金属离子对青霉素菌渣厌氧发酵产气模型分析

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (933 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要为比较响应面法与反向传播神经网络法在厌氧发酵过程中的应用效果，以青霉素菌渣为原料，通过单因素和Box-Behnken法设计试验，在发酵体系中添加不同量的Fe²⁺、Co²⁺、Ni²⁺，以确定其对青霉素菌渣厌氧产气性能的影响.结果表明，Fe²⁺、Co²⁺、Ni²⁺单一最佳添加量为：500mg/L、30mg/L、0.3mg/L，产沼气量较对照分别提高了：102.18%、45.48%、60.12%.其促进作用随添加浓度增大呈现：弱-强-弱趋势.使用响应面法及反向传播神经网络法对金属离子添加量进行建模优化，并使用批式厌氧发酵进行验证.响应面法建模预测Fe²⁺、Co²⁺、Ni²⁺最佳混合添加浓度为：440.94mg/L、16.22mg/L、0.39mg/L，预测累积产沼气量为1314.49mL，R²=0.972，试验与验证相对误差为4.65%；反向传播神经网络法建模Fe²⁺、Co²⁺、Ni²⁺最佳混合添加浓度为495mg/L、21mg/L、0.5mg/L，预测产沼气量为1551.55mL，R²=0.991，试验与验证相对误差为0.47%.反向传播神经网络法建模具有更好的拟合效果且与验证试验误差小，是一种更有效的仿真方法.说明该方法在优化厌氧发酵金属离子添加具有应用潜力，同时也为厌氧发酵条件优化提供新思路.

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	方楠
	赵燕肖
	习彦花
	刘敬
	梁文华
	程辉彩
	张丽萍

关键词 ：青霉素菌渣, 厌氧发酵, 金属离子优化, 响应面法, 反向传播神经网络法

Abstract：In order to testify the element efficiency and compare the application of different models in anaerobic digestion of the penicillin fermentation residues, the optimized test was designed by Box-Behnken, modeled by the response surface methodology (RSM) and the backpropagation neural network (BP-ANN). Meanwhile, the optimal concentration of single and mixture was determined. The optimal concentrations of single trace elements of Fe²⁺, Co²⁺ and Ni²⁺ were 500mg/L, 30mg/L and 0.3mg/L, which the accumulated biogas production was increased by 102.18%, 45.48% and 60.12% compared with the control. The variation of gas promotion was "weaker-stronger-weaker" with the increasing concentration of different trace elements. The optimal mixture concentration of Fe²⁺, Co²⁺ and Ni²⁺ predicted by the RSM model were 440.94mg/L, 16.22mg/L and 0.39mg/L, R²=0.972, which the prediction of accumulated biogas production was 1314.49mL, and the experimental value was 1256mL with the RE (relative error)=4.65%. Meanwhile, the best-adding concentration of Fe²⁺, Co²⁺ and Ni²⁺ predicted by BP-ANN mode was 495mg/L, 21mg/and 0.5mg/L, R²=0.991, which prediction of accumulated biogas production was 1551.55mL and the experimental value was 1358mL with the RE=0.47%. Compared with the RSM model, the BP-ANN model had a better fit and robust prediction of adding trace elements in anaerobic digestion of the penicillin fermentation residues, which showed that the BP-ANN model had prudential application in the optimization of trace element in anaerobic digestion, and it also provides a new idea to the optimization of anaerobic digestion.

Key words： anaerobic digestion penicillin fermentation residues optimization of trace element the response surface methodology the backpropagation neural network

收稿日期: 2019-11-30

PACS:

X705

基金资助:河北省自然基金资助项目（C2020302004）；河北省高层次人才资助项目（E2015100006，A201802014）；河北省科学院高层次人才培养与资助项目（2018G05）；河北省科学院科技计划项目（18301，19301）

通讯作者: 程辉彩,研究员,huicaicheng@163.com E-mail: huicaicheng@163.com

作者简介: 方楠(1994-),男,河北省石家庄人,工程师,硕士,主要研究方向为固废处理及厌氧发酵.发表论文5篇.

