Effects and mechanisms of urease-producing bacteria mineralization on remediation of Cd- and Pb-contaminated soil
CAI Hong1, WANG Xiao-yu1, HAN Hui1,2
1. Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China;
2. Henan Provincal Academician Workstation of Water Security for Water Source Region of Mid-line of South-to-North Diversion Project, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China
In order to repair heavy metal contaminated soil and ensure the quality and safety of vegetables, the urease-producing bacteria were screened from the vegetable rhizosphere soil by urease-producing medium. The adsorption and immobilization of Cd and Pb by functional strains were studied. Effects of functional strains on the growth and absorption of Cd and Pb by different vegetables were also studied by hydroponic experiment. Ten urease-producing bacteria were isolated from the rhizosphere soil of lettuce. Two strains, Microbacterium foliorum CH6 and Bacillus thuringiensis N3, showed highly ability to producte urease (62.6 and 59.6 (mS/(cm·min), respectively) and immobilize Cd and Pb (84.6%~91.3%). SEM-EDS, FITR and X-ray showed that CH6and N3reduced the content of Cd and Pb in the solution through cell wall adsorption and induced precipitation. Soil static test showed that strains CH6 and N3 reduced the exchangeable contents of Cd and Pb (46.3%~66.7%) and increased the carbonate bound and residual fraction contents of Cd and Pb (40%~95.4%) in soil. A hydroponic experiment showed that, strains CH6 and N3 significantly reduced the Cd (39.2%~81.4%) and Pb (27.4%~82.4%) contents in the edible parts of lettuce, Chinese cabbage and Ipomoea aquatic. Urease-producing bacteria CH6 and N3 could mineralize CD and Pb in soil, reduce the content of available heavy metals in soil, and inhibit the absorption of Cd and Pb by lettuce, Shanghai green and cabbage.
蔡红, 王晓宇, 韩辉. 产脲酶细菌矿化修复Cd和Pb污染土壤效应和机制[J]. 中国环境科学, 2020, 40(11): 4883-4892.
CAI Hong, WANG Xiao-yu, HAN Hui. Effects and mechanisms of urease-producing bacteria mineralization on remediation of Cd- and Pb-contaminated soil. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(11): 4883-4892.
Kim S W, Chae Y, Moon J, et al. In situ evaluation of crop productivity and bioaccumulation of heavy metals in paddy soils after remediation of metal-contaminated soils[J]. Journal of Agricultural and Food Chemistry, 2017,65(6):1239-1246.
[2]
余志,陈凤,张军方,等.锌冶炼区菜地土壤和蔬菜重金属污染状况及风险评价[J]. 中国环境科学, 2019,39(5):2086-2094. Yu Z, Chen F, Zhang J F, et al. Contamination and risk of heavy metals in soils and vegetables from zinc smelting area.[J]. China Environmental Science, 2019,39(5):2086-2094.
[3]
Franca F, Albuuerque A M A, Almeida A C, et al. Heavy metals deposited in the culture of lettuce (Lactuca sativa L.) by the influence of vehicular traffic in Pernambuco, Brazil[J]. Food Chemistry, 2017, 215:171-176.
[4]
León-Cañedo J A, Alarcón-Silvas S G, Fierro-Sañudo J F, et al. Mercury and other trace metals in lettuce (Lactuca sativa) grown with two low-salinity shrimp effluents:accumulation and human health risk assessment[J]. Science of the Total Environment, 2019,650:2535-2544.
[5]
靳琪,高红,岳波,等.村镇生活垃圾重金属含量及其来源分析[J]. 环境科学, 2018,39(9):4385-4392. Jin Q, Gao H, Yue B, et al. Heavy metal content of rural living solid waste and related source and distribution analysis[J]. Environmental Science, 2018,39(9):4385-4392.
[6]
曾希柏,徐建明,黄巧云,等.中国农田重金属问题的若干思考[J]. 土壤学报, 2013,50(1):186-195. Zeng X B, Xu J M, Huang Q Y, et al. Some thoughts on heavy metals in farmland in China[J]. Acta Pedologica Sinica, 2013,50(1):186-195.
[7]
Chen H, Zhang J, Tang L, et al. Enhanced Pb immobilization via the combination of biochar and phosphate solubilizing bacteria[J]. Environment International, 2019,127:395-401.
[8]
Etesami H. Bacterial mediated alleviation of heavy metal stress and decreased accumulation of metals in plant tissues:Mechanisms and future prospects[J]. Ecotoxicology and Environmental Safety, 2018, 147:175-191.
[9]
余斐,苏艳,李吉跃,等.植物根际促生菌促生机理研究[J]. 林业与环境科学, 2017,33(2):107-112. Yu F, Su Y, Li J Y, et al. Plant growth-promoting rhizobacteria promoting mechanism research[J]. Forestry and Environmental Science, 2017,33(2):107-112.
