硝化颗粒污泥胞外聚合物及信号分子功能分析

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摘要在SBR中以活性污泥为接种污泥培养自养硝化颗粒污泥.结果表明自养硝化颗粒污泥启动时间长,污泥颗粒化进程控制难度较大,但是仍能形成沉降性较好的自养硝化颗粒污泥,氨氮去除率能够达到90%以上.TB-EPS蛋白质和多糖含量占比最高,在第70d时TB-PN为90.69mg/gVSS,TB-PS为53.69mg/gVSS,TB-EPS作为污泥颗粒结构的最核心,在颗粒化过程中发挥重要作用.疏水性的色氨酸和酪氨酸有利于SMP层和LB-EPS增强污泥间的粘附性,从而促进自养硝化颗粒污泥的形成. 8种疏水性氨基酸的相对占比由初始的34%上升到最高66%,也表明疏水性氨基酸在絮状污泥颗粒化的关键时期发挥重要作用.聚合链相对占比的升高可以增强污泥间的结合力,β-折叠能提供较多的疏水位点从而促进硝化污泥的聚集,有利于自养硝化颗粒污泥的形成.C4-HSL浓度达到112.00ng/L,其可与LuxR蛋白结合可促进蛋白酶活性和EPS的产生.C6-HSL和C8-HSL对硝化菌群生物膜的形成具有调节作用, C6-HSL在第30d时达到30 μg/L,其含量最高表明其在自养硝化颗粒污泥的信号传递中占有重要地位.3OC6-HSL和蛋白质类物质呈正相关, 其与SMP-PN和LB-PN的相关性系数为0.77和0.71,可能通过指导蛋白质类物质的合成以增强污泥的粘附性从而有利于污泥微粒的聚集.

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关键词 ：自养硝化颗粒污泥, 胞外聚合物, EPS荧光光谱, 蛋白质二级结构, EPS氨基酸, AHLs

Abstract：Autotrophic nitrifying granular sludge (ANGS) was cultivated using activated sludge as the inoculated sludge in SBR. The cultivation of autotrophic nitrifying granular sludge was relatively long and the control of granulation process was difficult, however, ANGS with good sedimentation property can be obtained, and ammonia nitrogen removal was higher than 90%. TB-EPS had the highest proportion of protein and polysaccharide, and the respective content of TB-PN and TB-PS were 90.69mg/gVSS and 53.69mg/gVSS on the 70th day. As the core of sludge particle structure, TB-EPS played an important role in the granulation process. Hydrophobic tryptophan and tyrosine are beneficial to the SMP layer and LB-EPS to enhance the adhesion between the sludge, thereby promoting the formation of autotrophic nitrifying granular sludge. The relative proportion of 8hydrophobic amino acids increased from the initial 34% to the highest 66%, indicating that hydrophobic amino acids play an important role in the critical period of granulation process. Polymerase chain can enhance the binding force between sludge particles, and β- folding structure was able to provide more hydrophobic sites to promote the accumulation of the sludge particles, which was favorable to the formation of ANGS. The concentration of C4-HSL reached 112.00ng/L, and it could combine with LuxR protein to promote protease activity and EPS production. C6-HSL and C8-HSL could regulate the biofilm formation of nitrification bacteria. C6-HSL was up to 30μg/L on the 30th day, and its content was the highest, indicating that it plays an important role in the signal transmission of autotrophic nitrification granular sludge. 3OC6-HSL was positively correlated with protein substances, and its correlation coefficients with SMP-PN and LB-PN were 0.77 and 0.71, which may enhance the adhesion of sludge by guiding the synthesis of protein substances, thus facilitate the aggregation of sludge particles.

Key words： autotrophic nitrifying granular sludge extracellular polymeric substances (EPS) EPS fluorescence spectrum protein secondary structure EPS amino acids AHLs

收稿日期: 2023-02-10

PACS:	X703
	X172

基金资助:中央级公益性科研院所基本科研业务费专项(10211822011,0000009024)；中央级事业单位科研机构改善科研条件项目(10211822011, 0000009004)；江苏省政策引导类计划(国际科技合作/港澳台科技合作)专项资金资助项目(BZ2021030)；无锡市科技创新创业资金资助项目(M20211003)

通讯作者: 王硕,副教授,shuowang@jiangnan.edu.cn E-mail: shuowang@jiangnan.edu.cn

作者简介: 陈宁(1998-),女,山东临沂人,硕士研究生,主要从事污水处理技术和好氧颗粒污泥研究.发表论文1篇.chenning202103@163.com

引用本文:

陈宁, 刘崇, 李玉光, 张欣, 沈斐, 王硕, 李激. 硝化颗粒污泥胞外聚合物及信号分子功能分析[J]. 中国环境科学, 2023, 43(9): 4666-4675. CHEN Ning, LIU Chong, LI Yu-guang, ZHANG Xin, SHEN Fei, WANG Shuo, LI Ji. Characteristics of extracellular polymer substances and signal molecule function in autotrophic nitrifying granular sludge. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(9): 4666-4675.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I9/4666

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