Reactivation of aerobic granular sludge cultivated by low-strength wastewater after room-temperature storage
ZOU Jin-te1, HE Hang-tian2, PAN Ji-yang2, TAO Ya-qiang2, LI Jun1
1. Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou 310014, China;
2. College of Civil Engineering, Zhejiang University of Technology, Hangzhou 310014, China
To investigate the feasibility of room-temperature storage of aerobic granular sludge (AGS) cultivated by real and low-strength wastewater, the variations of AGS characteristics, specific oxygen uptake rate (SOUR), removal efficiency and microbial community were explored. The experimental results show that after 60days storage using tap water at room-temperature, the structure of AGS was still intact and did not disintegrate obviously. The mixed liquor suspended solids (MLSS) slightly decreased from 4960mg/L to 4740mg/L, but the settling property maintained well (SVI:24.2mL/g). The decrease in SOUR was very slight (16%), especially for the SOUR of nitrifying bacteria. The abundance of microbial community changed at the phylum and genus level. After restarting the reactor, AGS morphology recovered quickly and the granule size increased after long-term operation (200~250μm). The sludge settling property has always maintained well (SVI<20mL/g), and the SOUR recovered soon after 20days operation. The COD removal efficiency was completely recovered after 11days operation (around 53mg/L in the effluent). The NH4+-N removal efficiency was also completely recovered after 5 days operation (around 0.7mg/L in the effluent). AGS storage at room-temperature has significant value in practical application due to its convenient operation and fast recovery of reactor stable operation.
邹金特, 何航天, 潘继杨, 陶亚强, 李军. 低碳源废水培养的好氧颗粒污泥常温储存后活性恢复研究[J]. 中国环境科学, 2018, 38(12): 4530-4536.
ZOU Jin-te, HE Hang-tian, PAN Ji-yang, TAO Ya-qiang, LI Jun. Reactivation of aerobic granular sludge cultivated by low-strength wastewater after room-temperature storage. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(12): 4530-4536.
Zhang Q G, Hu J J, Lee D J. Aerobic granular processes:Current research trends. Bioresource Technology, 2016,210:74-80.
[2]
Zou J T, Tao Y Q, Li J, et al. Cultivating aerobic granular sludge in a developed continuous-flow reactorwith two-zone sedimentation tank treating real and low-strength wastewater[J]. Bioresource Technology, 2018,247:776-783.
[3]
Li J, Ding L B, Cai A, et al. Aerobic sludge granulation in a full-scale sequencing batch reactor[J]. Biomedical Research, 2014,Int.268789.
[4]
Pronk M, de Kreuk M K, de Bruin B, et al. Full scale performance of the aerobic granular sludge process for sewage treatment. Water Research, 2015,84,207-217.
Wang X, Zhang H, Yang F, et al. Long-term storage and subsequent reactivation of aerobic granules[J]. Bioresource Technology, 2008,99:8304-8309.
[7]
Gao D, Yuan X, Liang H. Reactivation performance of aerobic granules under different storage strategies[J]. Water Research, 2012, 46(10):3315-3322.
[8]
Hu J, Zhang Q, Chen Y Y, et al. Drying and recovery of aerobic granules[J]. Bioresource Technology, 2016,218:397-401.
[9]
American Public Health Association (APHA), 2005. Standard methods for the examination of water and wastewater, 21th ed[M]. American Public Health Association, washington, dc.
[10]
Liu Y C, Shi H C, Li W L, et al. Inhibition of chemical dose in biological phosphorus and nitrogen removal in simultaneous chemical precipitation for phosphorus removal[J]. Bioresource Technology, 2011,102(5):4008-4012.
[11]
Zeng P, Zhuang W Q, Tay S T L, et al. The influence of storage on the morphology and physiology of phthalic acid-degrading aerobic granules[J]. Chemosphere, 2007,69(11):1751-1757.
[12]
Wang Y Y, Zhou S, Wang H, et al. Comparison of endogenous metabolism during long-term anaerobic starvation of nitrite/nitrate cultivated denitrifying phosphorus removal sludges[J]. Water Research, 2015,68(1):374-386.
[13]
Tay J H, Liu Q S, Liu Y. Characteristics of aerobic granules grown on glucose and acetate in sequential aerobic sludge blanket reactors[J]. Environmental Technology, 2002,23:931-936.
[14]
He Q L, Zhang W, Zhang S L, et al. Performance and microbial population dynamics during stable operation and reactivation after extended idle conditions in an aerobic granular sequencing batch reactor[J]. Bioresource Technology, 2017,238:116-121.
[15]
Morgenroth E, Obermayer A, Arnold E, et al. Effect of long-term idle periods on the performance of sequencing batch reactors[J]. Water Science and Technology, 2000,41:105-113.
[16]
Saeed T, Sun G. Kinetic modelling of nitrogen and organics removal in vertical and horizontal flow wetlands[J]. 2011, Water Research, 2011, 45:3137-3152.
[17]
Saeed T, Sun G. A review on nitrogen and organics removal mechanisms in subsurface flow constructed wetlands:dependency on environmental parameters, operating conditions and supporting media[J]. 2012, Journal of Environmental Management, 112:429-448.
Li E C, Lu S G. Denitrification processes and microbial communities in a sequencing batch reactor treating nanofiltration (NF) concentrate from coking wastewater[J]. Water Science and Technology, 2017, 76(12):3289-3298.
[22]
Wu R N, Meng H, Wang Y F, et al. A more comprehensive community of ammonia-Oxidizing archaea (AOA) revealed by genomic DNA and RNA analyses of amoA gene in subtropical acidic forest soils[J]. Microbial Ecology, 2017,74(4):910-922.
[23]
Kim K K, Lee K C, Eom M K, et al. Variibacter gotjawalensis gen. nov., sp. nov., isolated from soil of a lava forest[J]. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2014,105(5):915-924.
[24]
Albuquerque L, Rainey F A, Nobre M F, et al. Elioraea tepidiphila gen. nov., sp. nov., a slightly thermophilic member of the Alphaproteobacteria[J]. International Journal of Systematic and Evolutionary Microbiology, 2008,58:773-778.
[25]
Lee H J, Lee S H, Lee S S, et al. Ramlibacter solisilvae sp. nov., isolated from forest soil, and emended description of the genus Ramlibacter[J]. International Journal of Systematic and Evolutionary Microbiology, 2014,64:1317-1322.
