外源硫诱导下的<i>Desulfovibrio desulfuricans sub</i> sp.EPS特性及对Zn(Ⅱ)的吸附

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摘要研究了3种外源硫(Na₂SO₄、Na₂SO₃和Na₂S₂O₃·5H₂O)对Desulfovibrio desulfuricans sub sp.(D.desulfuricans sp.)的胞外聚合物(EPS)的胁迫/诱导作用。结果表明,在还原性硫源0.50g/L Na₂SO₃的条件下,EPS产量最高,为2104.39mg/g VSS,蛋白质含量为1888.52mg/g VSS,较胁迫/诱导前均提高了300%以上;其对Zn(Ⅱ)的吸附性能最好,为954.4mg/g EPS,提高了98.17%。三维荧光(3D-EEM)结果表明,胁迫/诱导后EPS中类酪氨酸均大量增加;傅里叶红外光谱(FTIR)结果表明,胁迫后-OH、C=O、C-O-C等官能团均大量增加,在Zn(Ⅱ)的吸附中发挥了重要作用;X光电子能谱(XPS)结果表明,在还原性硫源(Na₂SO₃和Na₂S₂O₃·5H₂O)胁迫/诱导后,EPS中C-O/C-N、C=N和某种含氧基团(X)大量增加,可能是吸附Zn(Ⅱ)的主要基团。

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	甘雨
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	连泽阳
	马双念
	黄祥武
	羊仁高
	温炎标

关键词 ：外源硫, 胁迫/诱导, EPS, Zn(Ⅱ)

Abstract：In this paper, the effect of exogenous sulfur (Na₂SO₄, Na₂SO₃, Na₂S₂O₃·5H₂O) stress/induction on extracellular polymeric substances(EPS) of Desulfovibrio desulfuricans sub sp. (D. desulfuricans sp.) was studied. The results showed that addition of 0.50g/L Na₂SO₃ led to the highest EPS yield of 2104.39mg/g VSS (1888.52mg/g VSS of protein content), which was 300% higher than that without Na₂SO₃ addition, and as a result, 98.17% increase in Zn(II) adsorption capacity (954.4mg/g EPS) achieved. Three-dimensional fluorescence (3D-EEM) spectral results showed that tyrosine-like substances in EPS were greatly increased after sulfur stress/induction; Fourier transform infrared spectroscopy (FTIR) results showed that significant increases in functional groups such as —OH, C=O, C—O—C EPS were mainly responsible for the enhance adsorption of Zn(II); The results of X-ray photoelectron spectroscopy (XPS) results showed that the content of C—O/C—N, C=N and oxygen-containing functional group (X) in EPS increased after sulfur stress/induction (Na₂SO₃ and Na₂S₂O₃·5H₂O).

Key words： exogenous sulfur stress/induction EPS Zn(Ⅱ)

收稿日期: 2022-04-18

PACS:

X172

基金资助:广东省自然科学基金资助项目(2021A1515010558)

通讯作者: 宋卫锋,教授,weifengsong@gdut.edu.cn E-mail: weifengsong@gdut.edu.cn

作者简介: 甘雨(1997-),男,广东惠州人,广东工业大学硕士研究生,主要从事EPS吸附重金属及介导合成金属硫化物。发表论文2篇。

引用本文:

甘雨, 宋卫锋, 杨佐毅, 连泽阳, 马双念, 黄祥武, 羊仁高, 温炎标. 外源硫诱导下的Desulfovibrio desulfuricans sub sp.EPS特性及对Zn(Ⅱ)的吸附[J]. 中国环境科学, 2022, 42(11): 5144-5152. GAN Yu, SONG Wei-feng, YANG Zuo-yi, LIAN Ze-yang, MA Shuang-nian, HUANG Xiang-wu, YANG Ren-gao, WEN Yan-biao. The EPS characteristics of Desulfovibrio desulfuricans sub sp. and its adsorption performance for Zn(Ⅱ) under exogenous sulfur induction. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(11): 5144-5152.

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