Abstract：Considering that limitation that cumbersome process, time-consuming and lagging detection of current activated sludge testing process, a rapid detection of beam deflection method were provided in this study. The polyvinyl alcohol gel beads with a particle size of 4mm and microporous structure of 20μm were put in the printing and dyeing wastewater complete mixed activated sludge treatment system with a sludge load of 0.33KgCOD/(kg MLSS·d) and a hydraulic retention time of 15h. The light spot analyzer was used to detecte light deflection which reflect the surface concentration gradient changes due to the exchange of external solutions and microbial metabolites after the beads reached a stable state. The corresponding COD of effluent after 15h were also measured, meanwhile the COD removal rate were calculated. After ten consecutive months of testing, it was found that the predominant microorganism is bacteria in the PVA-gel beads. The light deflection value of the beads increased from 229.51μm to 299.97μm when the influent COD increased from 91.95mg/L to 519.4mg/L, and The COD removal rate also increased from 16.03% to 66.99%. The light deflection of the beads increased with dissolved oxygen concentration and reached to the higest value 309.3μm at DO=4mg/L, meanwhile, the corresponding COD removal rate reached to the maximum which is 61.18%, when the dissolved oxygen concentration range from 1.5mg/L to 5mg/L. The light deflection of the beads increased with dissolved oxygen concentration and reached to the higest value 293.96μm at pH=7, meanwhile, the corresponding COD removal rate reached to the maximum which is 64.83%, when the pH range from 6to 9. The light deflection value of the beads decreased with the concentration of Cr3+ and reached to 269.7μm when the Cr3+ concentration at 20mg/L. The light deflection value increased with Cr3+ concentration continiued increased to 50mg/L, but the corresponding COD removal rate decreased form 52.5% to 25.73%, for the reason that the microbial cells are damaged and the intracellular substances are dissolved. The results show that the changes information of metabolic state in activated sludge can be quickly obtained by this method. And the changes of light deflection value of beads can be used to predict the subsequent COD concentration removal effect of printing and dyeing wastewater under specific conditions. Three-dimensional fluorescence spectroscopy were also used to explore the mechanism of microbial metabolism triggering light deflection. And the results showed that the main organic substances involved in microbial metabolism are tyrosine, aromatic proteins and tryptophan.
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