Abundance dynamics of β-lactamase genes in penicillin biomass-residue composting
DUAN Hui-ying1, ZHAO Juan1, ZHANG Zhen-hua2, YU Ran1, ZHANG Di-ni2, LIU Yan2, WANG Chang-yong2
1. Department of Energy and Environment Southeast University, Nanjing 210096, China;
2. Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China
In order to reveal the environmental impacts of antibiotic resistance genes (ARGs) during penicillin biomass-residue composting because of the possible antibiotic residues, the relative abundances and distributions of eight typical β-lactamase genes (bla-TEM、bla-CTX-M-1、bla-CTX-M-9、bla-IMP-1、bla-VIM-2、bla-CMY、bla-OXA-23、bla-NDM-1) were investigated with quantitative PCR technique. The results indicated that high temperature composting greatly shortened the degradation time of penicillin. No bla-NDM-1 gene was detected in any sample. The abundances of all studied genes were significantly reduced from day 1 to day 30 in the different penicillin biomass-residue composting experiments except those of the bla-IMP-1 and bla-VIM-2 genes which slight increased. The penicillin residue induced the increases of relative abundance of bla-TEM, bla-CTX-M-1. bla-CTX-M-9, bla-CMY, bla-OXA-23, and bla-VIM-2 genes during the early composting stage. With the elongation of the composting process, penicillin residue gradually degraded. At the end of composting, the relative abundances of bla-TEM、bla-CTX-M-1、bla-CTX-M-9、bla-CMY significantly decreased in all treated samples and the control in comparison with those of bla-IMP-1、bla-VIM-2, which greatly increased.
Baquero F, Martínez J L, Cantón R. Antibiotics and antibiotic resistance in water environments[J]. Current Opinion in Biotechnology, 2008,19(3):260-265.
[2]
Levy S B, Marshall B. Antibacterial resistance worldwide:causes, challenges and responses[J]. Nature Medicine, 2004,10(12):122-129.
[3]
Colomerlluch M, Imamovic L, Jofre J, et al. Bacteriophages carrying antibiotic resistance genes in fecal waste from cattle, pigs, and poultry[J]. Antimicrobial Agents & Chemotherapy, 2011,55(10):4908-4911.
[4]
Hsu J T, Chen C Y, Young C W, et al.Prevalence of sulfonamideresistant bacteria, resistance genes and integron-associated horizontal gene transfer in natural water bodies and soils adjacent to a swine feedlot in northern Taiwan[J]. J. Hazard Mater., 2014,277(4):34-43.
[5]
Li D, Yu T, Zhang Y, et al.Antibiotic resistance characteristics of environmental bacteria from an oxytetracycline production wastewater treatment plant and the receiving river[J]. Applied & Environmental Microbiology, 2010,76(11):3444-3451.
[6]
Udikovickolic N, Wichmann F, Broderick N A, et al. Bloom of resident antibiotic-resistant bacteria in soil following manure fertilization[J]. Proceedings of the National Academy of Sciences of the United States of America, 2014,111(42):1-6.
[7]
D'Costa V M, King C E, Kalan L, et al. Antibiotic resistance is ancient[J]. Nature, 2011,477(7365):457-461.
[8]
Lang K S, Anderson J M, Schwarz S, et al.Novel florfenicol and chloramphenicol resistance gene discovered in Alaskan soil by using functional metagenomics[J]. Applied & Environmental Microbiology Aem., 2010,76(15):5321-5326.
[9]
Zhang Z, Zhao J, Yu C, et al.Evaluation of aerobic co-composting of penicillin fermentation fungi residue with pig manure on penicillin degradation, microbial population dynamics and composting maturity[J]. Bioresource Technology, 2015,198(8):403-409.
Wang L, Gutek A, Grewal S, et al. Changes in diversity of cultured bacteria resistant to erythromycin and tetracycline in swine manure during simulated composting and lagoon storage[J]. Letters in Applied Microbiology, 2015,61(3):245-251.
Ma S, Liu Y, Yu R, et al. Determination of sodium penicillin in soil through accelerated solvent extraction and solid phase extration followed by high performance liquid chromatography[J]. Environmental Chemistry, 2014(11):1978-1985.
[14]
Colomerlluch M, Jofre J, Muniesa M. Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samples[J]. PLoS One, 2011,6(3):17549.
[15]
Wendel A F, Brodner A H B, Wydra S, et al. Genetic characterization and emergence of the metallo-β-lactamase GIM-1 in Pseudomonas spp. and Enterobacteriaceae during a Long-Term outbreak[J]. Antimicrobial Agents & Chemotherapy, 2013,57(10):5162-5165.
