表观气速对除磷污泥颗粒化性能及代谢特征的影响

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摘要以60%乙酸钠和40%可溶性淀粉复合基质为碳源，考察表观气速对生物除磷污泥颗粒化性能及代谢特征的影响.结果表明，与小分子碳源不同，复杂碳源条件下生物除磷污泥的颗粒化由剪切力和碳源的胞内储存共同决定，污泥颗粒化进程与表观气速的大小并不完全一致.R1表观气速最大（0.45cm/s），110d可实现颗粒化；R3表观气速最小（0.15cm/s），190d实现颗粒化，进程慢于R1，但快于R2（表观气速0.30cm/s）.降低表观气速污泥的丝状菌含量依次增多，紧密型胞外聚合物（TB-EPS）的疏水性依次降低；但系统更易实现厌氧饱食和好氧饥饿，聚羟基烷酸酯（PHAs）厌氧合成量依次升高，R1、R2、R3分别为2.28、8.16、23.96mg/gVSS.16S rRNA基因高通量测序表明，随着表观气速的降低，具有水解发酵功能的绿弯菌门（Chloroflexi）的丰度依次升高，分别为7.86%、8.77%和32.74%.在属水平，传统聚糖菌Candidatus_Competibacter是污泥颗粒化的核心菌群，R1较高的表观气速有利于该菌的维持和优势增殖，污泥颗粒化进程最快.R3表观气速最低，水解发酵功能菌群Kouleothrix（1851型丝状菌，属于绿弯菌门）大量生长（丰度10.44%），通过提供丝状骨架和小分子碳源促进Candidatus_Competibacter的优势增殖（丰度25.73%），加快了污泥的颗粒化.

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	陈希
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	蔡虎林
	曹银焕
	杨文浩
	穆瑞花

关键词 ：复杂碳源, 颗粒污泥, 表观气速, 胞内储存, 菌群结构, 影响

Abstract：In this study, the effect of superficial gas velocity on the granulation and metabolic characteristics of biological phosphorus removal sludge was investigated using a mixture of 60% sodium acetate and 40% soluble starch as the carbon source. The results showed that, unlike under the condition of small-molecule carbon source, the granulation of biological phosphorus removal sludge under complex carbon source condition was determined by both shear force and intracellular storage of carbon source, and the granulation process of the sludge was not completely consistent with the magnitude of the superficial gas velocity. R1 with the largest superficial gas velocity (0.45cm/s) achieved granulation on day 110, and R3 with the smallest superficial gas velocity (0.15cm/s) achieved granulation on day 190, which was slower than that in R1 but faster than that in R2 (0.30cm/s). Decreasing the superficial gas velocity successively increased the content of filamentous bacteria and decreased the hydrophobicity of the tightly bound extracellular polymeric substance (TB-EPS); but the system was easier to meet the demand of anaerobic feast and aerobic famine, and the content of polyhydroxyalkanoates (PHAs) from anaerobic synthesis increased, and the corresponding content in R1, R2, R3 was 2.28, 8.16 and 23.96mg/gVSS, respectively. High-throughput sequencing analysis of 16S rRNA genes showed that as the superficial gas velocity decreased, the abundances of Chloroflexi with hydrolysis and fermentation function successively increased, which were 7.86%, 8.77% and 32.74%, respectively. At the genus level, Candidatus_Competibacter, a typical glycogen accumulating organism, was the key group for sludge granulation. The high superficial gas velocity in R1was beneficial to the maintenance and proliferation of this group, and the granulation process in R1 was the fastest. The superficial gas velocity in R3 was the lowest, and Kouleothrix (filamentous bacterium type 1851, belonging to the Chloroflexi) with the function of hydrolysis and fermentation thrived (10.44% in abundance), which promoted the proliferation of Candidatus_Competibacter (25.73% in abundance) by providing filamentous skeleton and small-molecule carbon source, thus accelerating the granulation of the sludge.

Key words： complex carbon source granular sludge superficial gas velocity intracellular storage bacterial community structure effect

收稿日期: 2023-07-07

PACS:

X703.5

基金资助:陕西省自然科学基金（2021JQ-690）；陕西省重点研发计划项目（2023YBSF-531）；陕西省膜分离重点实验室开放基金（2022MFL01）

通讯作者: 陈希(1985-),讲师,Xi_Chen2011@126.com E-mail: Xi_Chen2011@126.com

作者简介: 陈希(1985-),女,陕西西安人,讲师,博士,主要从事好氧颗粒污泥技术研究、污水处理与资源化.发表论文15篇.Xi_Chen2011@126.com.

引用本文:

陈希, 胡彬, 张瑞峰, 宫延哲, 蔡虎林, 曹银焕, 杨文浩, 穆瑞花. 表观气速对除磷污泥颗粒化性能及代谢特征的影响[J]. 中国环境科学, 2024, 44(2): 686-698. CHEN Xi, HU Bin, ZHANG Rui-feng, GONG Yan-zhe, CAI Hu-lin, CAO Yin-huan, YANG Wen-hao, MU Rui-hua. Effect of superficial gas velocity on the granulation and metabolic characteristics of phosphorus removal sludge. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(2): 686-698.

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