]
邓勇,逯军.鱼藤酮致线粒体氧化损伤的作用和机制[J]. 中南大学学报(医学版), 2014,39(10):1093-1099. Deng Y, Lu J. Role of rotenone in mitochondrial oxidative damage and the underlying mechanisms[J]. Journal of Central South University (Medical Science), 2014,39(10):1093-1099.
[19]
Li T, Liu S G, Yao X F. Addition of reactive oxygen scavenger to enhance PH3biopurification:Process and mechanism[J]. Process Safety and Environmental Protection, 2020,142:118-125.
[20]
Khachatryan L, Dellinger B. Environmentally persistent free radicals (EPFRs)-2. Are free hydroxyl radicals generated in aqueous solutions?[J]. Environmental Science & Technology, 2011,45(21):9232-9239.
[21]
Ahamd S, Maryam P, Bahareh S Y, et al. Inhibition of glucose-6-phosphate dehydrogenase protects hepatocytes from aluminum phosphide-induced toxicity[J]. Pesticide Biochemistry & Physiology, 2017,143:141-146.
[22]
Wang Z, Gao M, Wang Z, et al. Effect of salinity on extracellular polymeric substances of activated sludge from an anoxic-aerobic sequencing batch reactor[J]. Chemosphere, 2013,93(11):2789-2795.
