顶/底部变频交替进水的厌氧氨氧化系统特性

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摘要 The granulation process of anaerobic ammonia oxidation sludge is slow and easily affected by hydraulic shear force and fluctuation in substrate concentration. At the same time, the mass transfer performance of granular sludge deteriorates with the increase of particle size, which leads to the limitation of nitrogen removal performance of the system. In this paper, a top/bottom variable frequency alternating influent operation mode was proposed, which extends the inlet duration and introduces recirculation to provide sufficient hydraulic shear forces. By varying the inlet positions and frequencies, this method effectively reduces the impact load of substrate concentration on Anammox bacteria and enhances the system's nitrogen removal performance. The changes in sludge concentration, particle performance, EPS(Extracellular Polymeric Substances) secretion and nitrogen removal stability during operation were investigated. The results show that under this influent mode, the removal rate of total nitrogen reached 84.80 %, with an average particle size of 783.67μm, and EPS content of 169.13mg/gVSS. After increasing the influent load, the maximum concentration of free ammonia was 9.70mg/L, which has no inhibitory effect on the system. Microscopic observations revealed darker granule colors and higher heme c content, indicating better AnAOB activity. The high-throughput sequencing results showed that the top/bottom variable frequency alternating influent operation mode can greatly increase the abundance of anaerobic ammonia oxidizing bacteria Candidatus_Brocadia in the system, reaching a peak of 43.59% when the alternating frequency was 3times. The influent mode accelerates the mixing of sludge and substrate, shortens the granulation process, improves sludge activity and resilience to shock loads, and realizes the stable operation of the anaerobic ammonia oxidation system.

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关键词 ：顶底部变频交替进水, 游离氨, 回流系统, 饥饿时长

Abstract：The granulation process of anaerobic ammonia oxidation sludge is slow and easily affected by hydraulic shear force and fluctuation in substrate concentration. At the same time, the mass transfer performance of granular sludge deteriorates with the increase of particle size, which leads to the limitation of nitrogen removal performance of the system. In this paper, a top/bottom variable frequency alternating influent operation mode was proposed, which extends the inlet duration and introduces recirculation to provide sufficient hydraulic shear forces. By varying the inlet positions and frequencies, this method effectively reduces the impact load of substrate concentration on Anammox bacteria and enhances the system's nitrogen removal performance. The changes in sludge concentration, particle performance, EPS(Extracellular Polymeric Substances) secretion and nitrogen removal stability during operation were investigated. The results show that under this influent mode, the removal rate of total nitrogen reached 84.80 %, with an average particle size of 783.67μm, and EPS content of 169.13mg/gVSS. After increasing the influent load, the maximum concentration of free ammonia was 9.70mg/L, which has no inhibitory effect on the system. Microscopic observations revealed darker granule colors and higher heme c content, indicating better AnAOB activity. The high-throughput sequencing results showed that the top/bottom variable frequency alternating influent operation mode can greatly increase the abundance of anaerobic ammonia oxidizing bacteria Candidatus_Brocadia in the system, reaching a peak of 43.59% when the alternating frequency was 3times. The influent mode accelerates the mixing of sludge and substrate, shortens the granulation process, improves sludge activity and resilience to shock loads, and realizes the stable operation of the anaerobic ammonia oxidation system.

Key words： alternating influent at variable frequencies from top and bottom free ammonia reflux system starve duration

收稿日期: 2024-03-15

PACS:

X703.1

基金资助:北京高校卓越青年科学家计划项目(BJJWZYJH012-01910005019)

通讯作者: 李冬,教授,lidong2006@bjut.edu.cn E-mail: lidong2006@bjut.edu.cn

作者简介: 李冬(1976-),女,辽宁丹东人,教授,博士,主要研究方向为水环境恢复理论及关键技术.发表论文200余篇.lidong2006@bjut.edu.cn.

引用本文:

李冬, 陶博, 张杰. 顶/底部变频交替进水的厌氧氨氧化系统特性[J]. 中国环境科学, 2024, 44(11): 6057-6067. LI Dong, TAO Bo, ZHANG Jie. Characteristics of anaerobic ammonium oxidation (anammox) system featuring alternating influent at variable frequencies from top and bottom. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(11): 6057-6067.

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