[10]
韩辉,王晓宇,蔡红,等.重金属固定植物促生细菌的筛选及其阻控小麦富集重金属效应[J]. 环境科学, 2019,40(7),381-388. Han H, Wang X Y, Cai H, et al. Isolation of heavy metal immobilizing and plant growth-promoting bacteria and its effects on reducing heavy metal accumulation in wheat[J]. Environmental Science, 2019,40(7):381-388.
[11]
Wang Q, Chen L, He L Y, et al. Increased biomass and reduced heavy metal accumulation of edible tissues of vegetable crops in the presence of plant growth-promoting Neorhizobium huautlense T1-17and biochar[J]. Agriculture Ecosystems & Environment, 2016,228:9-18.
[12]
王茂林,吴世军,杨永强,等.微生物诱导碳酸盐沉淀及其在固定重金属领域的应用进展[J]. 环境科学研究, 2018,31(2):206-214. Wang M L, Wu S J, Yang Y Q, et al. Microbial induced carbonate precipitation and its application for immobilization of heavy metals:a review[J]. Research of Environmental Science, 2018,31(2):206-214.
[13]
Achal V, Pan X L, Zhang D Y, et al. Bioremediation of Pb-contaminated soil based on microbially induced calcite precipitation[J]. Journal of Microbiology and Biotechnology, 2012,22(2):244-247.
[14]
鲍士旦.土壤农化分析[M]. 3版.北京:中国农业出版社, 2008:86-115. Bao Shidan. Agrochemical analysis of soil[M]. 3rd ed. Beijing:China Agriculture Press, 2008:86-115.
[15]
GB15618-2018土壤环境质量农用地土壤污染风险管控标准(试行)[S]. GB15618-2018 Soil environmental quality, agricultural land, soil pollution risk management and control standards (on trial)[S].
[16]
Omoregie A I, Khoshdelnezamiha G, Senian N, et al. Experimental optimisation of various cultural conditions on urease activity for isolated sporosarcina pasteurii strains and evaluation of their biocement potentials[J]. Ecological Engineering, 2017,109:65-75.
[17]
刘瑞,于章龙,薛冲,等.市售豆芽携带细菌种属鉴定及酸性电解水的杀菌效果[J]. 食品科学, 2017,38(17):168-173. Liu R, Yu Z L, Xue C, et al. Identification of bacterial species and microbial inactivation by acidic electrolyzed water on commercial bean sprouts[J]. Food Science, 2017,38(17):168-173.
[18]
Tessier A, Campbell P G C, Bisson M. et al. Sequential extyaction procedure for the speciation of particulate trace metals[J]. Analytical Chemistry, 1979,51:844-851.
[19]
韩辉,蔡红,王晓宇,等.产多胺细菌的筛选及其阻控空心菜富集Cd和Pb效应[J]. 中国环境科学, 2020,40(6):2692-2699. Han H, Cai H, Wang X Y, et al. Screening of polyamine-producing bacteria and their effects on reducing Cd and Pb accumulation in Ipomoea aquatica[J]. China Environmental Science, 2020,40(6):2692-2699.
[20]
Huang J H, Yuan F, Zeng G M, et al. Influence of pH on heavy metal speciation and removel from wastewater using micellar-enchanced ultrafiltration[J]. Chemosphere, 2017,173:199-206.
[21]
燕传明,贺卓,葛占标,等.两株重金属抗性细菌对铅镉吸附特性的比较研究[J]. 环境科学学报, 2018,38(9):3597-3604. Yan C M, He Z, Ge Z B, et al. Comparative study on adsorption characteristics of lead and cadmium by two heavy metal resistant bacteria[J]. Acta Scientiae Circumstantiae, 2018,38(9):3597-3604.
[22]
Bai J, Yang X, Du R, et al. Biosorption mechanisms involved in immobilization of soil Pb by Bacillus subtilis DBM in a multi-metal-contaminated soil[J]. Journal of Environmental Sciences, 2014,26:2056-2064.
[23]
Wang J, Li Q, Li M M, et al. Competitive adsorption of heavy metal by extracellular polymeric substances (EPS) extracted from sulfate reducing bacteria[J]. Bioresource technology, 2014,163:374-376.
[24]
Pan X H, Chen Z, Chen Y J, et al. The analysis of the immobilization mechanism of Ni(Ⅱ) on Bacillus cereus [J]. Journal of Nanoscience and Nanotechnology, 2011,11(4):3597-3603.
