Inhibition and process mechanism of ammonia nitrogen and sulfate on anaerobic degradation of glutamate
CHEN Hong1,2, HU Ying-bing1,2, CHEN Chen3, WEI Yan-xiao1,2, WANG Hong1,2, HUANG Rong1,2, YU Guan-long1,2, YANG Min2
1. Engineering and Technical Center of Hunan Provincial Environmental Protection for River-Lake Dredging Pollution Control, School of Hydraulic Engineering, Changsha University of Science&Technology, Changsha 410114, China; 2. Key Laboratory of Dongting Lake Aquatic Eco-Environmental Control and Restoration of Hunan Province, Changsha 410114, China; 3. Changsha Social Work College, Changsha 410004, China
Abstract：To investigate the effect of ammonia nitrogen and sulfate in the influent on anaerobic degradation of glutamate under the optimal organic loading rate, a lab-scale up-flow anaerobic sludge blanket (UASB) reactor was continuously operated for 1119 days. The results showed that the maximal COD removal efficiency as high as (97.94±0.28)% was obtained at an organic loading rate of 8.0g COD/(L·d). When step-wise increasing the ammonia nitrogen concentration in the influent, the UASB reactor a small fluctuation on glutamate removal efficiency under low ammonia nitrogen concentrations (<1000mg/L), subsequently appeared a distinct decline in the COD removal and methane yield at 2000mg/L, and then the half-inhibitory concentration of ammonia nitrogen exhibited at 4000mg/L. When step-wise increasing sulfate concentration in the influent from 200mg/L to 4000mg/L, methane yield and free sulfide (FS) concentration in the mixture fallen into increasing and decreasing trends, respectively; but the COD removal efficiencies were generally in a high level (>90%). Both the ammonia nitrogen and sulfate in the influent could inhibit the glutamate degradation activity of methane-producing archaea (MPA) through dissociation equilibrium and biological reduction, respectively. Besides, FAN could freely diffuse into microbial cells resulting in proton imbalance and then excessive ATP consumption in the former. In contrast, substrate competitive inhibition was existed as for the reproduction of sulfate-reducing bacteria in the latter.
陈宏, 胡颖冰, 陈晨, 韦燕霄, 黄蓉, 王泓, 余关龙, 杨敏. 氨氮和硫酸盐对谷氨酸厌氧生物降解性能的抑制及机理[J]. 中国环境科学, 2020, 40(10): 4342-4347.
CHEN Hong, HU Ying-bing, CHEN Chen, WEI Yan-xiao, WANG Hong, HUANG Rong, YU Guan-long, YANG Min. Inhibition and process mechanism of ammonia nitrogen and sulfate on anaerobic degradation of glutamate. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(10): 4342-4347.
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