引用本文:

方楠, 赵燕肖, 习彦花, 刘敬, 梁文华, 程辉彩, 张丽萍. 金属离子对青霉素菌渣厌氧发酵产气模型分析[J]. 中国环境科学, 2020, 40(7): 3020-3028. FANG Nan, ZHAO Yang-Xiao, XI Yang-Hua, WU Jian, LIANG Wen-Hua, CHENG Hui-Cai, ZHANG Li-Ping. Different mathematical models in the analysis of the effect of trace elements on the anaerobic digestion of penicillin fermentation residues. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(7): 3020-3028.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2020/V40/I7/3020

[1]	张蒙蒙,陈雄木,李领肖,等.废菌渣基/微中孔活性炭的制备与结构表征[J]. 化工进展, 2018,37(12):4773-4781. Zhang Meng-Meng, Chen Xiong-Mu, Li Ling-Xiao, et al. Preparation and characterization of micro/mesoporous activated carbon from denitrided residue of waste penicillin mycelium[J]. Chemical Industry and Engineering Progress. 2018,37(12):4773-4781.
[2]	王慧,习彦花,崔冠慧,等.头孢菌素菌渣中温厌氧资源化处理[J]. 中国沼气. 2016,34(1):19-24. Wang Hui, Xi Yan-Hua, Cui Guan-Hui, et al. The Resource Utilization of Cephalosporins Bacterial Residue by Anaerobic Digestion under Mesophilic Temperature[J]. China Biogas, 2016, 34(1):19-24.
[3]	陈立文,方森海,王明兹.抗生素发酵废菌渣的无害化及资源再利用研究进展[J]. 生物技术通报, 2015,31(5):13-19. Chen Li-Wen, Fang Sen-Hai, Wang Ming-Zi. Research progress on the harmless treatment and resource reuse of antibiotic bacteria residues[J]. Biotechnology Bulletin, 2015,31(5):13-19.
[4]	Fierro J, Martinez E J, Rosas J G, et al. Co-digestion of swine manure and crude glycerine:Increasing glycerine ratio results in preferential degradation of labile compounds[J]. Water, Air, & Soil Pollution. 2016,227(3).
[5]	Bayr S, Pakarinen O, Korppoo A, et al. Effect of additives on process stability of mesophilic anaerobic monodigestion of pig slaughterhouse waste[J]. Bioresource Technology. 2012,120:106-113.
[6]	Banks C J, Zhang Y, Jiang Y, et al. Trace element requirements for stable food waste digestion at elevated ammonia concentrations[J]. Bioresource Technology, 2012,104:127-135.
[7]	Khatri S, Wu S, Kizito S, et al. Synergistic effect of alkaline pretreatment and Fe dosing on batch anaerobic digestion of maize straw[J]. Applied Energy. 2015,158:55-64.
[8]	吴树彪,郎乾乾,张万钦,等.微量元素对餐厨垃圾厌氧发酵的影响实验[J]. 农业机械学报, 2013,44(11):128-132. Wu Shu-Biao, Lang Qian-Qian, Zhang Wan-Qin, et al. Effect of trace elements on anaerobic digestion of food waste[J]. Transactions of the Chinese Society for Agricultural Machinery, 2013, 44:128-132.
[9]	Amir S, Hafidi M, Merlina G, et al. Sequential extraction of heavy metals during composting of sewage sludge[J]. Chemosphere, 2005, 59(6):801-810.
[10]	赵燕肖,习彦华,饶硕,等.碱热预处理对青霉素菌渣厌氧发酵产沼气效率的影响[J]. 中国沼气, 2017,35(5):3-8. Zhao Yan-Xiao, Xi Yan-Hua, Rao Shuo, et al. Effct of thermo-alkaline pretreatment on anarobic fermentation of penicillin mycelium residues[J]. China Biogas, 2017,35(5):3-8.
[11]	李轶,李磊,张大雷,等.餐厨垃圾和牛粪混合厌氧发酵工艺优化[J]. 农业机械学报, 2012,43(z1):180-185. Li Yi, Li Lei, Zhang Da-Lei, et al. Process optimization of anaerobic fermentation with mixed material of food garbage and cow dung[J]. Transactions of the Chinese Society for Agricultural Machinery, 2012,43(z1):180-185.
