Intermittent aeration-internal circulation biological filter performance and biofilm characteristics
REN Wu-ang1, CAO Feng-feng1, JU Kai1, JIN Peng-kang2, LI Si-min3, CHAI Bei-bei4,5, LEI Xiao-hui5
1. School of Architecture and Civil Engineering, Xi'an University of Science and Technology, Xi'an 710054, China; 2. School of Human Settlement Environment and Civil Engineering, Xi'an Jiaotong University, Xi'an 710049, China; 3. Hebei Water Pollution Control and Ecological Restoration Technology Innovation Center, Handan 056038, China; 4. School of Water Conservancy and Hydroelectric Power, Hebei University of Engineering, Handan 056038, China; 5. Hebei Key Laboratory of Intelligent Water Conservancy, Handan 056038, China
Abstract：The mechanism of enhanced denitrification by intermittent aeration-coupled internal circulation biofiltration is still not clear. We therefore investigated the pollutant removal in a biofiltration reactor. Several methods were used to analyze the biomass, biological activity, and nitrification and denitrification rates of the system, and the microbial population in the reactor was analyzed. The intensity of protein-like fluorescence gradually decreased throughout the system, and no protein-like peak was detected in the effluent. The decrease of NH4+-N along the 10-50cm section of the reactor did not cause a significant increase in NO3--N, and the NO3--N content decreased at 50cm. This section showed obvious denitrification. The dissolved oxygen and biomass levels throughout the system indicated an anoxic/anaerobic environment and significant denitrification. In addition, the low oxygen uptake rate (OUR) but high TTC-dehydrogenase activity at 50cm and the significantly higher denitrification rate compared to the nitrification rate indicated enhanced denitrification in this region. Based on 16S rRNA high-throughput sequencing analysis, the system had a higher abundance of microorganisms involved in denitrification at the phylum level, mainly Firmicutes (10.64%) and Bacteroidetes (22.29%). Denitrification was also evident at the genus level in Comamonas (3.11%) and Hydrogenophaga (2.43%). Our results suggest that the BAF system with intermittent aeration coupled with internal circulation enhanced denitrification in the bottom zone and thus improved the denitrification efficiency.
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