Effect of EDTA-2Na/Fe(Ⅱ) on nitrate reduction in autotrophic biological system
ZHANG Wen-bo1, LI Xiao-ling1,2, LIU Xin-yi1, ZHANG Jia-ying1, ZHOU Jun-cai1, MENG Wen1, ZHANG Peng-cheng1
1. School of Civil Engineering, Chang'an University, Xi'an 710061, China; 2. Key Laboratory of Water Supply & Sewage Engineering, Ministry of Housing and Urban-Rural Development, School of Civil Engineering, Chang'an University, Xi'an 710054, China
Abstract:The initiation of iron mediated denitrification was studied in an anaerobic sequenced batch biofilm reactor (ASBBR) with a fixed concentration of nitrate and ferrous sulfate, supplemented with EDTA-2Na according to different gradient conditions for a long period of cultivation domestication. Meanwhile, the effects of different EDTA-2Na/Fe(Ⅱ) on iron autotrophic denitrification process and the dissimilatory nitrate reduction to ammonium (DNRA) were also explored in this study. The results showed that after 65days of cultivation and domestication, the reactor operated successfully and stably, and the removal rate of NO3--N was up to 99.70%. When EDTA-2Na/Fe(Ⅱ) < 1.50, only iron autotrophic denitrification was performed with the highest removal rate of NO3--N of 71.70%; When EDTA-2Na/Fe(Ⅱ) ≥1.50, iron autotrophic denitrification and DNRA were carried out simultaneously in the reactor, and the highest removal rate of NO3--N was 99.70%. It was worth noting that when EDTA-2Na/Fe(Ⅱ)=1.50, the iron autotrophic denitrification rate reached the maximum value of 1.63mg/(L·h), and the ammonia production of DNRA also reached the maximum value of 9.75mg/L. Visual MINTEQ simulation results showed that the molar ratio of EDTA-2Na to Fe (Ⅱ) affected the existing forms of EDTA-2Na and Fe (Ⅱ) in the influent. The larger the molar ratio, the higher the concentration of FeEDTA2- and the stronger the bioavailability of Fe (Ⅱ). Thr ough microbial population analysis, it was found that the dominant bacteria were Brucella, Castellaniella, Ochrobactrum, Pseudomonas as well as Citrobacter. Among them the first four bacteria were related to denitrification process, while Citrobacter was related to DNRA process and was only appeared under the conditions of EDTA-2Na/Fe(Ⅱ)=1.50 and 1.75 in this research. Therefore, the above results could throw light on further exploring the relationship between DNRA and denitrification.
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