[12]	张万钦,吴树彪,郎乾乾,等.微量元素对沼气厌氧发酵的影响[J]. 农业工程学报, 2013,(10):1-11. Zhang Wan-Qin, Wu Shu-Biao, Lang Qian-Qian, et al. Trace elements on influence of anaerobic fermentation in biogas projects[J]. Transactions of the Chinese Society of Agricultural Engineering, 2013, (10):1-11.
[13]	Ameer K, Bae S, Jo Y, et al. Optimization of microwave-assisted extraction of total extract, stevioside and rebaudioside-A from Stevia rebaudiana (Bertoni) leaves, using response surface methodology (RSM) and artificial neural network (ANN) modeling[J]. Food Chemistry, 2017,229:198-207.
[14]	Dantas T N C, Cabral T J O, DantasNeto A A, et al. Enrichmnent of patchoulol extracted from Patchouli (Pogostemon Cablin) oil by molecular distillation using Response Surface and Artificial Neural Network Models[J]. Journal of Industrial and Engineering Chemistry, 2020,81:219-227.
[15]	Mohammadi F, Samaei M R, Azhdarpoor A, et al. Modelling and optimizing pyrene removal from the soil by phytoremediation using response surface methodology, artificial neural networks, and genetic algorithm[J]. Chemosphere, 2019,237:124486.
[16]	唐铭,冼萍,徐英博,等.Fe2+、Co2+、Ni2+对渗滤液厌氧过程的影响[J]. 环境科学学报, 2014,34(10):2573-2579. Tang Ming, Xian Ping, Xu Ying-Bo, et al. Effects of Fe2+, Co2+ and Ni2+ on anaerobic process for landfill leachate treatment[J]. Acta Scientiae Circumstantiae, 2014,34:2573-2579.
[17]	Zhang W, Zhang L, Li A. Enhanced anaerobic digestion of food waste by trace metal elements supplementation and reduced metals dosage by green chelating agent[S, S]-EDDS via improving metals bioavailability[J]. Water Research, 2015,84:266-277.
[18]	卢菊仪,高郁,常滨.铁离子对内循环厌氧反应器运行效能的影响[J]. 黑龙江科技学院学报, 2007,17(2):123-125,140. Lu Ju-Yi, Gao Yu, Chang Bin. Iron effect on efficiency of internal circulation anaerobic reactor[J]. Journal of Heilongjiang Institute of Science and Technology, 2007,17(2):123-125,140.
[19]	杨光,张光明,张盼月,等.微量元素Fe、Ni对污泥厌氧消化优化调理[J]. 环境工程学报, 2017,11(9):4971-4977. Yang Guang, Zhang Guang-Ming, Zhang Pan-Yue, et al. Enhancement of sludge anaerobic digestion by adding trace element Fe and Ni[J]. Chinese Journal of Environmental Engineering, 2017, 11(9):4971-4977.
[20]	方楠,程辉彩,吴健,等.青霉素菌渣厌氧发酵沼液对红三叶种子萌发及幼苗生长的影响[J]. 环境污染与防治, 2019,41(7):762-766. Fang Nan, Cheng Hui-Cai, Wu Jian, et al. The effects of biogas slurry of penicillin fermentation residues on seed germination and growth of Trifoliumpratense[J]. Environmental Pollution and Control, 2019,41(7):762-766.
[21]	陈保冬,李晓林,朱永官.丛枝菌根真菌菌丝体吸附重金属的潜力及特征[J]. 菌物学报, 2005,24(2):283-291. Chen Bao-Dong, LI Xiao-Lin, Zhu Yong-Guan. Characters of metal adsorption by am fungal mycelium[J]. Mycosystema, 2005,24:283-291.
[22]	Moestedt J, Nordell E, ShakeriYekta S, et al. Effects of trace element addition on process stability during anaerobic co-digestion of OFMSW and slaughterhouse waste[J]. Waste Management, 2016,47:11-20.
[23]	Bartacek J, Fermoso F G, Baldó-Urrutia A M, et al. Cobalt toxicity in anaerobic granular sludge:influence of chemical speciation[J]. Journal of Industrial Microbiology & Biotechnology, 2008,35(11):1465-1474.
