Combined remediation of tribenuron methyl-contaminated soil by Methylopila sp. SD-1 and soybean
ZHANG Hao1, LI Na1, HUA Zi-wei1, NIU Qiu-hong1, CHEN Zhao-jin2, HAN Hui2, DONG Wei-liang3
1. School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China;
2. School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China;
3. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China
One highly efficient tribenuron methyl-degrading strain, named SD-1, was isolated. According to the phenotypic, physiological and biochemical characteristics, combined with 16S rRNA gene sequence analysis, the strain SD-1 was identified as Methylopila sp. SD-1, which was the first reported strain in the genus of Methylopila that could degrade tribenuron methyl. The strain SD-1 completely degraded 50mg/L of tribenuron methyl in 4d. The optimum temperature and pH were 30℃ and 7.0, respectively. The toxicity of the intermediate from the degradation of tribenuron methyl to soybean growth was significantly reduced. Soybean root exudates proved to promote the growth of strain SD-1, the number of strain SD-1increased from 1.0×107CFU/mL to 6.7×107CFU/mL after 5d. Root exudates contained 16 kinds of amino acids, and the strain SD-1 showed chemotaxis response towards Asp, Glu and Phe in root exudates. The suspension of strain SD-1 was inoculated and the soybean was planted in the soil polluted by tribenuron methyl (3mg/kg). After 4days, strain SD-1 colonized on the root of soybean depending on chemotaxis, and the survival rate of SD-1 was increased. Compared with the treatments that didn't plant soybean, the degradation rates of tribenuron methyl by strain SD-1 in the soybean rhizosphere soil were increased by 36.0%.
顾林玲,王欣欣.全球除草剂市场、发展概况及趋势[J]. 现代农药, 2016,15(2):8-12. Gu L L, Wang X X. The global market, development, trend of herbicide[J]. Modern Agrochemicals, 2016,15(2):8-12.
[2]
Luo X W, Zhang D Y, Zhu T H, et al. Adaptation mechanism and tolerance of Rhodopseudomonas palustris PSB-S under pyrazosulfuron-ethyl stress[J]. BMC Microbiology, 2018,18(1):207-218.
[3]
Wang N X, Tang Q, Ai G M, et al. Biodegradation of tribenuron methyl that is mediated by microbial acidohydrolysis at cell-soil interface[J]. Chemosphere, 2012,86(11):1098-1105.
[4]
Zhang J J, Chen Y F, Fang T, et al. Co-metabolic degradation of tribenuron methyl, a sulfonylurea herbicide by Pseudomonas sp. strain NyZ42[J]. International Biodeterioration and Biodegradation, 2013, 76:36-40.
[5]
王惟帅,吴济南,张天宝,等.苯磺隆降解菌生长条件研究[J]. 山西农业科学, 2012,40(8):862-865. Wang W S, Wu J N, Zhang T B, et al. Study on growth conditions of tribenuron-methyl degradation strain[J]. Journal of Shanxi Agricultural Sciences, 2012,40(8):862-865.
[6]
田爽,田方方,王晓萍.4株苯磺隆降解菌的分离鉴定及其生长特性[J]. 农业资源与环境学报, 2014,31(5):437-445. Tian S, Tian F F, Wang X P. Isolation, identification and growth characteristics of four tribenuron-methyl degrading bacterias[J]. Journal of Agricultural Resources and Environment, 2014,31(5):437-445.
[7]
赵永斌,霍德敏,张天宝,等.苯磺隆降解菌的分离鉴定和特性研究[J]. 核农学报, 2015,29(1):202-207. Zhao Y B, Huo D M, Zhang T B, et al. Isolation, identification and characterization of degradation bacteria of tribenuron methyl[J]. Journal of Nuclear Agricultural Sciences, 2015,29(1):202-207.
[8]
Song J, Gu J, Zhai Y, et al. Biodegradation of nicosulfuron by a Talaromyces flavus LZM1[J]. Bioresource Technology, 2013,140:243-248.
[9]
Vicente V P, Carlos O, Aurelio G C, et al. Root exudates:from plant to rhizosphere and beyond[J]. Plant Cell Reports, 2020,39(11):3-17.
[10]
王慧,胡金星,秦智慧,等.细菌对有机污染物的趋化性及其对降解的影响[J]. 浙江大学学报(农业与生命科学版), 2017,43(6):676-684. Wang H, Hu J X, Qin Z H, et al. Bacterial chemotaxis to organic pollutants and its influence on biodegradation[J]. Journal of Zhejiang University (Agriculture and Life Science), 2017,43(6):676-684.
[11]
Wu L, Wu H J, Qiao J Q, et al. Novel routes for improving biocontrol activity of Bacillus based bioinoculants[J]. Frontiers in Microbiology, 2015,6:1395-1408.
[12]
Zhang H, Chen F, Zhao H J, et al. Colonization on cucumber root and enhancement of chlorimuron-ethyl degradation in rhizosphere by Hansschlegelia zhihuaiae S113 and root exudates[J]. Journal of Agricultural and Food Chemistry, 2018,66(18):4584-4591.
[13]
Marchesi J R, Sato T, Weightman A J, et al. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA[J]. Applied and Environmental Microbiology, 1998,64(2):795-799.
[14]
Tamura K, Peterson D, Peterson N, et al. MEGA5:Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J]. Molecular Biology and Evolution, 2011,28(10):2731-2739.
