包埋硫铁生物填料的制备及自养反硝化性能

摘要
图/表
参考文献
相关文章 (8)

全文: PDF (838 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要采用包埋固定化技术制备了包埋硫铁生物填料(ESI Filler),基于升流式自养反硝化反应器开展动态实验研究,通过改变水力停留时间(HRT)、pH值、溶解氧(DO)等运行条件,探究ESI Filler反应器的脱氮效果及微生物群落结构组成。结果表明,当进水硝酸盐氮(NO₃^--N)浓度为30mg/L,HRT为10h时,NO₃^--N去除率不断上升至99.80%。当HRT缩短为2.5h时,NO₃^--N去除率降至61.35%。ESI Filler反应器对pH值和DO的改变具有较高的稳定性,NO₃^--N平去除率可维持在82.5%以上。但对低温的耐受性较差,当温度从35℃降低至15℃时,NO₃^--N平均去除率由90.12%降低至68.80%。运行164d后,球体未出现破裂散落的现象,表现出较长的使用寿命。通过扫描电镜发现,填料表面疏松多孔,附着大量杆状细菌,已成为微生物的良好载体。高通量测序结果表明,包埋颗粒中优势菌属为典型的自养反硝化功能菌Thiobacillus,丰度为80.79%。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	刘艳芳
	刘晓帅
	尹思婕
	高玮
	张妙雨
	韩严和
	李再兴

关键词 ：硫自养反硝化, 铁自养反硝化, 包埋填料, 脱氮稳定性, 微生物特性

Abstract：The embedded sulfur/iron biological filler (ESI Filler) was prepared by embedding and immobilization technology, and dynamic experimental research was carried out based on the upflow autotrophic denitrification reactor. By changing hydraulic retention time (HRT), pH, dissolved oxygen (DO) and other operating conditions, the denitrification performance and microbial community structure composition of ESI Filler reactor were explored. When the influent nitrate nitrogen (NO₃^--N) concentration was 30mg/L and the HRT was 10h, the NO₃^--N removal rate increased continuously to 99.80%. When the HRT was shortened to 2.5h, the removal rate of NO₃^--N decreased to about 61.35%. ESI Filler reactor had high stability to the changes of pH and DO, and the average removal rate of NO₃^--N could be maintained above 82.5%. However, the tolerance to low temperature was poor. When the temperature was reduced from 35℃ to 15℃, the average removal rate of NO₃^--N decreased from 90.12% to 68.80%. After running for 164days, the spheres did not break and scatter, showing a long service life. It was found by scanning electron microscopy that the surface of the filler was loose and porous, and a large number of rod-shaped bacteria were attached, which had become a good carrier of microorganisms. The results of high-throughput sequencing showed that the dominant bacterial genus in the embedded granules was the typical autotrophic denitrifying bacteria Thiobacillus, with an abundance of 80.79%.

Key words： sulfur autotrophic denitrification iron autotrophic denitrification embedding filler denitrification stability microbial properties

收稿日期: 2022-04-06

PACS:

X703.1

基金资助:河北省重点研发计划项目(19273601D)

通讯作者: 李再兴,教授,li_zaixing@163.com E-mail: li_zaixing@163.com

作者简介: 刘艳芳(1984-),女,河北石家庄人,副教授,博士,主要从事水污染控制技术研究。发表论文20余篇。

引用本文:

刘艳芳, 刘晓帅, 尹思婕, 高玮, 张妙雨, 韩严和, 李再兴. 包埋硫铁生物填料的制备及自养反硝化性能[J]. 中国环境科学, 2022, 42(11): 5136-5143. LIU Yan-fang, LIU Xiao-shuai, YIN Si-jie, GAO Wei, ZHANG Miao-yu, HAN Yan-he, LI Zai-xing. Preparation and autotrophic denitrification properties of embedded sulfur/iron biological filler. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(11): 5136-5143.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2022/V42/I11/5136

