Abstract：A dye degrading bacteria named WYT was isolated from printing and dyeing activated sludge, and the key genes involved in the degradation and decolorization of VB4dye were investigated. The whole genome sequencing and homologous recombination were used to identify the possible key genes of dye metabolism. The gene was knocked out, meanwhile, the vector was constructed for gene complement, and a phenotypic verification experiment was designed to verify the decolorization effect of the gene. The results showed that the possible key gene, named DyP, belonged to type B of DyP eroxidase gene.The DyP knockout strain had no decolorization effect on VB4. The decolorization rate of VB4 was 96.04% in the complementary strain and 96.95% in the wild type, indicating the decolorization rate of VB4 in the complementary strain was restored, DyP gene was the key gene of VB4 degradation and decolorization.
马倩, 李海红, 王洋涛. 菌株WYT降解还原蓝4的关键基因[J]. 中国环境科学, 2021, 41(7): 3283-3290.
MA Qian, LI Hai-hong, WANG Yang-tao. The key genes involved in degradtion of vat blue 4 in bacterial strain WYT. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(7): 3283-3290.
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