[25]
Mota R, Rossi F, Andrenelli L, et al. Released polysaccharides (RPS) from Cyanothece sp. CCY 0110 as biosorbent for heavy metals bioremediation:interactions between metals and RPS binding sites[J]. Applied Microbiology and Biotechnology, 2016,100:7765-7775.
[26]
Kiran M G, Pakshirajan K, Das G, et al. Heavy metal removal from multicomponent system by sulfate reducing bacteria:Mechanism and cell surface characterization[J]. Journal of Hazardous Materials, 2017,324:62-70.
[27]
Osman D, Waldron K J, Denton H, et al. Copper homeostasis in Salmonella is atypical and copper-CueP is a major periplasmic metal complex[J]. Journal of Biologal Chemistry, 2010,33:25259-25268.
[28]
Lu W B, Shi J J, Wang C H, et al. Biosorption of lead, copper and cadmium by an indigenous isolate Enterobacter, sp. J1possessing high heavy-metal resistance[J]. Journal of Hazardous Materials, 2006, 134:80-86.
[29]
Xu C, He S, Liu Y, et al. Bioadsorption and biostabilization of cadmium by Enterobacter cloacae TU[J]. Chemosphere, 2017, 173:622-629.
[30]
Rizwan M, Ali S, Abbas T, et al. Cadmium minimization in wheat:A critical review[J]. Ecotoxicology and Environmental Safety, 2016,130:43-53.
[31]
Fan S K, Fang X Z, Guan M Y, et al. Exogenous abscisic acid application decrease cadmium accumulation in Arabidopsis plants, which is associated with the inhibition of IRT1-mediated cadmium uptake[J]. Frontier in Plant Science, 2014,5:721.
[32]
Rehman M Z U, Rizwan M, Hussain A, et al. Alleviation of cadmium (Cd) toxicity and minimizing its uptake in wheat (Triticum aestivum) by using organic carbon sources in Cd-spiked soil[J]. Environmental Pollution, 2018,241:557-565.
[33]
冉洪珍,郭朝晖,肖细元,等.改良剂连续施用对农田水稻Cd吸收的影响[J]. 中国环境科学, 2019,39(3):1117-1123. Ran H Z, Guo Z H, Xiao X Y, et al. Effects of continuous application of soil amendments on cadmium availability in paddy soil and uptake by rice[J]. China Environmental Science, 2019,39(3):1117-1123.
[34]
Udeigwe T K, Eze P N, Teboh J M, et al. Application, chemistry, and environmental implications of contaminant-immobilization amendments on agricultural soil and water quality[J]. Environment International, 2011,37(1):258-267.
[35]
Han H, Wang Q, He L Y, et al. Increased biomass and reduced rapeseed Cd accumulation of oilseed rape in the presence of Cd-immobilizing and polyamine-producing bacteria[J]. Journal of Hazardous Materials, 2018,353:280-289.
[36]
王铁军,苏楠楠,雷鹏,等.重金属污染农田生菜根际重金属固定细菌群落组成及其阻控效应[J]. 环境科学, 2019,40(11):5133-5141. Wang T J, Su N N, Lei P, et al. Community structure of the heavy metal immobilized bacteria in the letture (Lactuca sativa L.) rhizosphere soil polluted by heavy metals and its effects on reducing heavy metal accumulation in Letture[J]. Environmental Science, 2019,40(11):5133-5141.
[37]
Li Y, Pang H D, He L Y, et al. Cd immobilization and reduced tissue Cd accumulation of rice (Oryza sativa wuyun-23) in the presence of heavy metal-resistant bacteria[J]. Ecotoxicology and Environmental Safety, 2017,138:56-63.
[38]
Jalilvand N, Akhgar A, Alikhani H A, et al. Removal of heavy metals zinc, lead, and cadmium by biomineralization of urease-producing bacteria isolated from Iranian mine calcareous soils[J]. Journal of Soil Science and Plant Nutrition, 2020, https://doi.org/10.1007/s42729-019-00121-z.
[39]
Huang J H, Yuan F, Zeng G M, et al. Influence of pH on heavy metal speciation and removel from wastewater using micellar-enchanced ultrafiltration[J]. Chemosphere, 2017,173:199-206.
[40]
Zhao X Q, Wang M, Wang H, et al. Study on the remediation of Cd pollution by the biomineralization of urease-producing bacteria[J]. International Journal of Environmental Researh and Public Health, 2019,16(2):14.
[41]
王茂林,吴世军,杨永强,等.微生物诱导碳酸盐沉淀及其在固定重金属领域的应用进展[J]. 环境科学研究, 2018,31(2):206-214. Wang M L, Wu S J, Yang R Q, et al. Microbial induced carbonate precipitation and its application for immobilization of heavy metals:a review[J]. Research of Environmental Sciences, 2018,31(2):206-214.
