NO3-对二级出水DOM与Cu2+络合作用的光谱特性影响

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摘要

利用三维荧光光谱法和紫外分光光度法，研究NO₃^-对二级出水DOM与Cu²⁺络合作用的光谱特性和络合能力的影响.随投加NO₃^-浓度增加，二级出水DOM与Cu²⁺络合作用后代表类蛋白质（类色氨酸）物质荧光峰消失，紫外吸收峰右侧红移.投加NO₃^-浓度与二级出水DOM和Cu²⁺络合作用后的HIX、α₃₀₀、α₂₅₀/α₃₆₅、E₃/E₄和UV₂₅₃/UV₂₀₃值呈显著正相关，与BIX显著负相关（r为-0.958~-0.719），与S₂₄₀_-400和S₂₇₅_-295值分别呈指数增加和指数减少关系（R²分别为0.9678~0.9952和0.9965~0.9978），对FI、α₃₅₀、α₃₅₅和S₃₅₀_-400值影响较小.二级出水DOM中的紫外光类腐殖质、可见光类腐殖质和高激发波长类色氨酸物质与Cu²⁺作用后的条件稳定常数lgK分别为2.08~2.46，1.84~2.51，2.27~2.57L/mmol，配位荧光官能团比例分别为19.5%~36.5%、22.5%~33.3%和24.4%~29.2%.二级出水DOM中类腐殖质和类蛋白质与Cu²⁺的络合反应强度相差不大，且样品中NO₃^-浓度不同时，用部分荧光参数（HIX）和紫外参数（α₃₀₀、α₂₅₀/α₃₆₅、E₃/E₄、S₂₄₀_-400和S₂₇₅_-295）表示DOM的含量、分子性质存在一定的不确定性.

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关键词 ： NO₃^-, 二级出水, DOM, Cu²⁺, 光谱特性

Abstract：

The effects of nitrate on the spectral characteristics and complexation ability of DOM with Cu²⁺ in the secondary effluent of municipal wastewater were investigated by using three-dimensional fluorescence and UV spectrophotometry. The fluorescence peak representing the protein-like (tryptophan-like) produced by complexation of DOM and Cu²⁺ disappeared with the increase of NO₃^- concentration, and the right side of the UV absorption peak occurred red-shift. The concentration of NO₃^- was significantly positively correlated with the values of HIX, α₃₀₀, α₂₅₀/α₃₆₅, E₃/E₄ and UV₂₅₃/UV₂₀₃ after complexation of DOM with Cu²⁺, while negatively correlated with BIX (r with -0.958~-0.719). It had an exponential increase with S₂₄₀_-400 (R² with 0.9678~0.9952) and an exponential decrease with S₂₇₅_-295 (R² with 0.9965~0.9978), but had little effect on the values of FI, α₃₅₀, α₃₅₅ and S₃₅₀_-400. The conditional stability constant lgK of ultraviolet humus-like, visible humus-like and high excitation wavelength tryptophan-like in the secondary effluent of DOM with Cu²⁺ were 2.08~2.46L/mmol, 1.84~2.51L/mmol and 2.27~2.57L/mmol, respectively; and the proportion of coordination fluorescent functional groups were 19.5%~36.5%, 22.5%~33.3% and 24.4%~29.2%, respectively. In summary, there was not significant difference in the intensity of the complexation reaction between Cu²⁺ and humus-like or protein-like from DOM of the secondary effluent. When the NO₃^- concentration in the samples was different, there were some uncertainties in expressing the content of DOM and molecular properties with partial fluorescence (HIX) and ultraviolet parameters (α₃₀₀, α₂₅₀/α₃₆₅, E₃/E₄, S₂₄₀_-400 and S₂₇₅_-295).

Key words： nitrate secondary effluent DOM copper ion spectral characteristic

收稿日期: 2020-04-03

PACS:

X132

基金资助:

国家自然科学基金项目（21407119）；陕西省教育厅重点实验室科研计划项目（18JS055）；西安建筑科技大学预研基金项目（ZR18032）

通讯作者: 杨毅,副教授,yangyi@xauat.edu.cn E-mail: yangyi@xauat.edu.cn

作者简介: 杨毅(1976),女,辽宁铁岭人,副教授,博士,主要从事环境中溶解性有机物的特性研究.发表论文20余篇.

引用本文:

杨毅, 李亚飞, 郑振泽, 张亚楠, 韩丽媛, 徐会宁. NO₃^-对二级出水DOM与Cu²⁺络合作用的光谱特性影响[J]. 中国环境科学, 2020, 40(11): 4982-4988. YANG Yi, LI Ya-fei, ZHENG Zhen-ze, ZHANG Ya-nan, HAN Li-yuan, XU Hui-ning. Effect of NO₃^- on spectral characteristic of the complexation of DOM and Cu²⁺ from secondary effluent. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(11): 4982-4988.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2020/V40/I11/4982

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