PFOA与PFOS对厌氧氨氧化污泥特性和微生物群落的影响

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摘要通过序批实验研究了不同浓度(0.5, 1mg/L)与不同类型的全氟化合物(PFCs)对厌氧氨氧化(anammox)污泥脱氮性能及微生物群落的影响.结果表明, 0.5与1mg/L全氟辛酸(PFOA)和全氟辛烷磺酸盐(PFOS)对anammox污泥的脱氮性能无明显抑制作用; 1mg/L PFOA(OA1)与PFOS(OS1)添加至anammox污泥中后1d, 其去除率分别达到47.68%和92.7%.污泥的X射线光电子能谱(XPS)分析表明OA1、OS1实验组存在C-F、MgF₂、CaF₂等官能团.PFOA和PFOS胁迫下anammox污泥中血红素c浓度出现降低, OA1与OS1实验组分别降低了21.05%、7.5%.对不同实验组anammox污泥进行高通量测序分析表明, 1mg/L PFOA和PFOS的添加会降低厌氧氨氧化菌但促进反硝化细菌的相对丰度, OA1、OS1实验组中Candidatus Brocadia属的相对丰度分别降低1.08%、0.28%, 而不动杆菌属Acinetobacter相对丰度增加1.73%与0.06%.整体而言, PFOA对anammox污泥的负面影响更为明显.

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	唐琳钦
	宿程远
	黄娴
	李汝婷
	王安柳
	樊翠萍
	先云川

关键词 ：全氟辛酸, 全氟辛烷磺酸盐, 厌氧氨氧化, 污泥特性, 微生物群落

Abstract：The sequential batch experiments explored the influence mechanism under different concentrations (0.5mg/L and 1mg/L) and types of perfluorinated compounds (PFCs) on the denitrification performance and microbial community of anaerobic ammonia oxidation (anammox) sludge. Results indicated that perfluorooctanoic acid (PFOA) (at 0.5mg/L, 1mg/L) and perfluorooctane sulfonate (PFOS) had no obvious inhibitory effect on the denitrification performance of the anammox sludge. The addition of 1mg/L PFOA (OA1) and PFOS (OS1) to the anammox sludge for one day resulted in removal rates of respectively 47.68% and 92.7%. X-ray photoelectron spectroscopy (XPS) analysis of the sludge showed the presence of C-F, MgF₂, CaF₂ functional groups in the OA1 and OS1 groups. The addition of PFOA and PFOS reduced the concentration of heme c in the anammox sludge by respectively 21.05% and 7.5%. The high-throughput sequencing analysis of the anammox sludge in different experimental groups showed that the addition of 1mg/L PFOA and PFOS could reduce the relative abundance of anammox bacteria and promote the relative abundance of denitrifying bacteria. The relative abundance of Candidatus Brocadia in the OA1 and OS1 groups decreased by respectively 1.08% and 0.28%, while the relative abundance of Acinetobacter increased by 1.73% and 0.06%. In general, the negative effects of PFOA on the anammox sludge were more significant than PFOS.

Key words： perfluorooctanoic acid perfluorooctane sulfonate anammox sludge characteristics microbial communities

收稿日期: 2021-06-01

PACS:

X703.1

基金资助:国家自然科学基金资助项目(52060003);广西师范大学科研育人项目(2020YR009)

通讯作者: 宿程远, 教授, suchengyuan2008@126.com E-mail: suchengyuan2008@126.com

作者简介: 唐琳钦(1997-), 男, 广西桂林人, 广西师范大学硕士研究生, 研究方向为水处理技术

引用本文:

唐琳钦, 宿程远, 黄娴, 李汝婷, 王安柳, 樊翠萍, 先云川. PFOA与PFOS对厌氧氨氧化污泥特性和微生物群落的影响[J]. 中国环境科学, 2022, 42(1): 194-202. TANG Lin-qin, SU Cheng-yuan, HUANG Xian, LI Ru-ting, WANG An-liu, FAN Cui-ping, XIAN Yun-chuan. Evaluation of sludge characteristics and microbial community of anammox sludge during exposure to perfluorooctane acid and perfluorooctane sulfonate. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(1): 194-202.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2022/V42/I1/194

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