Effect of C/N/S on nitrate reduction process in activated sludge system
ZHANG Peng-cheng1, LI Xiao-ling1,2, WANG Xiao-ting1, ZHANG Yu-hao3, ZHANG Jia-ying1, LIU Xin-yi1, ZHANG Wen-bo1
1. School of Civil Engineering, Chang'an University, Xi'an 710061, China; 2. Key Laboratory of Water Supply & Sewage Engineering, Ministry of Housing and Urban-Rural Development, School of Civil Engineering, Chang'an University, Xi'an 710054, China; 3. Xi'an Zhongjiao Environmental Engineering Co., Ltd., Xi'an 710054, China
Abstract：The effect of different electron donors (organics or S2-) on denitrification and nitrate nitrogen reduction to ammonium (DNRA) was explored by adjusting inlet C/N and S/N ratios in an activated sequencing batch reactor (ASBR). The results showed that higher C/N ratios improved denitrification process, while higher S/N ratios were more beneficial to DNRA process. NH4+-N, the characteristic product of DNRA, greatly yielded when C/N/S were 2:2:3 and 2:2:4, with the highest effluent NH4+-N concentration of 10.65mg/L at C/N/S of 2:2:4. It indicated that when the electron acceptor was limited, the excess electron donor could increase the activity of DNRA and promote the conversion of denitrification to the DNRA process. 16SrRNA microbiological analysis clarified that Proteobacteria, Anaerolineae, Bacteroidia as well as Actinobacteria were closely related with nitrate reduction process, and Actinobacteria was corelated with the DNRA. Overall, this study provided alternative strategies for the simultaneous removal of carbon, nitrogen, and sulfur in the sewage treatment process.
姜巍.生物反硝化去除饮用水中硝酸盐氮的研究[D]. 北京:中国科学院生态环境研究中心, 2001. Jiang W. Study on the removal of nitrate nitrogen in drinking water by biological denitrification[D]. Beijing:Research Center for Ecological Environment, Chinese Academy of Sciences, 2001.
Li Z X, Peng Y Z, Gao H J. Enhanced long-term advanced denitrogenation from nitrate wastewater by anammox consortia:Dissimilatory nitrate reduction to ammonium (DNRA) coupling with anammox in an upflow biofilter reactor equipped with EDTA-2Na/Fe(Ⅱ) ratio and pH control.[J] Bioresource Technology, 2020, 305,123083.
薛惊雅,姜星宇,姚晓龙,等.傀儡湖沉积物-水界面硝酸盐异养还原过程研究[J]. 中国环境科学. 2018,38(6):2289-2296. Xue J Y, Jiang X Y, Yao X L, et al. Study on the process of nitrate heterotrophic reduction at sediment-water interface in Puppet Lake[J]. Environmental Science in China, 2018,38(6):2289-2296.
Giblin A E, Tobias C R, Song B, et al. The importance of dissimilatory nitrate reduction to ammonium (DNRA) in the nitrogen cycle of coastal ecosystems[J]. Oceanography, 2013,26(3):124-131.
张新艳,彭党聪,万琼,等.活性污泥中硝酸盐异化还原成铵(DNRA)过程及其影响因素[J]. 环境保护前沿, 2018,8(2):95-105. Zhang X Y, Peng D C, Wan Q, et al. Dissimilation and reduction of nitrate in activated sludge to ammonium (DNRA) and its influencing factors[J]. Frontier of Environmental Protection, 2018,8(2):95-105.
张宇浩,李晓玲,陈莹,等.C/S对生物氮转移途径的影响[J]. 环境工程, 2019,37(12):6-11. Zhang Y H, Li X L, Chen Y, et al. The influence of C/S on biological nitrogen transfer pathway[J]. Environmental Engineering, 2019, 37(12):6-11.
何秋来.厌氧/好氧/缺氧同步硝化反硝化除磷颗粒污泥系统构建及强化策略研究[D]. 武汉:武汉大学, 2019. He Q L. Anaerobic/aerobic/anoxic simultaneous nitrification and denitrification of phosphorus removal granular sludge system construction and strengthening strategy research[D]. Wuhan:Wuhan University, 2019.
国家环境保护总局.水和废水监测分析方法.第4版[M]. 北京:中国环境科学出版社.2002. National Environmental Protection Administration. Water and wastewater monitoring and analysis methods[M]. Beijing:China Environmental Science Press, 2002.
詹文毅,江国庆,姜国民,等.紫外分光光度法直接测定常量硫酸根[J]. 理化检验(化学分册), 2016,52(2):159-162. Zhan W Y, Jiang G Q, Jiang G M, et al. Direct determination of macro sulfate by ultraviolet spectrophotometry[J]. Physical and Chemical Inspection (Chemical Section), 2016,52(2):159-162.
Manconi I, Carucci A, Lens P. Combined removal of sulfur compounds and nitrate by autotrophic denitrification in bioaugmented activated sludge system[J]. Biotechnology & Bioengineering, 2007,98(3):551-560.
Chutivisut P, Isobe K, Powtongsook S, et al. Distinct microbial community performing dissimilatory nitrate reduction to ammonium (DNRA) in a high C/NO3- reactor[J]. Microbes and Environments, 2018,33(3):264-271.
刘双,赵剑强,王莎,等.硫自养与异养混合亚硝酸盐反硝化过程铵生成机制[J]. 环境工程学报, 2019,13(6):1366-1373. Liu S, Zhao J Q, Wang S, et al. The mechanism of ammonium formation during sulfur autotrophic and heterotrophic mixed nitrite denitrification[J]. Journal of Environmental Engineering, 2019,13(6):1366-1373.
Ye J, Joseph S D, Ji M, et al. Chemolithotrophic processes in the bacterial communities on the surface of mineral-enriched biochars[J]. The ISME Journal Emultidisciplinary Journal of Microbial Ecology, 2017,11(5):1087-1101.
罗剑飞.硫氧化菌群落结构分析及其特性研究[D]. 广州:华南理工大学, 2011. Luo J F. Study on the community structure and characteristics of sulfur-oxidizing bacteria[D]. Guangzhou:South China University of Technology, 2011.
Nakagawa T, Tsuchiya Y, Ueda S, et al. Eelgrass sediment microbiome as a nitrous oxide sink in Brackish Lake Akkeshi, Japan[J]. Microbes and Environments, 2019,34(1):13-22.
Tian H, Liu J, Feng T, et al. Assessing the performance and microbial structure of biofilms adhering on aerated membranes for domestic saline sewage treatment[J]. RSC Advances, 2017,7(44):27198-27205.
赵明明,赵鑫盟,希尼尼根,等.农田土壤nirK和nirS型反硝化微生物的研究进展[J]. 微生物前沿, 2018,7(2):65-72. Zhao M M, Zhao X M, Xini N G, et al. Research progress of nirK and nirS denitrifying microorganisms in farmland soil[J]. Advances in Microbiology, 2018,7(2):65-72.