Abstract：In this study, biochar (BC) was prepared from rice straw at different pyrolysis temperatures (300, 400, and 500℃), and was used for dissolved fractions (DBC) extraction. In this study, the effects of BC and DBC on the reduction of ferrihydrite by Geobacter sulphurreducens PCA were investigated by combining microbial reduction experiments and various characterization methods including Fourier transition infrared spectroscopy (FTIR), X-ray diffraction crystal diffraction (XRD), and Electron paramagnetic resonance (EPR). The results showed that the highest reduction rate of ferrihydrite was achieved after the addition of BC prepared at 400℃ (BC-400), which increased the rate of microbial dissimilatory iron reduction by 12 times. Containing the most quinone and carboxyl groups, BC-400 could function as an electron shuttle to promote electron transfer. BC could not serve as an electron donor to provide electrons directly to PCA or ferrihydrite. DBC increased the degree of long-term microbial reduction extent and initial reduction rate by more than 10 times and 2 times, respectively. DBC extracted from BC prepared at 500℃ served as an both electron shuttle and electron donor to promote the microbial reduction of ferrihydrite, but it cannot directly chemically reduce ferrihydrite.
夏金霞, 孙金涛, 于蕊, 王一初, 安伟奇, 金洁, 曹丹丹. 生物炭及其水溶组分促进水铁矿微生物还原[J]. 中国环境科学, 2023, 43(10): 5422-5432.
XIA Jin-xia, SUN Jin-tao, YU Rui, WANG Yi-chu, AN Wei-qi, JIN Jie, CAO Dan-dan. Effects of biochar and its dissolved fractions on microbial reduction of ferrihydrite. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(10): 5422-5432.
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