Orthogonal experiment to optimize vertical subsurface flow constructed wetland for short-cut nitrification
YAN Yuan1,2,3, MA Jiao1,2,3, DANG Hong-zhong1,2,3, WU Xin-bo1,2,3, ZENG Tian-xu1,2,3, LI Wei-wei1,2,3,4, CHEN Yong-zhi1,2,3
1. Key Laboratory of Yellow River Water Environment in Gansu Province, Lanzhou Jiaotong University, Lanzhou 730070, China; 2. School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China; 3. Technical Center of Sewage Treatment Industry in Gansu Province, Lanzhou 730070, China; 4. Gansu Research Institute of Light Industry Co., Ltd, Lanzhou 730070, China
Abstract：Vertical subsurface flow constructed wetland (VSFCW) was used to treat domestic wastewater and short-cut nitrification was achieved. When the temperature, pH value, dissolved oxygen (DO), and hydraulic retention time (HRT) were maintained at (30±2)℃, (8.4±0.2), (1.5±0.2) mg/L, and 12h, respectively. The removal efficiency of COD and ammonia removal efficiency (ARE) were 73.14%, 98.15%, respectively. However, nitrite accumulation rate (NAR) was 65.18%. The NAR can be improved further by adding hydroxylamine (NH2OH) to VSFCW. A 4-factor, 5-level orthogonal experiment was designed to determine the optimal control conditions of the NH2OH concentration, DO, temperature, and pH value. In this study, the analysis of variance (ANOVA) results revealed that the effects of NH2OH concentration and temperature on NAR were extremely significant (P≤0.01). DO and pH value had no significant effect on NAR (P>0.05). Even if the temperature was dropped from 30℃ to 23℃, the NAR could reach 89.15% by adding 1.0mg/L NH2OH. And the removal efficiency of COD and ARE were 75.67% and 88.76%, respectively. The abundance of Nitrosomonas increased from 1.62% to 4.06%, and the abundance of Nitrospira declined from 1.65% to 0.95%.
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