[1]	付炳炳,潘建新,马景德,等.采用含硫铁化学污泥作为反硝化电子供体进行焦化废水中总氮深度去除[J]. 环境科学, 2018,39(7):3262-3270. Fu B B, Pan J X, Ma J D, et al. Evaluation of advanced nitrogen removal from coking wastewater using sulfide iron-containing sludge as a denitrification electron donor[J]. Environmental Science, 2018, 39(7):3262-3270.
[2]	周彦卿,郝瑞霞,刘思远,等.新型硫铁复合填料强化再生水深度脱氮除磷[J]. 环境科学, 2017,38(10):4309-4315. Zhou Y Q, Hao R X, Liu S Y, et al. improving nitrogen and phosphorus removal from reclaimed water using a novel sulfur/iron composite filler[J]. Environmental Science, 2017,38(10):4309-4315.
[3]	Zhang L L, Song Y D, Zuo Y, et al. Integrated sulfur-and iron-based autotrophic denitrification process and microbial profiling in an anoxic fluidized-bed membrane bioreactor[J]. Chemosphere, 2019,221:375-382.
[4]	Vo T, Kang S, An S, et al. Exploring critical factors influencing on autotrophic denitrification by elemental sulfur-based carriers in upflow packed-bed bioreactors[J]. Journal of Water Process Engineering, 2021,101866.
[5]	Xu Y X, Chen N, Feng C P, et al. Sulfur-based autotrophic denitrification with eggshell for nitrate-contaminated synthetic groundwater treatment[J]. Environmental Technology, 2016,37(21-24):3094-3103.
[6]	Shin K H, Cha D K. Microbial reduction of nitrate in the presence of nanoscale zero-valent iron[J]. Chemosphere, 2008,72(2):257-262.
[7]	Peng L, Liu Y, Gao S H, et al. Evaluation on the nanoscale zero valent iron based microbial denitrification for nitrate removal from groundwater[J]. Scientific Reports, 2015,5:1-11.
[8]	Wang J J, Huang B C, Li J, et al. Advances and challenges of sulfur-driven autotrophic denitrification (SDAD) for nitrogen removal[J]. Chinese Chemical Letters, 2020,31(10):2567-2574.
[9]	宋佳强,李军,陈光辉,等.pH值对包埋固定化技术反硝化脱氮的影响[J]. 中国给水排水, 2019,35(3):78-82. Song J Q, Li J, Chen G H, et al. Influence of pH on denitrification with embedded immobilized technology[J]. China Water & Wastewater, 2019,35(3):78-82.
[10]	Lin Y H, Hwang S J, Wu J Y, et al. Simultaneous removal of carbon and nitrogen from swine wastewater using an immobilized-cell reactor[J]. Journal of Environmental Engineering, 2006,132(3):423-429.
[11]	Sun S Y, Fan S L, Shen K X, et al. Laboratory assessment of bioleaching of shallow eutrophic sediment by immobilized pH值otosynthetic bacteria[J]. Environmental Science and Pollution Research International, 2017,24(28):22143-22151.
[12]	宋佳强,李军,陈光辉,等.利用包埋固定化技术进行反硝化脱氮的研究[J]. 中国给水排水, 2018,34(23):105-110. Song J Q, Li J, Chen G H, et al. Denitrification Using Embedded Immobilized Denitrifying Bacteria[J]. China Water & Wastewater, 2018,34(23):105-110.
[13]	周亚坤,杨宏,王少伦,等.包埋反硝化填料强化二级出水深度脱氮性能研究及中试应用[J]. 环境科学, 2020,41(2):849-855. Zhou Y K, Yang H, Wang S L, et al. Research on denitrification performance of enhanced secondary effluent by embedded denitrification filler and pilot application[J]. Environmental Science, 2020,41(2):849-855.
[14]	Beller, Anaerobic H R. Nitrate-dependent oxidation of U(IV) oxide minerals by the chemolithoautotrophic bacterium thiobacillus denitrificans[J]. Applied & Environmental Microbiology, 2005,71(4):2170-4.
[15]	Cui Y X, Guo G G, Biswal B K, et al. Investigation on sulfide-oxidizing autotrophic denitrification in moving-bed biofilm reactors:an innovative approach and mechanism for the process start-up[J]. International Biodeterioration & Biodegradation, 2019,140:90-98.
[16]	Wang Z, Fei X, He S B, et al. Effects of hydraulic retention time and S2O32-/NO3-ratio on thiosulfate-driven autotrophic denitrification for nitrate removal from micro-polluted surface water.[J]. Environmental technology, 2017,38(22):2835-2843.
[17]	Deng S H, Li D S, Yang X, et al. Novel characteristics on micro-electrolysis mediated Fe(0)-oxidizing autotrophic denitrification with aeration:efficiency, iron-compounds transformation, N2O and NO2-accumulation, and microbial characteristics[J]. Chemical Engineering Journal, 2020,387:123409-123409.
