Study on the high concentration oil-containing wastewater by biofilm treatment of P.aeruginosa strain NY3 and the characteristics of microbial community in biofilm
LIN Ying-ying, NIE Mai-qian, WANG Yan, HE Mei-li, LI Hong
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China
Single P. aeruginosa NY3 can grow and be immobilized on the surface of polyurethane foam carrier, the biofilm was used in an open system (non sterile conditions) to treat the oil-containing wastewater. The results showed that the biofilm can efficiently removeoil material of high concentrationoily wastewater. After continuously running for 25 d in the oil-bearing wastewater of 2g/L, the removal rate of petroleum hydrocarbons can be maintained at 91.1%. When the entrance of water was stopped, the bioactivityof the biofilm can be recovered by aeration. Under the natural environment, the bacterial accompanied and survived with NY3 in bacteria biofilmmainly includedBacillus, Pseudomonas and Rhodococcus strain. Two cultured strains were isolated and identified through the 16SrRNAsequence, which showed they belonged toRhodococcusand Bacillus separately, and they were also proved to have the capabilty to efficiently remove petroleum hydrocarbon.
林莹莹, 聂麦茜, 王琰, 贺美丽, 李虹. 生物膜处理高含油废水及膜表面微生物群落特性研究[J]. 中国环境科学, 2016, 36(9): 2800-2806.
LIN Ying-ying, NIE Mai-qian, WANG Yan, HE Mei-li, LI Hong. Study on the high concentration oil-containing wastewater by biofilm treatment of P.aeruginosa strain NY3 and the characteristics of microbial community in biofilm. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(9): 2800-2806.
Zhao X, Wang Y, Ye Z, et al. Oil field wastewater treatment in biological aerated filter by immobilized microorganisms[J]. Process Biochemistry, 2006,41(7):1475-1483.
Abalos A, Vinas M, Sabate J, et al. Enhanced biodegradation of Casablanca crude oil by a microbial consortium in presence of a rhamnolipid produced by Pseudomonas aeruginosa AT10[J]. Biodegradation, 2004,15(4):249-260.
PIEKARSKA K.Preliminary studies on application of immo-bilized microorganisms to petroleum hydrocarbons biodegradation[C]//EuropeanSymposium on environmental biotechnology, 2004.
[8]
Nie M, Yin X, Ren C, et al. Novel rhamnolipid biosurfactants produced by a polycyclic aromatic hydrocarbon-degrading bacterium Pseudomonas aeruginosa strain NY3[J]. Biotechnology advances, 2010,28(5):635-643.
[9]
Manohar S, Kim C, Karegoudar T. Enhanced degradation of naphthalene by immobilization of Pseudomonas sp. strain NGK1in polyurethane foam[J]. Applied Microbiology and Biotechnology, 2001,55(3):311-316.
Li P, Wang X, Stagnitti F, et al. Degradation of phenanthrene and pyrene in soil slurry reactors with immobilized bacteria Zoogloea sp[J]. Environmental engineering science, 2005,22(3):390-399.
[14]
Theron J, Cloete T E. Molecular techniques for determining microbial diversity and community structure in natural environments[J]. Critical reviews in microbiology, 2000,26(1):37-57.
[15]
Ferris M J, Muyzer G, Ward D M. Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community[J]. Applied and Environmental Microbiology, 1996,62(2):340-346.
Araya R, Tani K, Takagi T, et al. Bacterial activity and community composition in stream water and biofilm from an urban river determined by fluorescent in situ hybridization and DGGE analysis[J]. FEMS Microbiology Ecology, 2003,43(1):111-119.
[19]
Tiedje J M. Microbial diversity: of value to whom[J]. ASM News, 1994,60(10):524-525.