Abstract:The objective of this study was to estimate the efficiency of SCFA production from primary sludge using bio- enzyme regulation including alkaline protease, neutral protease and α-amylase. The regulation mechanism was revealed by microbial community and SCFAs component analyses. The three kinds of bio-enzymes could enhance the hydrolysis and acid production during the fermentation of primary sludge. Compared with the blank group, the production and yield of SCFAs achieved 1508 mg COD/L and 0.174 g COD/g VSS on the fourth day of fermentation, respectively, increasing by 1129 mg COD/L and 0.13 g COD/g VSS. Microbial community structure analysis showed that the relative abundance of fermentation-relating bacteria such as Lentimicrobium, Proteiniphilum and Bacteroides were improved, and the growth of methanogenic archaea such as Methanosaeta and Methanosapirillum was inhibited when bio-enzymes were added into the fermentation system. At the same time, the production of acetic acid in the regulated fermentation process was also found to promote.
刘国华, 王健, 齐鲁, 王洪臣. 生物酶对初沉污泥厌氧发酵产短链脂肪酸的调控研究[J]. 中国环境科学, 2022, 42(5): 2195-2203.
LIU Guo-hua, WANG Jian, QI Lu, WANG Hong-chen. Study on regulating anaerobic fermentation for producing short-chain fatty acids from primary sludge in WWTPs by different bio-enzymes. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(5): 2195-2203.
黄 潇,董文艺,赵福祥,等.发酵周期对初沉污泥厌氧发酵产酸影响及微生物机制研究 [J]. 环境科技, 2019,32(2):37-43. Huang X, Dong W Y, Zhao F X, et al. Effect of fermentation cycle on acid production by anaerobic fermentation of primary sludge and microbial mechanism [J]. Environmental Science and Technology, 2019,32(2):37-43.
[2]
Banister S, Pretorius W A. Optimization of primary sludge acidogenic fermentation for biological nutrient removal [J]. Water S A, 1998.
[3]
Pitman A R, Lotter L H, Alexander W V, et al. Fermentation of raw sludge and elutriation of resultant fatty acids to promote excess biological phosphorus removal [J]. Water Science & Technology, 1992, 25(4):185-194.
[4]
罗 琨,杨 麒,李小明,等.外加酶强化剩余污泥水解的研究 [J]. 环境科学, 2010,31(3):763-767. Luo K, Yang Q, Li X M, et al. Study on enhanced hydrolysis of excess sludge with additional enzyme [J]. Environmental Science, 2010,31(3): 763-767.
[5]
徐 友,陈思思,董 滨,等.酶处理强化污泥厌氧消化效能及脱水性能的研究进展 [J]. 工业水处理, 2018,38(3):6-11. Xu Y, Chen S S, Dong B, et al. Research progress on sludge anaerobic digestion efficiency and dewatering performance enhanced by enzyme treatment [J]. Industrial Water Treatment, 2018,38(3):6-11.
[6]
Diak J, Oermeci B, Kennedy K J. Effect of enzymes on anaerobic digestion of primary sludge and septic tank performance [J]. Bioprocess & Biosystems Engineering, 2012,35(9):1577.
[7]
Yu S, Zhang G, Li J, et al. Effect of endogenous hydrolytic enzymes pretreatment on the anaerobic digestion of sludge [J]. Bioresource Technology, 2013,146:758-761.
[8]
耿娜瑶.酶促厌氧污泥水解研究 [D]. 大连:大连海事大学, 2017. Geng N Y. Study on enzymatic anaerobic sludge hydrolysis [D]. Dalian: Dalian Maritime University, 2017.
[9]
国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法 [M]. 4版.北京:中国环境科学出版社, 2002. Editorial board of "water and wastewater monitoring and analysis methods" of the State Environmental Protection Administration. Monitoring and analysis methods of water and wastewater [M]. Fourth Edition. Beijing: China Environmental Science Press, 2002.
[10]
Lowry O H, Rosebrough N J, Farr A L, et al. Protein measurement with the Folin phenol reagent [J]. J. Biol. Chem., 1951,193:265-275.
[11]
Grady C. Biological wastewater treatment [M]. New York: Marcel Dekker, 1980.
[12]
He L, Jin W, Liu X, et al. Acidogenic fermentation of proteinaceous sewage sludge: Effect of pH [J]. Water Research, 2012,46(3):799-807.
[13]
Chen H B, Chang S. Impact of temperatures on microbial community structures of sewage sludge biological hydrolysis [J]. Bioresour. Technol., 2017,245(Pt.A).502-510.
