Phosphorus morphology transformation law and sludge characteristics of AOA-SBR process under different C/P
QU Hong1, SHI Xue-ying1, NIE Ze-bing1,2, LI Qing-zhe1,3, LIU Wen-ai1, YU Ge1, MA Yun-guang1, BIAN De-jun1,2
1. Changchun Institute of Technology, Key Laboratory of Jilin Urban Sewage Treatment, Changchun 130012, China; 2. Science and Technology Innovation Center for Municipal Wastewater Treatment and Water Quality Protection, School of Environment, Northeast Normal University, Changchun 130117, China; 3. China Northeast Municipal Engineering Design and Research Institute, Changchun 130021, China
Abstract：Five groups of SBR reactors operating in anaerobic, aerobic, anoxic(AOA) mode were used to investigate the transformation rules of phosphorus morphology and sludge characteristics of AOA-SBR system under different C/P(120, 40, 24, 17, 13). The results showed that the removal effect of COD, TN and NH4+-N all outperformed the primary A standard. As the C/P decreased, the system TP removal gradually increased. When the C/P was less than 24, the removal effect of the TP began to deteriorate and fluctuate violently. When the C/P was 40, the system was the most stable, with an average removal rate of 99.22%. The results showed that with the decrease of C/P, the phosphorus forms in the sludge increased. IP was the main phosphorus form of TP, and IP was similar under different C/P (about 96%). As C/P decreased, the content of sludge TP and IP decreased, while OP content of sludge TP maintained a downward trend. Biological effective phosphorus (the sum of NAIP and OP) had the greatest impact on sewage phosphorus removal effect, with the highest proportion of bioeffective phosphorus in the sludge with C/P 40, and the best sewage TP removal effect. MLSS and SVI increased with C/P, while the risk of sludge expansion the lower C/P systems. High-throughput sequencing showed that with the decrease of C/P, both PAOs and DPB content decreased except for Dechloromonas, and the sewage TP removal effect was poor and fluctuated greatly.
曲红, 石雪颖, 聂泽兵, 李清哲, 刘文爱, 于鸽, 马云广, 边德军. 不同C/P下AOA-SBR工艺磷形态转化规律及污泥特性[J]. 中国环境科学, 2022, 42(1): 92-101.
QU Hong, SHI Xue-ying, NIE Ze-bing, LI Qing-zhe, LIU Wen-ai, YU Ge, MA Yun-guang, BIAN De-jun. Phosphorus morphology transformation law and sludge characteristics of AOA-SBR process under different C/P. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(1): 92-101.
Henze M. Capabilities of biological nitrogen removal processes from wastewater[J]. Water Science and Technology, 1991, 23(4-6): 669-679.
Panswad T, Tongkhammak N, Anotai J. Estimstion of intracellular phosphorus content of phosphorus-accumulating organisms at different P: COD feeding ratios[J]. Journal of Environmental Management, 2007, 84: 141-145.
贾辛慧, 王一冰. 碳磷比对新型后置缺氧工艺脱氮除磷的影响探究[J]. 水处理技术, 2021, 47(4): 115-120. Jia X H, Wang Y B. Effect of carbon and phosphorus ratio on nitrogen and phosphorus removal by new type of post-anoxia process[J]. Technology of Water Treatment, 2021, 47(4): 115-120.
Tsuneda S, Ohno T, Soejima K, et al. Simultaneous nitrogen and phosphorus removal using denitrifying phosphate-accumulating organisms in a sequencing batch reactor[J]. Biochemical Engineering Journal, 2006, 27(3): 191-196.
国家环保总局《水和废水监测分析方法》编委会. 水和废水监测分析方法[M]. 第四版增补版. 北京: 中国环境科学出版社, 2006: 210-227. "Water and wastewater monitoring and analysis method"editorial board of State Environmental Protection Administration of China. Monitoring and analysis methods of water and wastewater [M]. fourth edition. Beijing: China Environmental Science Press, 2006: 210-227.
