Abstract：Using the anoxic/aerobic intermittent operation mode, the influence of influent C/N ratios (C/N=5.0, 3.3, 2.5, 2.0) on nitrite (NO2-) accumulation and pollutant degradation in partial denitrification process was investigated, and the microbial diversity and functional bacteria evolution were explored with high-throughput sequencing. At the C/N ratio of 2.5, the system achieved the best treatment performance, and the effluent NO2- concentration was 27.18mg/L with the nitrite transformation ratio (NTR) of 67.96%. The analysis of the degradation rules of each pollutant in typical cycle revealed that the degradation rate of COD directly affected the process of denitrification and the stable NO2- accumulation can only be maintained under the condition of lower C/N ratios (C/N=2.0~2.5), although NO2-accumulation peaked at 30min of anoxic stage in all the four operations with the highest NO2- values of 4.86(C/N=5.0), 16.52(C/N=3.3), 30.16(C/N=2.5), and 20.28 (C/N=2.0) mg/L, respectively. High-throughput sequencing results showed that in addition to traditional functional bacteria of Thauera(2.67%~24.04%), Terrimonas(4.94%~21.19%) and Saprospiraceae(5.34%~13.50%), Flavobacterium(28.23%) was the dominant bacteria that maintained high NO2- accumulation when the C/N ratio was 2.5. Combined with the operational characteristics of partial denitrification process, the application feasibility of related coupling processes with NO2-as intermediate product was discussed.
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