[15]
东秀珠,蔡妙英.常见细菌系统鉴定手册[M]. 北京:科学出版社, 2001:349-418. Dong X Z, Cai M Y. Manual for systematic identification of common bacteria[M]. Beijing:Science Press, 2001:349-418.
[16]
Whitman W B. Systematic Bacteriology[J]. Asian Australasian Journal of Animal Sciences, 2013,26(9):1237-1246.
[17]
侯颖,李静泉,尤晓颜,等.精喹禾灵降解菌株Bacillus subtilis H的分离鉴定及降解特性[J]. 中国环境科学, 2018,38(4):1466-1472. Hou Y, Li J Q, You X Y, et al. Isolation and identification of quizalofop-p-ethyl-degrading strain Bacillus subtilis H and its degradation characteristics[J]. China Environmental Science, 2018, 38(4):1466-1472.
[18]
Huang G, Guo G, Yao S, et al. Organic acids, amino acids compositions in the root exudates and Cu-accumulation in Castor (Ricinus communis L.) under Cu stress[J]. International Journal of Phytoremediation, 2015,18(1):33-40.
[19]
Adler J. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli [J]. Journal of General Microbiology, 1973,74(1):77-91.
[20]
Peng T, Wu J, Liu H, et al. Correction:Assimilable organic carbon (AOC) determination using GFP-tagged Pseudomonas fluorescens P-17in water by flow cytometry[J]. PloS ONE, 2018,13(12):e0209721.
[21]
Zhang Y P, Zhang Q P, Li S H, et al. Simultaneous determination of neonicotinoids and fipronils in tea using a modified QuEChERS method and liquid chromatography-high resolution mass spectrometry[J]. Food Chemistry, 2020,doi:10.1016/j.foodchem.2020.127159.
[22]
Huang X, He J, Sun J, et al. Isolation and characterization of a metsulfuron-methyl degrading bacterium Methylopila sp. S113[J]. International Biodeterioration and Biodegradation, 2007,60(3):152-158.
[23]
Wen Y, Huang X, Zhou Y, et al. Hansschlegelia zhihuaiae sp. nov., isolated from a polluted farmland soil[J]. International Journal of Systematic and Evolutionary Microbiology, 2011,61(5):1114-1117.
[24]
Hang B J, Hong Q, Xie X T, et al. SulE, a sulfonylurea herbicide de-esterification esterase from Hansschlegelia zhihuaiae S113[J]. Applied and Environmental Microbiology, 2012,78(6):1962-1968.
[25]
Yang L, Li X, Li X, et al. Bioremediation of chlorimuron-ethyl-contaminated soil by Hansschlegelia sp. strain CHL1and the changes of indigenous microbial population and N-cycling function genes during the bioremediation process[J]. Journal of Hazardous Materials, 2014,274(15):314-321.
[26]
Yang L Q, Liu L, Salam N, et al. Chenggangzhangella methanolivorans gen. nov., sp. nov., a member of the family Methylocystaceae, transfer of Methylopila helvetica Doronina et al. 2000to Albibacter helveticus comb. nov. and emended description of the genus Albibacter [J]. International Journal of Systematic and Evolutionary Microbiology, 2016,66(8):2825-2830.
[27]
Xiong M, Hu Z, Zhang Y, et al. Survival of GFP-tagged Rhodococcus sp. D310-1in chlorimuron-ethyl-contaminated soil and its effects on the indigenous microbial community[J]. Journal of Hazardous Materials, 2013,252-253(15):347-354.
[28]
Cavaglieri L, Orlando J, Rodríguez M I, et al. Biocontrol of Bacillus subtilis against Fusarium verticillioides in vitro and at the maize root level[J]. Research in Microbiology, 2005,156(5/6):748-754.
[29]
吴林坤,林向民,林文雄.根系分泌物介导下植物-土壤-微生物互作关系研究进展与展望[J]. 植物生态学报, 2014,38(3):298-310. Wu L K, Lin X M, Lin W X. Advances and perspective in research on plant-soil-microbe interactions mediated by root exudates[J]. Chinese Journal of Plant Ecology, 2014,38(3):298-310.
[30]
Ling N, Raza W, Ma J, et al. Identification and role of organic acids in watermelon root exudates for recruiting Paenibacillus polymyxa SQR-21in the rhizosphere[J]. European Journal of Soil Biology, 2011,47(6):374-379.
[31]
Rudrappa T, Czymmek K J, Paré P W, et al. Root-secreted malic acid recruits beneficial soil bacteria[J]. Plant Physiology, 2008,148(3):1547-1556.
[32]
颜朗,张义正,方志荣,等.不同马铃薯基因型对根际细菌群落结构的影响[J]. 四川大学学报(自然科学版), 2020,57(2):383-390. Yan L, Zhang Z Y, Fang Z R, et al. Effects of potato genotype on rhizosphere bacterial community structure[J]. Journal of Sichuan University (Natural Science Edition), 2020,57(2):383-390.
[33]
Xu X H, Zarecki R, Medina S, et al. Modeling microbial communities from atrazine contaminated soils promotes the development of biostimulation solutions[J]. ISME Journal, 2019,13(2):494-508.
[34]
Hou J Y, Liu W X, Wu L H, et al. Rhodococcus sp. NSX2 modulates the phytoremediation efficiency of a trace metal-contaminated soil by reshaping the rhizosphere microbiome[J]. Applied Soil Ecology, 2019,133:62-69.