GB 18918-2002城镇污水处理厂污染物排放标准[S]. GB 18918-2002Pollutant discharge standard for municipal wastewater treatment plant[S].
许德超, 陈洪波, 李小明, 等. 静置/好氧/缺氧序批式反应器(SBR)脱氮除磷效果研究[J]. 环境科学学报, 2014, 34(1): 152-159. Xu D C, Chen H B, Li X M, et al. Biological phosphorus and nitrogen removal in a sequencing batch reactor (SBR) operated in static/ aerobic/ anoxic regime[J]. Acta Scientiae Circumstantiae, 2014, 34(1): 152-159.
Coats E R, Mockos A, Loge F J. Post-anoxic denitrification driven by PHA and glycogen within enhanced biological phosphorus removal[J]. Bioresource Technology, 2011, 102(2): 1019-1027.
Thongchai P, Napaporn T, Jin A. Estimation of intracellular phosphorus content of phosphorus -accumulating organisms at different P: COD feeding ratios[J]. Journal of Environmental Management, 2007, 84(2): 141-145.
张娜. A2O-MBNR工艺中微生物性能及碳磷比对除磷性能的影响研究[D]. 苏州: 苏州科技学院, 2012. Zhang N. Research on the microbial performance and effects of C/P ratio on phosphorus removal in A2O -MBNR process. [D]. Suzhou: Suzhou University of Science and Technology, 2012.
Ruban V, López-Sánchez J F, Pardo P, et al. Selection and evaluation of sequential extraction procedures for the determination of phosphorus forms in lake sediment[J]. Journal of environmental monitoring, 1999, 1(1): 51-56.
Ruban V, López-Sánchez J F, Pardo P, et al. Harmonized protocol and certified reference material for the determination of extractable contents of phosphorus in freshwater sediments–A synthesis of recent works[J]. Fresenius' Journal of Analytical Chemistry, 2001, 370(2/3): 224-228.
Jing L D, Liu X L, Bai S, et al. Effects of sediment dredging on internal phosphorus: A comparative field study focused on iron and phosphorus forms in sediments[J]. Ecological Engineering, 2015, 82: 267-271.
Chen S Y, Chen Y Y, Liu J Z, Zhang J, et al. Vertical variation of phosphorus forms in core sediments from Dongping Lake, China[J]. Procedia Environmental Sciences, 2011, 10: 1797-1801.
Sudiana I M, Mino T, Satoh H, et al. Metabolism of en-hanced biological phosphorus removal and non-enhanced biological phosphorus removal sludge with acetate and glucose as carbon source[J]. Water Science Technology, 1999, 39(6): 29-35.
沈雪莲, 周振, 任伟超, 等. 城镇污水处理厂污泥中磷的形态分布及生物可利用性分析[J]. 环境工程学报, 2016, 10(3): 1200-1204. Shen X L, Zhou Z, Ren W C, et al. Fractionation and bioavailability of phosphorus in sludge from municipal wastewater treatment plants[J]. Chinese Journal of Environmental Engineering, 2016, 10(3): 1200-1204.
王超, 刘清伟, 职音, 等. 中国市政污泥中磷的含量与形态分布[J]. 环境科学, 2019, 40(4): 1922-1930. Wang C, Li Q W, Zhi Y, et al. Contents and forms of phosphorous in the municipal sewage sludge of China[J]. Environmental Science, 2016, 10(3): 1200-1204.
王方舟, 刘雪瑜, 肖书虎, 等. 污水处理厂脱水污泥中磷的形态及其溶出规律[J]. 环境工程学报, 2020, 14(4): 1067-1074. Wang F Z, Liu X Y, Xiao S H, et al. Species and release of phosphorus from dewatered sludge in sewage treatment plants[J]. Chinese Journal of Environmental Engineering, 2020, 14(4): 1067-1074.
杨小梅. 强化生物除磷系统中形态磷的分布及去除途径研究[D]. 苏州: 苏州科技大学, 2016. Yang X M. Research of distribution of phosphorus forms and remove ways in enhanced biological phosphorus removal system [D]. Suzhou: Suzhou University of Science and Technology, 2016.
