Antioxidants alleviated sulfur dioxide-induced mitochondrial damage in rat hearts
XIA Jin1, QIN Guo-hua1,2, SANG Nan1
1. College of Environmental Science and Resources, Shanxi University, Taiyuan 030006, China;
2. Guangzhou Key Laboratory of Environmental Exposure and Health, School of Environment, Jinan University, Guangzhou 510632, China
Male Wistar rats were exposed to SO2 (7mg/m3) for 28 days, 4 hours per day in SO2 group. Rats in SO2+NALC group were exposed to SO2 and NALC (50mg/kg b.w., i.p.) every other day, which was dissolved in saline. Rats in control group were exposed to filtered air and saline. The mRNA levels of complexes IV and V subunits (CO1 & ATP6) and three mitochondrial transcript factors (PGC1-α, NRF1, TFAM) were analyzed by real-time RT-PCR after exposure. And the protein levels of the above three mitochondrial transcript factors were detected by Western blot. The results showed that the mRNA and protein expression levels of PGC1-α, NRF1and TFAM were decreased significantly after SO2 inhalation, combined with the down-regulations of CO1 & ATP6 on mRNA levels. But NALC could alleviate the depressions of these genes. It indicated that SO2 exposure could impact the transcription of mitochondria DNA through PGC1-α-NRF1-TFAM down-regulation, interfere the synthesis of important components of oxidative phosphorylation. The mechanism might be related to the production of free radicals. Antioxidants could be used to alleviate sulfur dioxide-induced mitochondrial damage in rat hearts.
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