1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China;
2. Jiangsu Provincial Key Laboratory of Environmental Science and Engineering, Suzhou 215009, China;
3. Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou 215009, China;
4. Jiangsu Key Laboratory of Anaerobic Biotechnology, Wuxi 214122, China
The UASB continuous flow reactor was used to study the long-term effects of different organic concentrations on the anammox nitrogen removal and microbial community structure. The results showed that the 40mg/L of COD concentration provided the most promotion to anammox reaction among the COD concentration levels with 0, 20, 40, 60 and 80mg/L. At this concentration, the TN and COD removal rates were stabilized at 88.5% and 75.3%, respectively. Under a lower level (20mg/L), the anammox reaction was not affected significantly. However, the nitrogen removal performance was inhibited when the COD concentrations were higher at 60 and 80mg/L. The microbial community structure under different COD concentrations was analyzed by high-throughput sequencing technology. The results indicated that the Chloroflexi, Planctomycetes, Proteobacteria and Actinobacteria were always dominated under different COD concentrations, their relative abundance varied in different scenarios. The relative abundance of Planctomycetes was decreased from 24.60% to 7.70% with the increase of COD concentration (0 to 80mg/L). Among them, Candidatus Brocadia declined fastest, and the abundance was decreased from 12.14% to 3.63%. The relative abundance of Proteobacteria was increased from 15.40% to 36.30%, among which Bdelloribrio had the biggest increase from 0.01% to 8.39%.
黄孝肖,陈重军,张蕊,等.厌氧氨氧化与反硝化耦合反应研究进展[J]. 应用生态学报, 2012,23(3):849-856. Huang X X, Chen C J, Zhang L, et al. Research progress in anammox-denitrification coupling process[J]. Chinese Journal of Applied Ecology, 2012,23(3):849-856.
[2]
Khiewwijit R, Rijnaarts H, Temmink H, et al. Glocal assessment of integrated wastewater treatment and recovery concepts using partial nitritation/Anammox and microalgae for environmental impacts[J]. Science of the Total Environment, 2018,628-629:74-84.
[3]
李海玲,李冬,张杰,等.调控温度和沉降时间实现ANAMMOX颗粒快速启动及其稳定运行[J]. 环境科学, 2019,40(2):837-844. Li H L, Li D, Zhang J, et al. Adjusting temperature and settling time to achieve anammox particles rapid start-up and stable operation[J]. Environmental Science, 2019,40(2):837-844.
[4]
唐崇俭.厌氧氨氧化工艺特性与控制技术的研究[D]. 杭州:浙江大学, 2011. Tang C J. Study on process characteristics and control technology of anammox process[D]. Hangzhou:Zhejiang University, 2011.
[5]
Zhang X J, Zhang H Z, Ye C M, et al. Effect of COD/N ratio on nitrogen removal and microbial communities of CANON process in membrane bioreactors[J]. Bioresource Technology, 2015,189(3):302-308.
[6]
Qin Y J, Cao Y, Ren J Y, et al. Effect of glucose on nitrogen removal and microbial community in anammox-denitrification system[J]. Bioresource Technology, 2017,244:33-39.
[7]
杨朋兵,李祥,黄勇,等.苯酚对厌氧氨氧化污泥脱氮效能长短期影响[J]. 环境科学, 2015,36(10):3771-3777. Yang P B, Li X, Huang Y, et al. Short or long term influence of Phenol on nitrogen removal efficiency of anammox sludge[J]. Environmental Science, 2015,36(10):3771-3777.
[8]
王凡,刘凯,林兴,等.不同TOC/NH4+-N对厌氧氨氧化脱氮效能的影响[J]. 环境科学, 2017,38(8):3415-3421. Wang F, Liu K, Lin X, et al. Effect of different TOC to NH4+-N ratios on nitrogen removal efficiency in the anammox process[J]. Environmental Science, 2017,38(8):3415-3421.
[9]
Van De Graaf A A, De Bruijn P, Robertson LA, et al. Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor[J]. Microbiology, 1996,142(8):2187-2196.
[10]
国家环境保护总局水与废水监测分析方法编委会.水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社, 2002. State Environmental Protection Administration. Determination methods for examination of water and wastewater (4th Ed)[M]. Beijing:China Environmental Science Press, 2002.
[11]
Zhang X L, Tian X Q, Ma L Y, et al. Biodiversity of the symbiotic bacteria associated with toxic marine dinoflagellate[J]. Journal of Biosciences and Medicines, 2015,3(6):23-28.
[12]
Zhang F Z, Peng Y Z, Miao L, et al. A novel simultaneous partial nitrification Anammox and denitrification (SNAD) with intermittent aeration for cost-effective nitrogen removal from mature landfill leachate[J]. Chemical Engineering Journal, 2017,313:619-628.
[13]
吕永涛,陈祯,吴红亚,等.有机物浓度对厌氧氨氧化脱氮性能影响试验研究[J]. 环境工程学报, 2009,3(7):1189-1192. Lv Y T, Chen Z, Wu H Y, et al. Effect of organic substrate on nitrogen removal of ANAMMOX[J]. Journal of Environmental Engineering, 2009,3(7):1189-1192.
