己酸菌富集及其利用餐厨垃圾产己酸的研究

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摘要采用批式发酵的方式,考察3种不同电子供体(乙醇、乳酸、乙醇和乳酸)对己酸菌的富集情况,进而选取产己酸最优的混合菌,探究在不外加电子供体情况下,餐厨垃圾厌氧发酵产己酸的性能.结果表明:当以乙醇和乳酸共同作为电子供体时,己酸浓度可达(2303.06±499.56) mgCOD/L,产己酸效果最佳,并显著富集到了3种碳链延长菌:Clostridium_sensu_stricto_12(16.04%±0.16%),Caproiciproducens(28.78%±0.15%),Oscillibacter(2.32%±0.03%);将产己酸最优的混合菌接种于餐厨垃圾进行厌氧发酵,己酸浓度最高可达(8657.36±996.21) mg COD/L,己酸产率为(230.05±26.47) mg COD/gVS,己酸选择度为27.74%±3.19%.以上结果验证了以乙醇和乳酸共同作为电子供体时对己酸菌具有明显的富集作用,富集到的混合菌可有效用于餐厨垃圾的产己酸发酵,为餐厨垃圾的资源化利用提供了理论支撑.

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	张艳艳
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关键词 ：电子供体, 己酸, 餐厨垃圾, 厌氧发酵

Abstract：This study investigated three different electron donor combinations to attain maximum enrichment of caproic acid bacteria and the ability of these enriched bacteria to further digest food waste without the addition of external electron donors. Three batch experiments were performed using ethanol, lactate, and ethanol plus lactate as electron donors. The results showed that the highest concentration of caproic acid ((2303.06±499.56) mg COD/L) was reached when ethanol and lactate were both used as electron donors which was justified by the significant increase in the microbial population of caproate bacterial genera such as Clostridium_sensu_stricto_12 (16.04%±0.16%), Caproiciproducens (28.78%±0.15%) and Oscillibacter (2.32%±0.03%). When this enriched culture was inoculated to food waste, the maximum concentration of caproate reached (8657.36±996.21) mg COD/L, and the caproic acid yield and specificity were (230.05±26.47) mg COD/g VS and 27.74%±3.19% respectively. This study proved that caproic acid bacteria are significantly enriched in the presence of the electron donor combination of ethanol and lactate, and that the enriched inoculum can promote caproic acid production from food waste. These results provide theoretical support for the recovery of resources from food waste.

Key words： electron donors caproic acid food waste anaerobic fermentation

收稿日期: 2021-12-01

PACS:

X705

基金资助:国家重点研发计划项目(2017ZX07103-007)

通讯作者: 左剑恶,教授,jiane.zuo@mail.tsinghua.edu.cn E-mail: jiane.zuo@mail.tsinghua.edu.cn

作者简介: 张艳艳(1991-),女,山东潍坊人,清华大学博士研究生,主要研究方向为餐厨垃圾厌氧发酵.发表论文1篇.

引用本文:

张艳艳, 白佳喆, 左剑恶. 己酸菌富集及其利用餐厨垃圾产己酸的研究[J]. 中国环境科学, 2022, 42(6): 2724-2733. ZHANG Yan-yan, BAI Jia-zhe, ZUO Jian-e. Enrichment of caproate bacteria and its application in caproic acid production from food waste. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(6): 2724-2733.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2022/V42/I6/2724

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