[18]	Zhang M, Zhangzhu G C, Wen S X, et al. Chemolithotrophic denitrification by nitrate-dependent anaerobic iron oxidizing (NAIO) process:insights into the evaluation of seeding sludge[J]. Chemical Engineering Journal, 2018,345:345-352.
[19]	Baalsrud K S. Studies on thiobacillus denitrificans[J]. Archiv Fur Microbiologie, 1954,20(1):34-62.
[20]	Moon H S, Shin D Y, Nam K, et al. A long-term performance test on an autotrophic denitrification column for application as a permeable reactive barrier[J]. Chemosphere, 2008,73(5):723-728.
[21]	Sahinkaya E, Dursun N, Kilic A, et al. Simultaneous heterotrophic and sulfur-oxidizing autotrophic denitrification process for drinking water treatment:Control of sulfate production[J]. Water Research, 2011, 45(20):6661-6667.
[22]	任争鸣,刘雪洁,苏晓磊,等.硫自养反硝化深度脱氮中试研究[J]. 中国给水排水, 2016,32(19):31-35. Ren Z M, Liu X J, Su X L, et al. Sulfur-based autotrophic denitrification process for advanced nitrogen removal[J]. China Water & Wastewater, 2016,32(19):31-35.
[23]	于妍,刘宁,廖祖刚,等.铁型反硝化脱氮技术研究进展[J]. 中国环境科学, 2022,42(1):83-91. Yu Y, Liu N, Liao Z G, et al. Research progress of iron-type denitrification removal technology[J]. China Environmental Science, 2022,42(1):83-91.
[24]	Sahinkaya E, Dursun N, Kilic A, et al. Simultaneous heterotrophic and sulfur-oxidizing autotrophic denitrification process for drinking water treatment:Control of sulfate production[J]. Water Research, 2011, 45(20):6661-6667.
[25]	Liu L, Koenig A. Use of limestone for pH control in autotrophic denitrification:batch experiments[J]. Process Biochemistry, 2002, 37(8):885-893.
[26]	许保玖,龙腾锐.当代给水与废水处理原理.第2版[M]. 北京:高等教育出版社, 2000:42-96. Xu B J, Long T R. Contemporary principles of water and wastewater treatment. 3rd Edition[M]. Beijing:Higher Education Press, 2000:42-96.
[27]	周群英,王士芬.环境工程微生物学.第3版[M]. 北京:高等教育出版社, 2008:109-224. Zhou Q Y, Wang S F. Environmental engineering microbiology. 3rd Edition[M]. Beijing:Higher Education Press, 2008:109-224.
[28]	Qambrani N A, Oh S E. Effect of dissolved oxygen tension and agitation rates on sulfur-utilizing autotrophic denitrification:batch tests[J]. Applied Biochemistry and Biotechnology, 2013,169(1):181-91.
[29]	张雯.以农业废弃物为基料的地下水反硝化缓释碳源材料研究及应用[D]. 南京:南京大学, 2017. Zhang W. The research and application of sustainable long-release carbon material with agricultural waste[D]. Nanjing:Nanjing University, 2017.
[30]	Fajardo C, Mora M, Fernández I, et al. Cross effect of temperature, pH and free ammonia on autotrophic denitrification process with sulphide as electron donor[J]. Chemosphere, 2014,97:10-15.
[31]	Zhou W L, Liu X, Dong X J, et al. Sulfur-based autotrophic denitrification from the micro-polluted Water[J]. Journal of Environmental Sciences, 2016,44(6):180-188.
[32]	马潇然,郑照明,卞伟,等.硫自养反硝化系统运行效能和微生物群落结构研究[J]. 中国环境科学, 2020,40(10):4335-4341. Ma X R, Zheng Z M, Bian W, et al. Study on operation efficiency and microbial community structure of sulfur-based autotrophic denitrification system[J]. 2020,40(10):4335-4341.
[33]	Xu X C, Zhang R, Jiang H B, et al. Sulphur-based autotrophic denitrification of wastewater obtained following graphite production:long-term performance, microbial communities involved, and functional gene analysis[J]. Bioresource Technology, 2020,306:123117.
[34]	吕小梅,吴毅聪,陈桂连,等.硫自养反硝化颗粒表面与间隙微生物群落特征和基因分布[J]. 中国环境科学, 2022,42(6):2764-2770. Lv X M, Wu Y C, Chen G L, et al. Community structure and gene distribution of the surface and interstitial biofilm in the particle sulfur autotrophic denitrification.
[35]	Yu L P, Yuan Y, Chen S S, et al. Direct uptake of electrode electrons for autotrophic denitrification by thiobacillus denitrificans[J]. Electrochemistry Communications, 2015,60:126-130.
[36]	Hu Q T, Wang S S, Lai Q L, et al. Sulfurimonas Indica sp. nov., a hydrogen-and sulfur-oxidizing chemolithoautotroph isolated from a hydrothermal sulfide chimney in the northwest indian ocean[J]. International Journal of Systematic and Evolutionary Microbiology, 2020,71(1):004575.