Wang P, He M C, Lin C Y, et al. Phosphorus distribution in the estuarine sediments of the Daliao River, China[J]. Esuarine, Coastal and Shelf Science, 2009, 84(2): 246-252.
郝王娟, 薛涛, 黄霞. 进水磷碳比对聚磷菌与聚糖菌竞争生长的影响[J]. 中国给水排水, 2007, 23(17): 95-98. Hao W J, Xue T, Huang X. Effect of influent P/C ratio on competition between phosphate and glycogen-accumulating organisms[J]. China Water & Wastewater, 2007, 23(17): 95-98.
周娜, 袁林江, 陈光秀, 等. 污泥含磷量与脱氮除磷系统污泥膨胀的关系研究[J]. 环境科学, 2009, 30(10): 2981-2987. Zhou N, Yuan L J, Chen G X, et al. Relationship between phosphorus content in activated sludge and sludge bulking in biological nutrient removal SBR systems[J]. Environmental Science, 2009, 30(10): 2981-2987.
方振东, 龙向宇, 唐然, 等. 胞外聚合物结合磷效能的研究[J]. 环境科学学报, 2011, 31(11): 2374-2379. Fang Z D, Long X Y, Tang R, et al. The phosphorus-incorporating property of extracellular polymer substances[J]. Acta Scientiae Circumstantiae, 2011, 31(11): 2374-2379.
郭超, 刘怀英, 王琪, 等. 除磷颗粒污泥中磷的形态及其含量分析[J]. 安全与环境工程, 2015, 22(2): 44-48. Guo C, Liu H Y, Wang Q, et al. Existing morphology of phosphorus in dephosphorization granular sludge and its content analysis[J]. Safety and Environmental Engineering, 2015, 22(2): 44-48.
Sommers L F, Harris R F, Willianms J D H, et al. Fractionation of organic phosphorus in lake sediments[J]. Soil Science Society of America Journal, 1972, 36(1): 51-54.
Kaiserli A, Voutsa D, Samara C. Phosphorus fractionation in lake sediments–Lakes Volvi and Koronia, N. Greece[J]. Chemosphere, 2002, 46(8): 1147-1155.
Andreasen K, Sigvarden L. Experiments with sludge bulking in the UK[J]. Water Environment, 2004, 18(3): 177-182.
邵基伦. Burkholderia菌异养硝化—好氧反硝化特性及其强化废水处理的研究[D]. 广州: 暨南大学, 2015. Shao J L. Heterotrophic nitrification and aerobic denitrification characteristics of burkholderia strain and its enhancing wastewater treatment [D]. Guangzhou: Jinan University, 2015.
杨垒, 陈宁, 任勇翔, 等. 异养硝化细菌Acinetobacter junii NP1的同步脱氮除磷特性及动力学分析[J]. 环境科学, 2019, 40(8): 3713-3721. Yang L, Chen N, Ren Y X, et al. Simultaneous nitrogen and phosphorus removal and kinetics by the heterotrophic nitrifying bacterium acinetobacter junii NP1[J]. Environmental Science, 2019, 40(8): 3713-3721.
郝晓地, 陈峤, 刘然彬. Tetrasphaera聚磷菌研究进展及其除磷能力辨析[J]. 环境科学学报, 2020, 40(3): 741-753. Hao X D, Chen Q, Liu R B. Research advances of Tetrasphaera as polyphosphate accumulating organisms and analysis on their P-removal potential[J]. Acta Scientiae Circumstantiae, 2020, 40(3): 741-753.
何冬兰, 詹锐, 万文结, 等. 一株厌氧型聚磷菌的分离鉴定与聚磷特性[J]. 中南民族大学学报(自然科学版), 2016, 35(2): 23-25, 50. He D, Zhan R, Wan W J, et al. Isolation and characteristics of an anaerobic phosphate-accumulating bacterium strain[J]. Journal of South-Central University for Nationalities (Natural Science Edition), 2016, 35(2): 23-25, 50.