[14]
Zheng Z M, Li Y, Li J, et al. Effects of carbon sources, COD/NO2--N ratios and temperature on the nitrogen removal performance of the simultaneous partial nitrification, anammox and denitrification (SNAD) biofilm[J]. Water Science and Technology, 2017,75(7):1712-1721.
[15]
朱葛夫,张净瑞,刘超翔,等.厌氧氨氧化工艺的启动及有机物浓度对其影响研究[J]. 环境工程, 2016,34(2):27-32. Zhu G F, Zhang R J, Liu C X, et al. Study on the start-up of anaerobic ammonia oxidation and the effect of COD concentration[J]. Environmental Engineering, 2016,34(2):27-32.
[16]
管勇杰,于德爽,李津,等.有机碳源作用下厌氧氨氧化系统的脱氮效能[J]. 环境科学, 2017,38(2):654-664. Guan Y J, Yu D S, Li J, et al. Nitrogen removal performance of ANAMMOX with different organic carbon sources[J]. Environmental Science, 2017,38(2):654-664.
[17]
操沈彬,王淑莹,吴程程,等.有机物对厌氧氨氧化系统的冲击影响[J]. 中国环境科学, 2013,33(12):2164-2169. Cao S B, Wang S Y, Wu C C, et al. Shock effect of organic matters on anaerobic ammonia oxidation system[J]. China Environmental Science, 2013,33(12):2164-2169.
[18]
李冬,王艳菊,吕育锋,等.有机碳源对厌氧氨氧化污泥颗粒化的影响[J]. 哈尔滨工业大学学报, 2018,50(9):122-128. Li D, Wang Y J, Lv Y F, et al.Effect of organic carbon source on granulation of anaerobic ammonia oxidation sludge[J]. Journal of Harbin Institute of Technology, 2018,50(9):122-128.
[19]
汪瑶琪,张敏,姜滢,等.厌氧氨氧化启动过程及微生物群落结构特征[J]. 环境科学, 2017,38(12):5184-5191. Wang Y W, Zhang M, Jiang Y, et al.Start-up and characteristics of the microbial community structure of anammox[J]. Environmental Science, 2017,38(12):5184-5191.
[20]
Zhang K, Yang B, Ma Y G, et al. A novel anammox process combined with vibration technology[J]. Bioresource Technology, 2018,256:277-284.
[21]
陈重军,张海芹,汪瑶琪,等.基于高通量测序的ABR厌氧氨氧化反应器各隔室细菌群落特征分析[J]. 环境科学, 2016,37(7):2652-2658. Chen C J, Zhang H Q, Wang Y Q, et al. Characteristics of microbial community in each compartment of ABR anammox reactor based on high-throughput sequencing[J]. Environmental Science, 2016,37(7):2652-2658.
[22]
孙娜.厌氧氨氧化耦合反硝化/亚硝化工艺的研究[D]. 济南:山东大学, 2016. Sun N. The study of integration of anammox and denitrification/partial nitrification process[D]. Jinan:Shandong University, 2016.
[23]
Sheng S X, Liu B, Hou X Y, et al. Effects of different carbon sources and C/N ratios on the simultaneous anammox and denitrification process[J]. International Biodeterioration & Biodegradation, 2018,127:26-34.
[24]
王慧.硫酸盐型厌氧氨氧化同步脱氮除硫实验研究[D]. 沈阳:沈阳工业大学, 2017. Wang H. Study on sulfate-reducing anammox reaction for synchronizing ammonia and sulfate removal[D]. Shenyang:Shenyang University of Technology, 2017.
[25]
Liu W R, Yang D H, Chen W J, et al. High-throughput sequencing-based microbial characterization of size fractionated biomass in an anoxic anammox reactor for low-strength wastewater at low temperatures[J]. Bioresource Technology, 2017,231:45-52.
[26]
Kuenen J G, Jetten M S M. Extraordinary anaerobic ammonium-oxidizing bacteria[J]. ASM news, 2001,67:456-463.
[27]
Schmid M, Twachtmann U, Klein M, et al. Molecular evidence for genus level diversity of bacteria capable of catalyzing anaerobic ammonium oxidation[J]. Systematic and Applied Microbiology, 2000, 23(1):93-106.
[28]
Puyol D, Carvajal-Arroyo J M, Garcia B, et al. Kinetic characterization of Brocadia spp.-dominated anammox cultures[J]. Bioresource Technology, 2013,139(7):94-100.
[29]
齐雨.复合型人工湿地菌群结构及其对氮循环的影响研究[D]. 重庆:重庆大学, 2016. Qi Y. Diversity of bacterial groups in a hybrid constructed wetlandand its impact on the nitrogen cycle[D]. Chongqing:Chongqing University, 2016.
[30]
侯爱月,李军,卞伟,等.不同短程硝化系统中微生物群落结构的对比分析[J]. 中国环境科学, 2016,36(2):428-436. Hou A Y, Li J, Bian W, et al. Analysis of microbial community structure in different partial nitrification system[J]. China Environmental Science, 2016,36(2):428-436.
[31]
王宇佳.亚硝化过程控制与厌氧氨氧化工艺运行及其微生物特性[D]. 沈阳:东北大学, 2017. Wang Y J. Nitritation process control and anammox process performance and their microbial characteristics[D]. Shenyang:Northeastern University, 2017.