Kinetic characteristics of functional bacteria in IFAS process for partial nitrification
ZHANG Kai, LI Jun, LIANG Dong-bo, HOU Lian-gang, ZHANG Jing, WANG Xiu-jie
The College of Architecture and Civil Engineering, Beijing University of Technology, National Engineering Laboratory of Urban Sewage Advanced Treatment and Resource Utilization Technology, Beijing 100124, China
The kinetic characteristics of aerobic ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) under different influent NH4+-N loading and free ammonia (FA) concentration were studied by biofilm-activated sludge composite process (IFAS). The contributions of different microbial aggregates (suspended sludge and attached biofilm) to the removal of NH4+-N were investigated, and the biological adsorption and biodegradation were analyzed quantitatively. The amount and spatial distribution of total bacteria, AOB, and NOB were observed by fluorescence in situ hybridization (FISH). The experimental results showed that with the increased of influent NH4+-N concentration, the effluent NO3--N concentration decreased and the NO2--N accumulated a lot. When the influent NH4+-N concentration was 480mg/L, the NH4+-N removal rate and the nitrite accumulation rate (NAR) were stable above 95% and 80%, respectively, while the FA increased from (2.77±0.07) mg/L to (16.35±0.3) mg/L, and the NAR increased from 9.42% to 83.31%, which achieved the inhibition of NOB. In the process of NH4+-N removal, biossorption and microbial degradation accounted for 3.4% and 88.1% of NH4+-N removal, respectively. The ratio of AOB in suspended sludge and biofilm increased from 27.4% and 10.3% to 41.3% and 18.1%, respectively, indicating that suspended sludge was more beneficial to enrichment of AOB than biofilm.
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