Formation process and microscopic characteristics of autotrophic nitrifying granular sludge
LIU Chong1, LI Yu-guang1, CHEN Ning2, ZHANG Xin2, SHEN Fei3, WANG Shuo2,4, LI Ji2,4
1. 101 Institute of the Ministry of Civil Affairs, Beijing 100070, China; 2. Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China; 3. Laboratory of Instrumental Analysis, Jiangsu Wuxi Environmental Monitoring Center, Wuxi 214121, China; 4. Jiangsu College of Water Treatment Technology and Material Collaborative Innovation Center, Suzhou 215009, China
Abstract：In this study, mature aerobic granular sludge and flocculation activated sludge were both used as inoculation sludge in sequencing batch reactor (SBR) to rapidly culture ANGS. The experimental results showed that the addition of mature aerobic granular sludge is conducive to the rapid formation of ANGS and quick start-up of SBR. Aerobic granular sludge can provide the attachment carrier for nitrifying bacteria, and rapidly cultivate ANGS with good ammonia nitrogen removal performance. Extracellular polymeric substances (EPS) plays an important role in the formation process of ANGS, and tightly bound-EPS (TB-EPS) had the highest protein and polysaccharide contents, they reached 192.197 and 59.380mg/g vss, indicating that the microbial communication was intensive TB-EPS layer, and TB-EPS is beneficial to the formation of ANGS. Hydrophobic tryptophan and tyrosine up to 70% can provide more hydrophobic adsorption sites for soluble microbial products-EPS (SMP-EPS) and loosely bound-EPS (LB-EPS), thereby enhancing the adhesion of the sludge particles and promoting the formation of ANGS. In addition, the increased proportion of humic acid and soluble microbial metabolites in TB-EPS are beneficial to enhance the flocculation ability of ANGS. Glutamic acid, as the crossing point between carbon and nitrogen metabolism, accounts for 11.18% to 13.64%, is the basic amino acid of nitrogen metabolism, and the increase of proline content is helpful to maintain the stability of biofilm structure. The favorable aggregation structures including a-helix, b-angle and reverse b-folding of protein secondary structures in the amide Ⅰ region were much higher than the unfavorable aggregation structures, indicating that the stability of ANGS was enhanced during the granulation process.
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