改性稻壳生物炭对水中反硝化过程和N<sub>2</sub>O排放的影响

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摘要为探究H₂O₂改性和NaBH₄改性稻壳生物炭(BC-H₂O₂和BC-NaBH₄)对反硝化过程和N₂O排放的影响及机理, 在制备并测定BC-H₂O₂和BC-NaBH₄理化性质及其表面含氧官能团含量基础上, 将未改性生物炭(BC)、BC-H₂O₂和BC-NaBH₄以1%(w/V)的比例分别加入含有厌氧反硝化细菌(DB)的培养体系, 开展DB去除模拟废水中低浓度硝酸盐(约10mg/L, 以N计)的室内培养实验. 结果表明, H₂O₂改性增加了生物炭表面的羧基含量, 而NaBH₄改性增加了生物炭表面的内酯基和酚羟基含量. 另外, 傅立叶变换红外光谱分析表明, 与BC相比, BC-H₂O₂的C=O含量明显增加. DB+BC-H₂O₂和DB+BC-NaBH₄处理的反硝化速率峰值较DB+BC处理提前12h出现, 且分别高17.50%和6.32%. 与DB+BC处理相比, DB+BC-NaBH₄处理的N₂O累积排放量增加10.43%, 但差异不显著; DB+BC-H₂O₂处理的N₂O累积排放量显著增加165.54%, N₂O/(N₂O+N₂)比值显著增加170.00%, 但N₂O+N₂累积排放量之间无显著差异(P<0.05), 表明BC-H₂O₂抑制反硝化过程中N₂O向N₂还原, 进而促进N₂O排放, 这可能与添加BC-H₂O₂使培养体系的pH值、碳生物有效性降低以及C=O含量增加有关.

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	王朝旭
	刘勇超
	常智淋
	陈启斌
	王永
	李作臣
	闫铭
	魏阳

关键词 ：改性稻壳生物炭, 硝酸盐, 反硝化作用, N₂O排放

Abstract：The study focused on exploring the effects of H₂O₂-and NaBH₄-modified rice husk-derived biochars (BC-H₂O₂ and BC-NaBH₄) on denitrification and N₂O emission and the potential mechanisms. Based on BC-H₂O₂ and BC-NaBH₄ preparation and their physicochemical property and surface oxygen-containing functional group content determination, the pristine rice husk-derived biochar (BC), BC-H₂O₂, and BC-NaBH₄ were added into the incubation system containing the screened anaerobic denitrifier (DB) at the ratio of 1% (w/V), respectively. The microcosm incubation experiment was carried out to explore the removal efficiency of nitrate nitrogen (about 10mg/L) from simulated wastewater by DB. Results showed that H₂O₂-modification increased the carboxyl content on the surface of biochar, while NaBH₄-modification increased the contents of lactone and phenolic hydroxyl on the surface of biochar. In addition, the Fourier transform infrared spectra analysis showed that C=O content of BC-H₂O₂ was significantly higher than that of BC. The denitrification rate peaks of DB+BC-H₂O₂ and DB+BC-NaBH₄ appeared 12h earlier and their values were 17.50% and 6.32% higher than that of DB+BC, respectively. Compared with DB+BC, the cumulative N₂O emission of DB+BC-NaBH₄ increased by 10.43%, but the difference was not significant. However, the cumulative N₂O emission of DB+BC-H₂O₂ increased by 165.54% and the N₂O/(N₂O+N₂) ratio increased by 170.00% significantly, while there was no significant difference in the cumulative N₂O+N₂ emission between DB+BC-H₂O₂ and DB+BC (P<0.05). The result indicated that BC-H₂O₂ inhibited N₂O reduction to N₂ and promoted N₂O emission, which might be related to the decrease of pH and carbon bioavailability and the increase of C=O content induced by BC-H₂O₂ amendment.

Key words： modified rice husk-derived biochar nitrate denitrification N₂O emission

收稿日期: 2022-10-30

PACS:

X52

基金资助:山西省自然科学基金资助项目(201901D111066)

通讯作者: 王朝旭,副教授,cxwang127@126.com E-mail: cxwang127@126.com

作者简介: 王朝旭(1981-),男,河南郑州人,副教授,博士,主要从事生物质炭的水土环境效应研究.发表论文20篇.cxwang127@126.com

引用本文:

王朝旭, 刘勇超, 常智淋, 陈启斌, 王永, 李作臣, 闫铭, 魏阳. 改性稻壳生物炭对水中反硝化过程和N₂O排放的影响[J]. 中国环境科学, 2023, 43(6): 2908-2916. WANG Chao-xu, LIU Yong-chao, CHANG Zhi-lin, CHEN Qi-bin, WANG Yong, LI Zuo-chen, YAN Ming, WEI Yang. Effects of modified rice husk-derived biochar on denitrification and N₂O emission from water. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(6): 2908-2916.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I6/2908

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