[14]
辛晓东.酶溶剩余污泥发酵产酸效能与机制研究 [D]. 哈尔滨:哈尔滨工业大学, 2018. Xin X D. Study on efficiency and mechanism of acid production by enzymatic digestion of excess sludge [D]. Harbin: Harbin Institute of technology, 2018.
[15]
Novak J T, Sadler M E, Murthy S N. Mechanisms of floc destruction during anaerobic and aerobic digestion and the effect on conditioning and dewatering of biosolids [J]. Water Research, 2003,37(13):3136- 3144.
[16]
Li H, Si D D, Liu C, et al. Performance of direct anaerobic digestion of dewatered sludge in long-term operation [J]. Bioresource Technology, 2017,250:355.
[17]
宋小莉,施正华,李秀芬,等.基于蛋白质回收的剩余污泥酶解技术研究 [J]. 食品与生物技术学报, 2019,38(4):90-96. Song X L, Shi Z H, Li X F, et al. Study on enzymatic hydrolysis technology of excess sludge based on protein recovery [J]. Journal of Food and Biotechnology, 2019,38(4):90-96.
[18]
Higuchi Y, Ohashi A, Imachi H, et al. Hydrolytic activity of alpha- amylase in anaerobic digested sludge [J]. Water Science & Technology, 2005,52(1/2):259-266.
[19]
苑宏英.基于酸碱调节的剩余污泥水解酸化及其机理研究 [D]. 上海:同济大学, 2006. Yuan H Y. Study on hydrolysis and acidification of excess sludge based on acid-base regulation and its mechanism [D]. Shanghai: Tongji University, 2006.
[20]
Xiong H L, Chen J L, Wang H, et al. Influences of volatile solid concentration, temperature and solid retention time for the hydrolysis of waste activated sludge to recover volatile fatty acids [J]. Bioresource Technology, 2012,119:285-92.
[21]
罗 琨.外加水解酶强化剩余污泥水解和酸化的研究 [J]. 长沙:湖南大学, 2013. Luo K. Study on enhanced hydrolysis and acidification of excess sludge with additional hydrolase [J]. Changsha: Hunan University, 2013.
[22]
苏高强.剩余污泥碱性发酵产酸性能与优化 [D]. 北京:北京工业大学, 2013. Su G Q. Acid production performance and optimization of excess sludge alkaline fermentation [D]. Beijing: Beijing University of Technology, 2013.
[23]
Rashed A A, Akunna J C, El-halwany M M, et al. Improvement in the efficiency of hydrolysis of anaerobic digestion in sewage sludge by the use of enzymes [J]. Desalination and Water treatment, 2010,21(1-3): 280-285.
[24]
Noyola A, Tinajero A. Effect of biological additives and micronutrients on the anaerobic digestion of physicochemical sludge [J]. Water Science & Technology, 2005,52(1/2):275-281.
[25]
Zheng X, Su Y L, Li X, et al. Pyrosequencing reveals the key microorganisms involved in sludge alkaline fermentation for efficient short-chain fatty acids production [J]. Environmental Science & Technology, 2013,47(9):4262-4268.
[26]
Sun L W, Toyonaga M, Ohashi A, et al. Lentimicrobium saccharophilum gen. nov., sp. nov., a strictly anaerobic bacterium representing a new family in the phylum Bacteroidetes, and proposal of Lentimicrobiaceae fam. nov. [J]. Int. J. Syst. Evol. Microbiol., 2016, 66(7):2635-2642.
[27]
Flint H J, Duncan S H. Bacteroides and Prevotella [M]//Encyclopedia of food microbiology. 2nd Edition. 2014:203-208.
[28]
Jabari L, Gannoun H, Cayol J L, et al. Macellibacteroides fermentans gen. nov., sp. nov., a member of the family Porphyromonadaceae isolated from an upflow anaerobic filter treating abattoir wastewaters [J]. International Journal of Systematic and Evolutionary Microbiology, 2012,62(10): 2522-2527.
[29]
Chen S Y, Dong X Z, et al. Proteiniphilum acetatigenes gen. nov., sp. nov., from a UASB reactor treating brewery wastewater [J]. Int. J. Syst. Evol. Microbiol., 2005,55.0(6):2257-2261.
[30]
Hania W B, Godbane R, Postec A, et al. Defluviitoga tunisiensis gen. nov., sp. nov., a thermophilic bacterium isolated from a mesothermic and anaerobic whey digester [J]. International Journal of Systematic and Evolutionary Microbionary Microbiology, 2011,62(6):1377.
[31]
Lawson P A. Fastidiosipila [M]. John Wiley & Sons, Ltd, 2015.