[16]
Krüttgen A, Razavi S, Imöhl M, et al.Real-time PCR assay and a synthetic positive control for the rapid and sensitive detection of the emerging resistance gene New Delhi Metallo-β-lactamase-1(bla(NDM-1))[J]. Medical Microbiology & Immunology, 2011,200(2):137-141.
[17]
Geyer C N, Reisbig M D, Hanson N D. Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated amp C β-lactamase genes[J]. J. Clin. Microbiol., 2012,50(11):3722-3725.
[18]
Huang X Z, Cash D M, Chahine M A, et al. Development and validation of a multiplex TaqMan real-time PCR for rapid detection of genes encoding four types of class D carbapenemase in Acinetobacter baumannii[J]. Journal of Medical Microbiology, 2012,61(11):1532-1537.
Paterson D L, Bonomo R A. Extended-spectrum beta-lactamases:a clinical update[J]. Clinical Microbiology Reviews, 2005, 18(4):657-686.
[23]
Zhou S, Nikolausz M, Zhang J, et al. Variation of the microbial community in thermophilic anaerobic digestion of pig manure mixed with different ratios of rice straw[J]. Journal of Bioscience & Bioengineering, 2016,122(3):334-340.
[24]
Song C, Li M, Jia X, et al. Comparison of bacterial community structure and dynamics during the thermophilic composting of different types of solid wastes:anaerobic digestion residue, pig manure and chicken manure[J]. Microb. Biotechnol., 2014, 7(5):424-433.
[25]
Sarria J C. Infections caused by kluyvera species in humans[J]. Clinical Infectious Diseases, 2001,33(7):69-74.
[26]
Zhao W H, Hu Z Q. Epidemiology and genetics of CTX-M extended-spectrum β-lactamases in Gram-negative bacteria[J]. Critical Reviews in Microbiology, 2013,39(1):71-72.
[27]
D'Andrea M M, Arena F, Pallecchi L, et al. CTX-M-type β-lactamases:a successful story of antibiotic resistance[J]. International Journal of Medical Microbiology Ijmm, 2013, 303(6/7):305-317.
[28]
Osano E, Arakawa Y, Wacharotayankun R, et al. Molecular characterization of an enterobacterial metallo beta-lactamase found in a clinical isolate of Serratia marcescens that shows imipenem resistance[J]. Antimicrobial Agents & Chemotherapy, 1994,38(1):71-78.
[29]
Widmann M, Pleiss J.Protein variants form a system of networks:Microdiversity of IMP metallo-beta-lactamases[J]. PLoS One, 2014,9(7):101813.
[30]
Zhao Z, Wang J, Han Y, et al.Nutrients, heavy metals and microbial communities co-driven distribution of antibiotic resistance genes in adjacent environment of mariculture[J]. Environmental Pollution, 2016,220:909-918.
[31]
Su J Q, Wei B, Ouyang W Y, et al. Antibiotic resistome and its association with bacterial communities during sewage sludge composting[J]. Environmental Science & Technology, 2015,49(12):7356-7363.
[32]
Lauretti L, Riccio M L, Mazzariol A, et al. Cloning and characterization of blaVIM, a new integron-borne metallo-betalactamase gene from a Pseudomonas aeruginosa clinical isolate[J]. Antimicrobial Agents & Chemotherapy, 1999,43(7):1584-1590.
[33]
Fischer J, San J M, Roschanski N, et al. Spread and persistence of VIM-1 Carbapenemase-producing Enterobacteriaceae in three German swine farms in 2011 and 2012[J]. Veterinary Microbiology, 2016,200:118-123.
[34]
Falgenhauer L, Ghosh H, Guerra B, et al. Comparative genome analysis of IncHI2VIM-1carbapenemase-encoding plasmids of Escherichia coli and Salmonella enterica isolated from a livestock farm in Germany[J]. Veterinary Microbiology, 2015,45(5):609-624.
[35]
Jacoby G A. AmpC beta-lactamases[J]. Clinical Microbiology Reviews, 2009,22(1):161-182.
[36]
Endimiani A, Hilty M, Perreten V. CMY-2-producing Escherichia coli in the nose of pigs[J]. Antimicrobial Agents & Chemotherapy, 2012,56(8):4556-4557.
[37]
Mataseje L F, Baudry PJZhanel G G, Morck D W, et al. Comparison of CMY-2plasmids isolated from human, animal, and environmental Escherichia coli and Salmonella spp. from Canada[J]. Diagnostic Microbiology & Infectious Disease, 2010,67(4):387-391.
[38]
Avison M B, Bennett P M, Walsh T R. Beta-lactamase expression in Plesiomonas shigelloides[J]. Journal of Antimicrobial Chemotherapy, 2000,45(6):877-880.
[39]
Poirel L, Nordmann P. Carbapenem resistance in Acinetobacter baumannii:Mechanisms and epidemiology[J]. Clinical Microbiology and Infection, 2006,12(9):826-836.