[24]	Han K, Levenspiel O. Extended monod kinetics for substrate, product, and cell inhibition[J]. Biotechnol Bioeng, 1988,32(4):430-447.
[25]	Zhang R, Elmashad H, Hartman K, et al. Characterization of food waste as feedstock for anaerobic digestion[J]. Bioresource Technology, 2007,98(4):929-935.
[26]	农丽薇,徐龙君,谢金连,等.微量Co和Ni对稻草厌氧消化的影响[J]. 环境科学研究, 2008,21(2):163-167. Nong Li-Wei, Xu Long-Jun, Xie Jin-Lian, et al. The Effect of Trace Co and Ni on the anaerobic digestion of straw[J]. Research of Environmental Sciences. 2008,21(2):163-167.
[27]	Ashley N V, Davies M, Hurst T J. The effect of increased nickel ion concentrations on microbial populations in the anaerobic digestion of sewage sludge[J]. Water Research, 1982,16(6):963-971.
[28]	Xu C, Wang J, Yang T, et al. Adsorption of uranium by amidoximated chitosan-grafted polyacrylonitrile, using response surface methodology[J]. Carbohydrate Polymers, 2015,121:79-85.
[29]	赵剑斐,张毅博,黄涛,等.基于响应曲面法的餐厨垃圾与剩余污泥混合厌氧发酵工艺优化[J]. 安全与环境学报, 2019,19(4):1316-1322. Zhao Jian-Fei, Zhang Yi-Bo, Huang Tao, et al. Anaerobic Co-digestion processing optimization for the food waste and sewage sludge based on response surface method[J]. Journal of Safety and Environment, 2019,19:1316-1322.
[30]	Pobeheim H, Munk B, Lindorfer H, et al. Impact of nickel and cobalt on biogas production and process stability during semi-continuous anaerobic fermentation of a model substrate for maize silage[J]. Water Research, 2011,45(2):781-787.
[31]	李小兰,周芸,陈志燕,等.针铁矿强化剩余污泥与烟草废弃物厌氧发酵的研究[J]. 中国环境科学, 2019,39(6):2475-2482. Zhou X L, Zhou Y, Chen Z Y, et al. Enhanced anaerobic fermentation of waste activated sludge and tobacco waste using Goethite[J]. China Environmental Science, 2019,45(6):2475-2482.
[32]	贾丽娟,俞芳,宁平,等.温度底物浓度和微量元素对牛粪厌氧发酵产沼气的影响[J]. 农业工程学报, 2014,30(22):260-266. Jia Li-Juan, Yu Fang, Ning Ping, et al. Influence of temperature, concentration of substrate and trace elements on anaerobic fermentation with dairy manure[J]. Transactions of the Chinese Society of Agricultural Engineering, 2014,30(22):260-266.
[33]	Ferry J G. Enzymology of one-carbon metabolism in methanogenic pathways[J]. FEMS Microbiology Reviews, 1999,23(1):13-38.
[34]	李亚新,董春娟.激活甲烷菌的微量元素及其补充量的确定[J]. 环境污染与防治, 2001,23(3):116-118. Li Ya-Xin, Dong Chun-Juan. Determining trace metals stimulating methanogens and their supplement dosage[J]. Environmental Pollution & Control. 2001,23(3):116-118.
[35]	Cao W, Wang M, Liu M, et al. The chemical and dynamic distribution characteristics of iron, cobalt and nickel in three different anaerobic digestates:Effect of pH and trace elements dosage[J]. Bioresource Technology, 2018,269:363-374.
[36]	Thanh P M, Ketheesan B, Yan Z, et al. Trace metal speciation and bioavailability in anaerobic digestion:A review[J]. Biotechnology Advances, 2016,34(2):122-136.
[37]	刘亚利,余紫薇,孟小凡,等.Fe2+投加方式对餐厨垃圾厌氧产甲烷的影响研究[J]. 应用化工, 2019,48(4):838-840. Liu Ya-Li, Yu Zi-Wei, Meng Xiao-Fan, et al. Effects of Fe2+ dosing methods on anaerobic digestion of kitchen waste[J]. Applied Chemical Industry. 2019,48(4):838-840.