Abundance dynamics of β-lactamase genes in penicillin biomass-residue composting
DUAN Hui-ying1, ZHAO Juan1, ZHANG Zhen-hua2, YU Ran1, ZHANG Di-ni2, LIU Yan2, WANG Chang-yong2
1. Department of Energy and Environment Southeast University, Nanjing 210096, China;
2. Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China
In order to reveal the environmental impacts of antibiotic resistance genes (ARGs) during penicillin biomass-residue composting because of the possible antibiotic residues, the relative abundances and distributions of eight typical β-lactamase genes (bla-TEM、bla-CTX-M-1、bla-CTX-M-9、bla-IMP-1、bla-VIM-2、bla-CMY、bla-OXA-23、bla-NDM-1) were investigated with quantitative PCR technique. The results indicated that high temperature composting greatly shortened the degradation time of penicillin. No bla-NDM-1 gene was detected in any sample. The abundances of all studied genes were significantly reduced from day 1 to day 30 in the different penicillin biomass-residue composting experiments except those of the bla-IMP-1 and bla-VIM-2 genes which slight increased. The penicillin residue induced the increases of relative abundance of bla-TEM, bla-CTX-M-1. bla-CTX-M-9, bla-CMY, bla-OXA-23, and bla-VIM-2 genes during the early composting stage. With the elongation of the composting process, penicillin residue gradually degraded. At the end of composting, the relative abundances of bla-TEM、bla-CTX-M-1、bla-CTX-M-9、bla-CMY significantly decreased in all treated samples and the control in comparison with those of bla-IMP-1、bla-VIM-2, which greatly increased.
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